利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

170 条记录 (耗时 0.029 秒)

    Assay of blood or other biologic samples for target analytes
    3.
    发明授权
    Assay of blood or other biologic samples for target analytes 失效
    测定目标分析物的血液或其他生物样品

    公开(公告)号：US5635362A

    公开(公告)日：1997-06-03

    申请号：US247336

    申请日：1994-05-23

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Alvydas J. Ozinskas

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Alvydas J. Ozinskas

    IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/558

    CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

    摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

    摘要翻译： 可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。 每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。 将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。 离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。 通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。 因此，未结合的标签结合材料不会影响分析。

    Determination of white blood cell differential and reticulocyte counts
    5.
    发明授权
    Determination of white blood cell differential and reticulocyte counts 有权
    测定白细胞差异和网织红细胞计数

    公开(公告)号：US06350613B1

    公开(公告)日：2002-02-26

    申请号：US09252153

    申请日：1999-02-18

    申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  Rodolfo R. Rodriguez

    发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  Rodolfo R. Rodriguez

    IPC分类号： G01N3100

    CPC分类号： G01N15/1475 ,  G01N33/5094 ,  G01N2015/0076 ,  G01N2015/0092 ,  G01N2015/1472 ,  G01N2015/1497 ,  Y10T436/10 ,  Y10T436/101666

    摘要： Target nucleated cells, and target cells containing remnant ribosomal material, which are present in a quiescent anticoagulated whole blood sample are optically detected, enumerated, and analyzed in a sample chamber that has a varying through plane thickness due to convergent opposing sample chamber walls. At least one of the convergent walls of the chamber is transparent so that the blood sample can be observed. The chamber's varying thickness produces a first lesser thickness region in the chamber wherein individual red cells and quiescent monolayers of red cells in the sample will reside after the sample is introduced into and fills the chamber. Larger formed constituents such as white blood cells and nucleated red blood cells present in the sample will reside in greater thickness regions of the chamber, and non-nucleated red cells which reside in such greater thickness regions will agglomerate to form rouleaux. By admixing fluorescent dyes with the blood sample, target cells in the sample can be enumerated and differentiated by means of a scanning instrument which is able to measure different wave length color signals emitted from the target cells in the sample, and differentiate the target cells one from another by reason of the nature of the emitted color signals.

    摘要翻译： 目标有核细胞和含有剩余核糖体材料的靶细胞，其存在于静止抗凝全血样品中，由于收敛的相对的样品室壁在具有不同的平面厚度的样品室中进行光学检测，计数和分析。 室的收敛壁中的至少一个是透明的，使得可以观察血液样本。 室的不同厚度在腔室中产生第一较小的厚度区域，其中在将样品引入并填充室之后，样品中红细胞的各个红细胞和静止单层将驻留。 存在于样品中的较大的成形组分如白细胞和有核红细胞将驻留在室的较大厚度区域中，并且驻留在这样较厚的区域中的未成核的红细胞将聚集形成rouleaux。 通过将荧光染料与血液样品混合，样品中的靶细胞可以通过能够测量样品中靶细胞发射的不同波长颜色信号的扫描仪器进行枚举和分化，并将靶细胞分化为一 由于发射的颜色信号的性质，从另一个。

    Assay of blood or other biologic samples for target analytes
    6.
    发明授权
    Assay of blood or other biologic samples for target analytes 失效
    测定目标分析物的血液或其他生物样品

    公开(公告)号：US5776710A

    公开(公告)日：1998-07-07

    申请号：US771507

    申请日：1996-12-23

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

    IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/558

    CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

    摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

    摘要翻译： 可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。 每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。 将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。 离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。 通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。 因此，未结合的标签结合材料不会影响分析。

    Material layer volume determination with correction band
    8.
    发明授权
    Material layer volume determination with correction band 失效
    材料层体积确定与校正带

    公开(公告)号：US4823624A

    公开(公告)日：1989-04-25

    申请号：US248632

    申请日：1988-09-26

    申请人： Rodolfo R. Rodriguez ,  Matthew W. Lesniesky ,  Charles F. Galanaugh ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Theodore Juraschek

    发明人： Rodolfo R. Rodriguez ,  Matthew W. Lesniesky ,  Charles F. Galanaugh ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Theodore Juraschek

    IPC分类号： B01L3/14 ,  G01N15/05 ,  A61B5/14

    CPC分类号： B01L3/50215 ,  G01N15/05

    摘要： A centrifuge tube is used to hold a mixture of several constituents, and also contains a generally cylindrical float. The float settles, after centrifugation, into the zone occupied by the constituent whose volume is to be measured. The constituent layer will settle, after centrifugation, into the annular space between the tube bore and the outside of the float, and will be expanded axially due to the restricted volume of the annular space. The degree of expansion is dependent upon the respective sizes of the float O.D. and the tube bore ID, both of which must be closely controlled for accurate results. A known volume of a control material is placed in the tube to settle into the annular space during centrifugation in an area thereof outside of the constituent layer zone. The length of the band of the control material is measured after centrifugation and is compared to a known length which will result if the annular space is of the proper target volume. A correction factor is thus obtained and applied to all of the other constituent layers which were measured. The band thus forms a varying reference which reflects the actual volume of the annulus.

    摘要翻译： 离心管用于容纳几种成分的混合物，并且还包含一般为圆柱形的浮子。 离心后，浮子沉降到被测量体积所占据的区域内。 构成层在离心后将沉淀到管孔和浮体外部之间的环形空间中，并且由于环形空间的体积受限制而将轴向膨胀。 膨胀程度取决于浮子O.D的各自尺寸。 和管孔ID，两者都必须严格控制以获得准确的结果。 将已知体积的对照材料放置在管中，以便在构成层区域外的区域中离心期间沉降到环形空间中。 在离心后测量对照材料带的长度，并将其与已知的长度进行比较，如果环形空间具有适当的目标体积，则该长度将产生。 因此获得校正因子并将其应用于所测量的所有其它构成层。 因此，带形成变化的参考，其反映环的实际体积。