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1.发明授权Hydrolases, nucleic acids encoding them and methods for making and using them 有权水解酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US09193962B2
公开(公告)日：2015-11-24
申请号：US13619974
申请日：2012-09-14
IPC分类号： C07H21/04 , C12N9/14 , C12N9/16 , C12N9/20 , A23C9/20 , A23C19/05 , A23C19/06 , A23K1/165 , A23L1/03 , A61K8/02 , A61K8/37 , A61K8/60 , A61K8/66 , A61Q5/06 , A61Q11/00 , A61Q19/00 , C10M129/70 , C10M129/76 , C11C3/08 , C11D3/386 , C12C1/047 , C12C5/00 , C12C11/00 , C12N9/18 , C12Q1/34 , D06M16/00 , G01N33/573
CPC分类号： C12N9/20 , A23C9/20 , A23C19/05 , A23C19/063 , A23K10/14 , A23K20/189 , A23L29/06 , A23V2002/00 , A61K8/0216 , A61K8/375 , A61K8/606 , A61K8/66 , A61K38/465 , A61K2800/86 , A61Q5/06 , A61Q11/00 , A61Q19/00 , C10M129/70 , C10M129/76 , C10M2207/281 , C10M2207/289 , C10M2207/40 , C11C3/08 , C11D3/386 , C11D3/38627 , C11D3/38636 , C12C1/047 , C12C5/004 , C12C11/003 , C12N9/14 , C12N9/16 , C12N9/18 , C12P7/6418 , C12Q1/34 , C12Y301/01003 , D06M16/003 , D06M2200/12 , D06M2200/30 , D06M2200/50 , G01N33/573 , G01N2500/02
摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
摘要翻译： 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括耐热和耐热水解酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。
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2.发明授权Hydrolases, nucleic acids encoding them and methods for making and using them 有权水解酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US08298799B2
公开(公告)日：2012-10-30
申请号：US10547956
申请日：2004-03-08
IPC分类号： C12N9/14
CPC分类号： C12N9/20 , A23C9/20 , A23C19/05 , A23C19/063 , A23K10/14 , A23K20/189 , A23L29/06 , A23V2002/00 , A61K8/0216 , A61K8/375 , A61K8/606 , A61K8/66 , A61K38/465 , A61K2800/86 , A61Q5/06 , A61Q11/00 , A61Q19/00 , C10M129/70 , C10M129/76 , C10M2207/281 , C10M2207/289 , C10M2207/40 , C11C3/08 , C11D3/386 , C11D3/38627 , C11D3/38636 , C12C1/047 , C12C5/004 , C12C11/003 , C12N9/14 , C12N9/16 , C12N9/18 , C12P7/6418 , C12Q1/34 , C12Y301/01003 , D06M16/003 , D06M2200/12 , D06M2200/30 , D06M2200/50 , G01N33/573 , G01N2500/02
摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphlipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzyme, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
摘要翻译： 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括热稳定和耐热水解酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。
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3.发明申请Hydrolases, Nucleic Acids Encoding Them And Methods For Making And Using Them 有权水解酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20070202566A1
公开(公告)日：2007-08-30
申请号：US10547956
申请日：2004-03-08
CPC分类号： C12N9/20 , A23C9/20 , A23C19/05 , A23C19/063 , A23K10/14 , A23K20/189 , A23L29/06 , A23V2002/00 , A61K8/0216 , A61K8/375 , A61K8/606 , A61K8/66 , A61K38/465 , A61K2800/86 , A61Q5/06 , A61Q11/00 , A61Q19/00 , C10M129/70 , C10M129/76 , C10M2207/281 , C10M2207/289 , C10M2207/40 , C11C3/08 , C11D3/386 , C11D3/38627 , C11D3/38636 , C12C1/047 , C12C5/004 , C12C11/003 , C12N9/14 , C12N9/16 , C12N9/18 , C12P7/6418 , C12Q1/34 , C12Y301/01003 , D06M16/003 , D06M2200/12 , D06M2200/30 , D06M2200/50 , G01N33/573 , G01N2500/02
摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphlipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzyme, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
摘要翻译： 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括热稳定和耐热水解酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。
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4.发明授权Hydrolases, nucleic acids encoding them and methods to produce triglycerides 有权水解酶，编码它们的核酸和产生甘油三酯的方法公开(公告)号：US08313918B2
公开(公告)日：2012-11-20
申请号：US13083461
申请日：2011-04-08
申请人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
