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公开(公告)号：US07588322B2
公开(公告)日：2009-09-15
申请号：US11669608
申请日：2007-01-31
IPC分类号： B41J2/045
CPC分类号： B41J2/14209 , B41J2002/14217 , B41J2202/11
摘要： A liquid droplet discharging piezoelectric device 1 provided with a cavity member 11 with a built-in cavity 3; an introduction member 13 having introduction channel 5 connecting with the cavity 3; and a nozzle member 12 having nozzle channel 4 connecting with the cavity 3 on a side opposite to the channel 5. This liquid droplet discharging piezoelectric device 1 is provided with an introduction port 6, attached to the introduction member 13, capable of introducing a liquid into the cavity 3 via the introduction channel 5, and a discharge port 7, attached to the nozzle member 12, capable of discharging as droplets a liquid filled in the cavity 3 via the nozzle channel 40. Even in a case where an amount of liquid droplets is of a nanoliter (nl) order, excellent stability and reproducibility are attained, and the unit can stably be operated when attached to an apparatus.
摘要翻译： 具有内置空腔3的空腔构件11的液滴排出压电装置1; 引入构件13，其具有与空腔3连接的引入通道5; 以及喷嘴构件12，其具有在与通道5相反的一侧与空腔3连接的喷嘴通道4.该液滴排出压电装置1设置有引入口6，引入口6安装在导入构件13上，能够引入液体 通过引入通道5进入空腔3和附接到喷嘴构件12的排出口7，其能够通过喷嘴通道40作为液滴喷射填充在空腔3中的液体。即使在液体量 液滴具有纳升级（nl）级，达到优异的稳定性和再现性，并且当装置安装时该装置可以稳定地操作。
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公开(公告)号：US20070120899A1
公开(公告)日：2007-05-31
申请号：US11669608
申请日：2007-01-31
IPC分类号： B41J2/045
CPC分类号： B41J2/14209 , B41J2002/14217 , B41J2202/11
摘要： A liquid droplet discharging piezoelectric device 1 provided with a cavity member 11 with a built-in cavity 3; an introduction member 13 having introduction channel 5 connecting with the cavity 3; and a nozzle member 12 having nozzle channel 4 connecting with the cavity 3 on a side opposite to the channel 5. This liquid droplet discharging piezoelectric device 1 is provided with an introduction port 6, attached to the introduction member 13, capable of introducing a liquid into the cavity 3 via the introduction channel 5, and a discharge port 7, attached to the nozzle member 12, capable of discharging as droplets a liquid filled in the cavity 3 via the nozzle channel 40. Even in a case where an amount of liquid droplets is of a nanoliter (nl) order, excellent stability and reproducibility are attained, and the unit can stably be operated when attached to an apparatus.
摘要翻译： 具有内置空腔3的空腔构件11的液滴排出压电装置1; 引入构件13，其具有与空腔3连接的引入通道5; 以及喷嘴构件12，其具有与通道5相对的一侧与空腔3连接的喷嘴通道4。 该液滴排出压电元件1设置有导入口6，安装在导入部件13上，能够经由导入流路5将液体引入空腔3，以及安装在喷嘴构件12上的排出口7， 能够经由喷嘴通道40作为液体喷射填充在空腔3中的液体。 即使在液滴量为纳升（nl）次的情况下也能获得良好的稳定性和再现性，并且在连接到设备时可以稳定地操作该单元。
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公开(公告)号：US06899283B2
公开(公告)日：2005-05-31
申请号：US10252735
申请日：2002-09-23
申请人： Takao Ohnishi , Toshikazu Hirota , Yoshihiro Iseki
发明人： Takao Ohnishi , Toshikazu Hirota , Yoshihiro Iseki
IPC分类号： G01N33/53 , B01J19/00 , B01L3/02 , B05B12/10 , B05B17/06 , B41J2/045 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B40/02 , C40B50/06 , G01N1/00 , G01N35/10 , G01N37/00 , B05B3/04
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725 , B01L3/0268 , B01L2200/143 , B01L2400/0439 , B05B12/10 , B05B17/0607 , B41J2/04571 , B41J2/04581 , B41J2/04588 , B41J2/04595
摘要： A liquid droplet ejection method and apparatus comprising a base member and a piezoelectric/electrostrictive element. The interior of the base member includes a pressurizing chamber and an ejection opening. When the interval from the last pressurizing operation to the current pressurizing operation exceeds a predetermined time, applied voltage to the piezoelectric/electrostrictive element is increased at a first increase rate so that liquid within the pressurizing chamber is pressurized for ejection at a first pressurizing increase rate. When the interval from the last pressurizing operation to the current pressurizing operation is shorter than the predetermined time, the applied voltage is increased at a second increase rate smaller than the first increase rate so that the liquid is pressurized for ejection at a second pressurizing rate which is relatively small. This ensures proper pressurization of a liquid whose viscosity increases over time, achieving proper ejections.
