公开(公告)号：US07682571B2

公开(公告)日：2010-03-23

申请号：US10899143

申请日：2004-07-27

申请人： Sung Jin Kim ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

发明人： Sung Jin Kim ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

IPC分类号： B01L3/00

CPC分类号： B01L3/502707 ,  B01J2219/00286 ,  B01J2219/00389 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00869 ,  B01J2219/00873 ,  B01L7/00 ,  B01L2300/1827 ,  C40B60/14 ,  H05B3/26 ,  H05B2203/003 ,  H05B2203/013 ,  H05B2203/017

摘要： Provided is a micro-fluidic heating system, which comprises a micro-fluidic control element for providing a chamber, a flow path and a valve, and a main body for heating the inside of the chamber in contact with the micro-fluidic control element, wherein the micro-fluidic control element consists of an upper substrate for providing the chamber, the flow path and the valve, and a lower substrate as a thin film bonded to the upper substrate, and the main body consists of a membrane in which heating means and suction holes are formed, and support member for supporting the membrane, and the heating means is partially in contact with the lower substrate of the chamber to heat the inside of the chamber, so that thermal transfer efficiency becomes maximized and temperature of each chamber may be independently controlled in the case of configuration having chambers arranged in array.

摘要翻译： 提供了一种微流体加热系统，其包括用于提供腔室，流路和阀门的微流控制元件和用于加热与微流控制元件接触的腔室内部的主体， 其特征在于，所述微流体控制元件由用于提供所述室，所述流路和所述阀的上基板以及与所述上基板接合的薄膜的下基板构成，所述主体由膜构成，加热单元 并且形成吸入孔，并且用于支撑膜的支撑构件，并且加热装置部分地与室的下基板接触以加热室的内部，使得热传递效率变得最大，并且每个室的温度可以 在配置有排列成阵列的腔室的情况下，可独立控制。

公开(公告)号：US07652370B2

公开(公告)日：2010-01-26

申请号：US10899177

申请日：2004-07-27

申请人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

发明人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

IPC分类号： H01L29/06

CPC分类号： B01J19/0093 ,  B01J2219/00495 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00783 ,  B01J2219/00804 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00858 ,  B01J2219/00873 ,  B01J2219/00961 ,  B01L3/502707 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/1827 ,  B33Y80/00 ,  C40B60/14 ,  G01N27/128

摘要： Provided are a plastic microfabricated structure, and a microfabricated thermal device, a microfabricated reactor, a microfabricated reactor array and a micro array using the same, which may be applied to a bio chip, and the present invention may fabricate the plastic microfabricated structure for providing a heating region by means of insulating plastic, which has a thin thickness, flatness enough to allow a photolithography process to be performed, thermal isolation in its some or total area, and a small thermal mass, and on top of the heating region of the plastic microfabricated structure, a heater, a temperature sensor for sensing a temperature, an electrode, and an electrode pad are formed to thereby fabricate the microfabricated heating device, whereby element may be readily fabricated at a low cost, and the heating region is formed of a plastic thin layer, so that uniform temperature control is possible even with a low power, and various samples may be thermally treated at a fast speed to obtain their reaction and analysis.

摘要翻译： 本发明提供一种塑料微制造结构，微制造热装置，微制造反应器，微制造反应器阵列和使用其的微阵列，其可以应用于生物芯片，并且本发明可以制造塑料微制造结构以提供 通过绝缘塑料的加热区域，其具有薄的厚度，足够的平坦度，以允许执行光刻工艺，其一些或总面积的热隔离和小的热质量，以及在 形成塑料微加工结构，加热器，用于感测温度的温度传感器，电极和电极焊盘，从而制造微制造加热装置，从而可以以低成本容易地制造元件，并且加热区域由 塑料薄层，使得即使在低功率下也能进行均匀的温度控制，并且各种样品可以在fa处进行热处理 速度获得反应和分析。

公开(公告)号：US07412990B2

公开(公告)日：2008-08-19

申请号：US10898964

申请日：2004-07-27

申请人： Kwang Hyo Chung ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taek Lim ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

发明人： Kwang Hyo Chung ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taek Lim ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

IPC分类号： F15C1/06

CPC分类号： B01F13/0086 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0621 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0622 ,  B01L2400/0688 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/2082 ,  Y10T137/2224

摘要： Provided is a microfluidic control device and method for controlling the microfluid, and a fine amount of fluid is controlled even with natural fluid flow and solution injection, wherein a pressure barrier of a capillary is removed by a surface tension change resulted from the solution injection to thereby obtain transport, interflow, mixing, and time delay of the microfluid, and to detail this, solution is injected to meet the boundary surface of the stopped fluid when the fluid is stopped by the stop valve, so that a function of the stop valve is removed to obtain the transport, interflow, and mixing of the fluid, and the method for controlling the microfluid may be applied to the microfluidic control device for biochemical reaction, and it uses only the capillary force change resulted from solution injection to thereby have its structure simplified.