发明人： Christopher L. G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
IPC分类号： C12Q1/34 , C12Q1/00 , C12N9/14 , C12N1/20 , C12N15/00 , C12P7/00 , C12P21/04 , C12P21/06 , C07H21/04
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。
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5.发明申请HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS TO PRODUCE TRIGLYCERIDES 有权水解物，核酸编码方法及生产TRIGLYCERIDES的方法公开(公告)号：US20110287136A1
公开(公告)日：2011-11-24
申请号：US13083472
申请日：2011-04-08
申请人： Christopher L.G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
发明人： Christopher L.G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。
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公开(公告)号：US20100129882A1
公开(公告)日：2010-05-27
申请号：US12550300
申请日：2009-08-28
申请人： Christopher L.G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
发明人： Christopher L.G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
IPC分类号： C12P7/64
CPC分类号： C12P7/6454 , C12N9/14 , C12N9/20 , C12P7/6418 , C12P7/6445
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
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公开(公告)号：US09045712B2
公开(公告)日：2015-06-02
申请号：US13502105
申请日：2010-10-08
申请人： Christopher L. G. Dayton , Flavio Da Silva Galhardo , Nelson Barton , Tim Hitchman , Jonathan Lyon , Eileen O'Donoghue , Mark A. Wall
发明人： Christopher L. G. Dayton , Flavio Da Silva Galhardo , Nelson Barton , Tim Hitchman , Jonathan Lyon , Eileen O'Donoghue , Mark A. Wall
CPC分类号： C11B3/003 , C10G2300/1011 , C10L1/026 , C11B3/04 , C12N9/88 , C12P7/18 , C12P7/6445 , C12P7/6481 , C12Y406/01013 , Y02E50/13 , Y02P20/52 , Y02P30/20
摘要： In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.
摘要翻译： 在替代实施方案中，本发明提供磷脂酰肌醇特异性磷脂酶C（PI-PLC）酶，编码它们的核酸，与其特异性结合的抗体，以及制备和使用它们的方法。 还提供了包括使用这些磷脂酶的工业方法和产品。 在某些实施方案中，本文提供了用于在脂质基质内水合非水合磷脂（NHP）的方法。 这些方法使NHP迁移到油 - 水界面，从而允许NHP从脂质中反应和/或除去。 在某些实施方案中，提供了从植物油中除去NHP，可水合磷脂和卵磷脂以产生可用于食品生产和/或非食品应用的脱胶油或脂肪产品的方法。 在某些实施方案中，本文提供了用于NHP水合的方法，然后进行酶处理和除去各种磷脂和卵磷脂。 本文提供的方法可以在粗脱水油或水脱胶油上实施。
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8.发明授权Hydrolases, nucleic acids encoding them and methods for making and using them 有权水解酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US08465942B2
公开(公告)日：2013-06-18
申请号：US13527333
申请日：2012-06-19
申请人： Christopher L. G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
发明人： Christopher L. G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
IPC分类号： C12Q1/34 , C12Q1/00 , C12N9/14 , C12N1/20 , C12N15/00 , C12P7/00 , C12P21/04 , C12P21/06 , C07H21/04
CPC分类号： C12P7/6454 , C12N9/14 , C12N9/20 , C12P7/6418 , C12P7/6445
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。
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公开(公告)号：US08357503B2
公开(公告)日：2013-01-22
申请号：US12550300
申请日：2009-08-28
申请人： Christopher L. G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
发明人： Christopher L. G. Dayton , Nelson Barton , Analia Bueno , Joslin G. Cuenca , Tim Hitchman , Katie A. Kline , Jonathan Lyon , Mark L. Miller , Mark A. Wall
IPC分类号： C12Q1/34 , C12Q1/00 , C12N9/14 , C12N1/20 , C12N15/00 , C12P7/00 , C12P21/04 , C12P21/06 , C07H21/04
CPC分类号： C12P7/6454 , C12N9/14 , C12N9/20 , C12P7/6418 , C12P7/6445
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
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10.发明申请HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR BIOCATALYTIC SYNTHESIS OF STRUCTURED LIPIDS 有权水解物，核酸编码方法及结构化脂肪的生物合成方法公开(公告)号：US20120276618A1
公开(公告)日：2012-11-01
申请号：US13083480
申请日：2011-04-08
申请人： Christopher L.G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
发明人： Christopher L.G. Dayton , Tim Hitchman , Katie Kline , Jonathan Lyon , Mark A. Wall , Nelson R. Barton
IPC分类号： C11C1/04
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。
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