摘要翻译： 一种液滴喷射方法和装置，包括基体和压电/电致伸缩元件。 基部构件的内部包括加压室和喷射口。 当从最后一个加压操作到当前加压操作的间隔超过预定时间时，施加到压电/电致伸缩元件的电压以第一增加速率增加，使得加压室内的液体以第一增压速率被加压以喷射 。 当从最后一个加压操作到当前加压操作的间隔小于预定时间时，施加电压以比第一增加速率小的第二增加速率增加，使得液体以第二加压速率加压以喷射， 比较小 这确保了粘度随时间增加的液体的适当加压，实现适当的喷射。
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公开(公告)号：US06752326B2
公开(公告)日：2004-06-22
申请号：US09884457
申请日：2001-06-19
申请人： Toshikazu Hirota , Takao Ohnishi
发明人： Toshikazu Hirota , Takao Ohnishi
IPC分类号： B05B108
CPC分类号： F02M61/1846 , B05B17/0607 , F02M51/04 , F02M51/0603 , F02M57/021 , F02M57/027 , F02M61/166 , F02M61/168 , F02M61/18 , F02M61/1833 , F02M61/186 , F02M2200/90 , F02M2200/9007 , F02M2200/9015 , F02M2200/9038 , F02M2200/9046
摘要： The liquid droplet ejection apparatus includes a liquid supply path, a plurality of mutually independent pressurizing chambers, a plurality of liquid introduction bores for establishing communication between the corresponding pressurizing chambers and the liquid supply path, and a plurality of ejection nozzles for establishing communication between the corresponding pressurizing chambers and the exterior of the liquid droplet ejection apparatus. An ejection bore formed at the end portion of the ejection nozzle has a hollow cylindrical form and the inside diameter thereof increases toward an ejection opening. When a potential difference is applied between two electrodes of a piezoelectric/electrostrictive element, a ceramic sheet forming the upper wall of the pressurizing chamber deforms to thereby cause a change of the volume of the pressurizing chamber. Thus, liquid pressure within the pressurizing chamber increases to thereby cause simultaneous ejection of a plurality of liquid droplets from the ejection opening.
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公开(公告)号：US06465190B1
公开(公告)日：2002-10-15
申请号：US09694135
申请日：2000-10-23
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译： 公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤， 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。
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公开(公告)号：US07160512B2
公开(公告)日：2007-01-09
申请号：US10135200
申请日：2002-04-30
申请人： Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
发明人： Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
IPC分类号： B01L3/02
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00691 , B01J2219/00693 , B01J2219/00695 , B01J2219/00702 , B01J2219/00722 , B01L3/0241 , C40B40/06 , C40B60/14 , G01N35/109 , Y10T436/2575
摘要： A biochip manufacturing method can densely align spots of a plurality of samples in predetermined locations on a substrate using a discharge head equipped with one or more discharge modules composed of one or more discharge units, and a first moving table on which one or more trays with one or more substrates fixed thereon are detachably mounted. According to the biochip manufacturing method, the high precision in the operation for densely aligning and fixing droplets with a minute volume on a predetermined substrate (micro spot-forming operation) can be attained, and the time required for the micro spot-forming operation can be shortened as well.
摘要翻译： 生物芯片制造方法可以使用配备有由一个或多个排出单元组成的一个或多个排出模块的排出头以及第一移动工作台，将一个或多个具有 固定在其上的一个或多个基板可拆卸地安装。 根据生物芯片的制造方法，可以实现在规定的基板上微小对准和固定微小的液滴的操作的高精度（微点形成操作），并且微点成形操作所需的时间可以 也缩短了。
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公开(公告)号：US06875402B2
公开(公告)日：2005-04-05
申请号：US09977567
申请日：2001-10-15
申请人： Toshikazu Hirota , Takao Ohnishi
发明人： Toshikazu Hirota , Takao Ohnishi
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N2035/1041 , Y10S73/04 , Y10T436/2575
摘要： A micropipette usable for producing a biochip inclusive of DNA micro arrays capable of arraying and fixing droplets of a micro-volume on a substrate in a high density includes a main body, at least one cavity for storing a sample, at least one ejection port, a piezoelectic/electrostrictive element mounted on the outer surface of the main body, and at least one sample inlet port for supplying sample from the outside. A sample that is temporarily stored in the cavity is discharged from at least one ejection port, by virtue of the movement of the piezoelectric/electrostrictive element, to outside of the micropipette via a through hole in a nozzle portion formed in the pipette main body. The nozzle portion through hole has three or more projection radially protruding from the center of the through hole and having a specific shape.