摘要翻译： 提供了一种用于控制微流体的微流控制装置和方法，并且即使使用天然流体流动和溶液注入也能控制微量的流体，其中毛细管的压力屏障由于溶液注入导致的表面张力变化而被去除 从而获得微流体的运输，流动，混合和时间延迟，并且为了详细说明，当流体被截止阀停止时，溶液被注入以满足停止的流体的边界表面，使得截止阀 被移除以获得流体的输送，流动和混合，并且用于控制微流体的方法可以应用于用于生物化学反应的微流控制装置，并且其仅使用由溶液注入引起的毛细管力变化，从而使其 结构简化。

公开(公告)号：US07183056B2

公开(公告)日：2007-02-27

申请号：US10697295

申请日：2003-10-31

申请人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

发明人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/101

摘要： The present invention is directed to an improved multiplex PCR method for obtaining at least two PCR products from one PCR solution. In the multiplex PCR method for having at least two DNA amplified products from a sample positioned in a PCR equipment, the object of the present invention is to provide a novel multiplex PCR method characterized in that a primer annealing temperature and an extension time be changed per cycle with constant periods. When a multiplex PCR is performed in accordance with the present invention, limitations in determining PCR conditions due to a various sizes of PCR products or dimers caused by primers can be eliminated, and time and efforts required for determining the PCR conditions to various samples can also be reduced. Further, not only refined DNAs like cDNA, genomic DNA, vector, etc, but blood can be directly used as a multiplex PCR sample, and PCR amplification reaction can be performed even with a sample having the smallest amounts.

摘要翻译： 本发明涉及一种用于从一种PCR溶液获得至少两种PCR产物的改进的多重PCR方法。 本发明的目的是提供一种新的多重PCR方法，其特征在于，引物退火温度和延长时间根据 周期不变的周期。 当根据本发明进行多重PCR时，可以消除由于由引物引起的各种大小的PCR产物或二聚体导致的PCR条件的限制的限制，并且确定各种样品的PCR条件所需的时间和努力也可以 减少 此外，不仅可以将cDNA，基因组DNA，载体等精制DNA与血液直接用作多重PCR样品，即使使用量少的样品也能进行PCR扩增反应。

公开(公告)号：US06901963B2

公开(公告)日：2005-06-07

申请号：US10731211

申请日：2003-12-08

申请人： Sung Jin Kim ,  Yong Beom Shin ,  HaeSik Yang ,  Dae Sik Lee ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

发明人： Sung Jin Kim ,  Yong Beom Shin ,  HaeSik Yang ,  Dae Sik Lee ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

IPC分类号： B01J19/00 ,  G05D7/01 ,  F15B21/00

CPC分类号： G05D7/0186 ,  Y10T137/2076 ,  Y10T137/2224

摘要： Provided is a micro fluidic device that can control a flow time of micro fluid by using a capillary phenomenon. The micro fluidic device includes: a flow channel for flowing fluid, the flow channel being formed between a top substrate and a bottom substrate or between a top substrate, a bottom substrate, and a middle substrate; a flow blocking surface for stopping a flow of the fluid in the flow channel temporarily; and a hump for delaying the flow formed in the line of continuity with the flow blocking surface. The micro fluidic device of the present research can control the flow time in a simple manner.

