公开(公告)号：US20080292655A1

公开(公告)日：2008-11-27

申请号：US12111305

申请日：2008-04-29

申请人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Silvia Perri ,  Timothy C. Fong

发明人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Silvia Perri ,  Timothy C. Fong

IPC分类号： A61K39/00 ,  A61P37/00

CPC分类号： C12N15/79

摘要： Expression cassettes are provided comprising a promoter operably linked to a nucleic acid molecule which, when transcribed in vivo, forms double-stranded RNA that induces the production of interferon. Expression cassettes also are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, forms a ribozyme or antisense RNA molecule that stimulates an immune response. In addition, expression cassettes are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, stimulates apoptosis. Finally, gene delivery vectors are provided which contain such expression cassettes, host cells containing the gene delivery vectors, and methods of utilizing the expression cassettes, gene delivery vectors, and host cells.

摘要翻译： 提供了表达盒，其包括可操作地连接到核酸分子的启动子，其在体内转录时形成诱导产生干扰素的双链RNA。 还提供了包含与核酶或反义核酸分子可操作地连接的启动子的表达盒，其在体内转录时形成刺激免疫应答的核酶或反义RNA分子。 此外，提供了包含可操作地连接到核酶或反义核酸分子的启动子的表达盒，其在体内转录时刺激细胞凋亡。 最后，提供含有这种表达盒的基因递送载体，含有基因递送载体的宿主细胞，以及利用表达盒，基因递送载体和宿主细胞的方法。

公开(公告)号：US5843723A

公开(公告)日：1998-12-01

申请号：US739167

申请日：1996-10-30

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12N5/10

CPC分类号： C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present invention provides compositions and method,, for utilizing recombinant alphavirus vectors.

摘要翻译： 本发明提供了利用重组甲病毒载体的组合物和方法。

公开(公告)号：US20100330121A1

公开(公告)日：2010-12-30

申请号：US12845516

申请日：2010-07-28

申请人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Sergey A. Dryga ,  Sondra Schlessinger ,  Ilya Frolov

发明人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Barbara A. Belli ,  Sergey A. Dryga ,  Sondra Schlessinger ,  Ilya Frolov

IPC分类号： A61K39/12 ,  C12N15/63 ,  A61P37/04

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US07811812B2

公开(公告)日：2010-10-12

申请号：US10391441

申请日：2003-03-17

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Drvga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Drvga ,  Ilya Frolov

IPC分类号： C12N15/63

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US07572628B2

公开(公告)日：2009-08-11

申请号：US10346880

申请日：2003-01-16

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC分类号： C12N15/63 ,  C12N15/85 ,  A61K48/00 ,  C07H21/04

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

摘要翻译： 本发明提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US20100173412A1

公开(公告)日：2010-07-08

申请号：US12538494

申请日：2009-08-10

申请人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M.W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

发明人： Thomas W. Dubensky, JR. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M.W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC分类号： C12N5/071

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present disclosure provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems

摘要翻译： 本公开提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法

公开(公告)号：US06451592B1

公开(公告)日：2002-09-17

申请号：US08944465

申请日：1997-10-06

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C12N1500

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

公开(公告)号：US06465634B1

公开(公告)日：2002-10-15

申请号：US09415900

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C07H2104

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

公开(公告)号：US06458560B1

公开(公告)日：2002-10-01

申请号：US09415868

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilva Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilva Frolov

IPC分类号： C12P2106

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US06391632B1

公开(公告)日：2002-05-21

申请号：US09415784

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C12N510

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as is compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质，与野生型重组甲病毒颗粒相比。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。