摘要翻译： 可用于生产包括能够以高密度排列和固定微量体积的液滴的DNA微阵列的生物芯片的微量移液器包括主体，至少一个用于存储样品的空腔，至少一个排出口， 安装在主体的外表面上的压电/电致伸缩元件和用于从外部供应样品的至少一个样品入口。 通过压电/电致伸缩元件的移动，临时存储在空腔中的样品通过形成在移液管主体中的喷嘴部分中的通孔从至少一个喷射口排出到微量移液管外部。 喷嘴部通孔具有从通孔的中心径向突出并具有特定形状的三个以上的突起。
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公开(公告)号：US06753144B1
公开(公告)日：2004-06-22
申请号：US09868832
申请日：2001-06-21
申请人： Toshikazu Hirota , Takao Ohnishi
发明人： Toshikazu Hirota , Takao Ohnishi
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0241 , B01L3/0268 , B01L3/5085 , B01L3/5088 , B01L9/54 , B01L2200/021 , B01L2200/027 , B01L2300/0816 , B01L2300/0819 , B01L2400/027 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , Y10T436/25 , Y10T436/2575 , C12Q2565/507
摘要： When genetic analyses are performed using the DNA microarray of the present invention, the inspection accuracy is improved. A sample solution is supplied onto a base plate 10 to prepare the DNA microarray 20 which includes a large number of spots 80 containing capture solutions arranged on the base plate 10. The capture solutions are adapted to specifically react with a specimen and provide information about a structure within the specimen. In the microarray 20, the planar configuration of the spots 80 are substantially circular, and a plurality of spots having different spot sizes are formed on the base plate.
摘要翻译： 当使用本发明的DNA微阵列进行遗传分析时，提高了检查精度。 将样品溶液供应到基板10上以制备包括大量含有布置在基板10上的捕获溶液的斑点80的DNA微阵列20.捕获溶液适于与样品特异性反应并提供关于 标本内的结构。 在微阵列20中，斑点80的平面构造基本上是圆形的，并且在基板上形成具有不同光斑尺寸的多个斑点。
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公开(公告)号：US06649343B1
公开(公告)日：2003-11-18
申请号：US09693228
申请日：2000-10-20
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/00237 , G01N2035/1039 , C12Q2565/507
摘要： A DNA chip includes a large number of minute spots formed by dripping sample solutions onto a base plate. The base plate is provided with positional deviation-correcting means for displacing the spots and automatically correcting any positional deviation of each of the spots. The positional deviation-correcting means includes any one of at least one projection, at least one recess and electric field-generating means for providing charged states on the base plate centered at a correct position for each of the spots supplied on the base plate.
摘要翻译： DNA芯片包括通过将样品溶液滴落到基板上而形成的大量微小斑点。 基板设置有用于移动斑点并自动校正每个斑点的任何位置偏差的位置偏差校正装置。 位置偏差校正装置包括至少一个突起，至少一个凹部和电场产生装置中的任何一个，用于在基板上以提供在基板上的每个点的正确位置为中心提供充电状态。
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公开(公告)号：US06508411B1
公开(公告)日：2003-01-21
申请号：US09701552
申请日：2001-01-08
申请人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
发明人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
IPC分类号： B05B1704
CPC分类号： B41J2/04541 , B41J2/04581 , B41J2/04588 , B41J2/14233 , B41J2/14274 , F04B43/0081 , F04B43/046
摘要： A liquid-drop spraying device is provided which can perform a stable liquid discharge without producing air bubbles in the liquid of the pressurized room as well as the amount of liquid supply per unit time is increased. The liquid can be flowed into the pressurized room uniformly and without entrainment of bubbles from the nozzle side by making an initial discharge (or charge) time constant larger than a subsequent discharge (or charge) time constant, which in turn allows liquid in the pressurized room to be replaced by slow suction followed by fast suction.
摘要翻译： 提供一种液滴喷射装置，其能够在不会在加压室的液体中产生气泡的情况下进行稳定的液体排出，并且每单位时间的液体供应量增加。 通过使初始放电（或充电）时间常数大于随后的放电（或充电）时间常数，液体可以均匀地流入加压室并且不会从喷嘴侧夹带气泡，这又导致加压的液体 房间被缓慢的吸力替代，然后快速吸入。
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