摘要翻译： 提供了一种微流体装置，其可以通过使用毛细管现象来控制微流体的流动时间。 微流体装置包括：用于流动流体的流动通道，流动通道形成在顶部基板和底部基板之间，或者在顶部基板，底部基板和中间基板之间; 用于暂停流道中的流体流动的阻流表面; 以及用于延迟在与阻流表面连续的线中形成的流动的隆起。 本研究的微流体装置可以以简单的方式控制流动时间。

公开(公告)号：US20060127070A1

公开(公告)日：2006-06-15

申请号：US11188911

申请日：2005-07-25

申请人： Kwang Hyo Chung ,  Se Ho Park ,  Dae Sik Lee ,  Hyeon Bong Pyo ,  Chang Auck Choi

发明人： Kwang Hyo Chung ,  Se Ho Park ,  Dae Sik Lee ,  Hyeon Bong Pyo ,  Chang Auck Choi

IPC分类号： F24H1/10

CPC分类号： F24H1/162 ,  B33Y80/00

摘要： Provided is an apparatus for controlling fluid-heating using a polymer disk, which includes: a pump for discharging fluid at a uniform flow rate; a fluid-heating module including a polymer disk to convey the fluid discharged from the pump to allow a temperature of the fluid to periodically vary; and a controller for controlling the temperature of the fluid-heating module, whereby it is possible to be adapted to a fluid device for biochemical reaction such as DNA amplification, and simply used as a disposable device, which is low cost and readily manufactured in a multiple manner.

摘要翻译： 提供了一种使用聚合物盘来控制流体加热的装置，其包括：用于以均匀流量排出流体的泵; 流体加热模块，包括聚合物盘，以输送从泵排出的流体，以允许流体的温度周期性地变化; 以及用于控制流体加热模块的温度的控制器，由此可以适用于用于诸如DNA扩增的生物化学反应的流体装置，并且简单地用作一次性装置，其成本低并且易于在 多种方式。

公开(公告)号：US07398015B2

公开(公告)日：2008-07-08

申请号：US11188911

申请日：2005-07-25

申请人： Kwang Hyo Chung ,  Se Ho Park ,  Dae Sik Lee ,  Hyeon Bong Pyo ,  Chang Auck Choi

发明人： Kwang Hyo Chung ,  Se Ho Park ,  Dae Sik Lee ,  Hyeon Bong Pyo ,  Chang Auck Choi

IPC分类号： F24F1/10 ,  C12M1/00

CPC分类号： F24H1/162 ,  B33Y80/00

摘要： Provided is an apparatus for controlling fluid-heating using a polymer disk, which includes: a pump for discharging fluid at a uniform flow rate; a fluid-heating module including a polymer disk to convey the fluid discharged from the pump to allow a temperature of the fluid to periodically vary; and a controller for controlling the temperature of the fluid-heating module, whereby it is possible to be adapted to a fluid device for biochemical reaction such as DNA amplification, and simply used as a disposable device, which is low cost and readily manufactured in a multiple manner.

摘要翻译： 提供了一种使用聚合物盘来控制流体加热的装置，其包括：用于以均匀流量排出流体的泵; 流体加热模块，包括聚合物盘，以输送从泵排出的流体，以允许流体的温度周期性地变化; 以及用于控制流体加热模块的温度的控制器，由此可以适用于用于诸如DNA扩增的生物化学反应的流体装置，并且简单地用作一次性装置，其成本低并且易于在 多种方式。

公开(公告)号：US07871572B2

公开(公告)日：2011-01-18

申请号：US10944939

申请日：2004-09-21

申请人： Young Jun Kim ,  Yong Shin Kim ,  Yoon Seok Yang ,  Seung Chul Ha ,  Hae Sik Yang ,  Yun Tae Kim ,  Dae Sik Lee ,  Young Sik Hong

发明人： Young Jun Kim ,  Yong Shin Kim ,  Yoon Seok Yang ,  Seung Chul Ha ,  Hae Sik Yang ,  Yun Tae Kim ,  Dae Sik Lee ,  Young Sik Hong

IPC分类号： G01N27/04 ,  G01N27/22 ,  G01N33/00 ,  G01N33/553

CPC分类号： B82Y30/00 ,  B01J2219/005 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/0072 ,  B82Y15/00 ,  G01N27/127 ,  G01N33/4972 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/21 ,  Y10T436/212

摘要： Provided are mixed ligand metal nanoparticle chemical sensors in which metal nanoparticles are encapsulated by at least two kinds of different molecule ligands having a relatively low conductivity and various composition ratios, and a chemical sensor array in which a film of the metal nanoparticle sensor is formed on the substrate. The metal nanoparticle sensor using the mixed ligand improves sensitivity and reaction speed with respect to an analyte, and selectivity with respect to various analytes, and a kind and a composition of a ligand of the mixed ligand constituting the metal nanoparticle sensor are adjusted to allow the high sensitivity nanoparticle sensor to be applied to the sensor array technology, thereby enabling a design of sensor properties as well as systematic access to a configuration of the sensor array the most efficient for the analytes.

摘要翻译： 提供了混合的配体金属纳米颗粒化学传感器，其中金属纳米颗粒被具有相对低的导电性和各种组成比的至少两种不同的分子配体包封，以及化学传感器阵列，其中金属纳米颗粒传感器的膜形成在 底物。 使用混合配体的金属纳米颗粒传感器改善了对分析物的敏感性和反应速度，并且相对于各种分析物的选择性以及构成金属纳米颗粒传感器的混合配体的配体的种类和组成被调整，以允许 高灵敏度纳米粒子传感器应用于传感器阵列技术，从而实现传感器特性的设计，以及对分析物最有效的系统访问传感器阵列的配置。

公开(公告)号：US08585013B2

公开(公告)日：2013-11-19

申请号：US12872289

申请日：2010-08-31

申请人： Kwang Hyo Chung ,  Yo Han Choi ,  Dae Sik Lee ,  Young Jun Kim ,  Moon Youn Jung ,  Seon Hee Park

发明人： Kwang Hyo Chung ,  Yo Han Choi ,  Dae Sik Lee ,  Young Jun Kim ,  Moon Youn Jung ,  Seon Hee Park

IPC分类号： F16K31/08

CPC分类号： F16K99/0001 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0034 ,  F16K99/0046 ,  F16K2099/0078

摘要： There is provided a magnetic microvalve using a metal ball and a method of manufacturing the same. A magnetic microvalve using a metal ball according to an aspect of the invention may include: an upper substrate having a microchannel serving as a passage through which a fluid moves, a fluid inlet through which the fluid flows into the microchannel, and a fluid outlet through which the fluid, having passed through the microchannel, flows out of the microchannel; a lower substrate having a trench locally provided therein; a PDMS/metal ball combination having a metal ball and PDMS surrounding the metal ball so that the metal ball is located in a central portion thereof, the PDMS/metal ball combination being inserted into the trench provided in the lower substrate; and a magnet located above the microchannel, provided in the upper substrate, and generating magnetic force.

摘要翻译： 提供了使用金属球的磁性微型阀及其制造方法。 使用根据本发明的一个方面的金属球的磁性微型阀可以包括：具有作为流体移动通道的微通道的上基板，流体流入微通道的流体入口，以及流体出口通过 已经通过微通道的流体流出微通道; 具有局部设置在其中的沟槽的下基板; 具有围绕金属球的金属球和PDMS的PDMS /金属球组合，使得金属球位于其中心部分，PDMS /金属球组合插入设置在下基板中的沟槽中; 以及设置在上基板上的微通道上方的磁体，并产生磁力。

公开(公告)号：US08057672B2

公开(公告)日：2011-11-15

申请号：US12133295

申请日：2008-06-04

申请人： Kwang Hyo Chung ,  Yo Han Choi ,  Dae Sik Lee ,  Ju Hyun Jeon ,  Moon Youn Jung ,  Seon Hee Park

发明人： Kwang Hyo Chung ,  Yo Han Choi ,  Dae Sik Lee ,  Ju Hyun Jeon ,  Moon Youn Jung ,  Seon Hee Park

IPC分类号： B01D69/06 ,  B01D63/08 ,  B01D29/00

CPC分类号： G01N33/491 ,  B01L3/502753 ,  B01L2300/043 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B33Y80/00

摘要： Provided is a disposable multi-layered filtration device for the separation of blood plasma which can be applied to a biochip and appropriate for disposal uses. The filtration device for the separation of blood plasma includes: an upper substrate including a blood inlet; an intermediate substrate including a filtering unit for extracting blood plasma from blood flowing through the blood inlet; and a lower substrate including an air outlet, wherein the upper substrate, the intermediate substrate, and the lower substrate are stacked and adhered.

摘要翻译： 提供了一种用于分离血浆的一次性多层过滤装置，其可以应用于生物芯片并适合于处置用途。 用于分离血浆的过滤装置包括：包括血液入口的上基板; 中间基板，包括用于从流过血液入口的血液中提取血浆的过滤单元; 以及包括空气出口的下基板，其中所述上基板，所述中间基板和所述下基板被堆叠和粘附。