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公开(公告)号：US06368807B2
公开(公告)日：2002-04-09
申请号：US09733365
申请日：2000-12-08
IPC分类号： C12Q168
CPC分类号： C07F17/02
摘要： A compound of the following formula: Ea—La—X—Lb—Eb in which each of Ea and Eb independently is a group having oxidation-reduction activity and having a conjugated system in its group; X is a divalent cyclic group; and each of La and Lb independently is a group which does not form a conjugated system in combination with the conjugated system of each of Ea and Eb and at least one of which has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound, is favorably employable as an electroconductive threading intercalator in an electrochemical method for detecting complementary DNA fragments.
摘要翻译： 下式的化合物：其中Ea和Eb各自独立地是具有氧化还原活性的基团并且在其组中具有共轭体系; X是二价环状基团; 独立地，La和Lb各自是不与Ea和Eb各自的共轭体系结合形成共轭体系的基团，并且其中至少一个具有赋予化合物或可转化位点水溶性的位点 成为赋予化合物水溶性的部位有利地用作检测互补DNA片段的电化学方法中的导电穿线嵌入剂。
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2.发明授权公开(公告)号：US06399305B1
公开(公告)日：2002-06-04
申请号：US09588950
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , Y10T436/143333 , C12Q2563/173 , C12Q2563/113
摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.
摘要翻译： 分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液 与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。
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公开(公告)号：US06864055B2
公开(公告)日：2005-03-08
申请号：US09887625
申请日：2001-06-22
申请人： Yoshihiko Makino , Yoshihiko Abe , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
发明人： Yoshihiko Makino , Yoshihiko Abe , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
IPC分类号： G01N33/53 , C07B61/00 , C07K14/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N27/416 , G01N33/566 , B01D57/02 , C12P19/34 , G01N30/02
CPC分类号： C12Q1/6825 , C07K14/003 , C12Q1/6837 , C40B40/00 , C12Q2565/501 , C12Q2565/102 , C12Q2565/607
摘要： A method of detecting nucleic acid fragments in plural samples is performed by the steps of: attaching an electroconductive label to nucleic acid fragments in one sample and attaching a different electroconductive label to nucleic acid fragments in another sample; preparing a mixture of these samples; spotting the mixture on an electroconductive microarray having plural electrodes onto which probe molecules complementary to the nucleic acid fragments are fixed, so that hybridization between the nucleic acid fragments and the probe molecules on the electroconductive microarray can proceed to form hybrid structures; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the former label and detecting on the electrode an electric current; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the latter label and detecting on the electrode an electric current; and comparing the electric current detected in the former detecting procedure and that detected in the latter detecting procedure.
摘要翻译： 通过以下步骤进行检测多个样品中的核酸片段的方法：将导电标记物附着在一个样品中的核酸片段上，并将不同的导电标记连接到另一个样品中的核酸片段; 准备这些样品的混合物; 在具有多个电极的导电微阵列上将该混合物点样，在该导电微阵列上固定有与核酸片段互补的探针分子，使得导电微阵列上的核酸片段和探针分子之间的杂交可以进行以形成杂化结构; 向所述电极施加与所述前标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 向所述电极施加与后一标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 并比较前一检测程序检测的电流和后一检测程序中检测的电流。
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公开(公告)号：US06713255B1
公开(公告)日：2004-03-30
申请号：US09589221
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C07K14/003 , B01J19/0046 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , B01J2219/00729 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B40/06 , C12Q2565/607
摘要： A DNA chip (or PNA chip) composed of a solid carrier and plural DNA fragments (or PNA fragments) fixed onto is the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed at each another end onto a surface of the solid carrier having no DNA fragments (or no PNA fragments) on its surface is effective for high sensitive quantitative analysis of a nucleic acid fragment complementary to the DNA fragment (or PNA fragment).
摘要翻译： 由固体载体和固定在其上的多个DNA片段（或PNA片段）组成的DNA芯片（或PNA芯片）是每一端的固体载体，其中在每一端具有亲水部分的多个短链间隔基分子是 固定在其表面上没有DNA片段（或没有PNA片段）的固体载体的表面上对于与DNA片段（或PNA片段）互补的核酸片段的高敏感性定量分析是有效的。
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公开(公告)号：US06506567B2
公开(公告)日：2003-01-14
申请号：US09774895
申请日：2001-01-31
IPC分类号： C12Q168
CPC分类号： C07D471/06 , C12Q1/6816 , Y10T436/143333 , C12Q2563/173
摘要： A water-soluble fluorescent intercalator compound having the formula: F—La—X F is a fluorescent moiety, X is a divalent cyclic group, and La is a linking group, and at least one of X and La has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound is favorably employable as a fluorescent intercalator in a method for fluorometrically detecting complementary DNA fragments.
摘要翻译： 具有式：F的水溶性荧光嵌入剂化合物是荧光部分，X是二价环状基团，La是连接基团，X和La中的至少一个具有赋予化合物水溶性的部位或 可以转化为赋予化合物水溶性的部位的部位在荧光测定互补DNA片段的方法中有利地用作荧光嵌入剂。
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公开(公告)号：US20060147991A1
公开(公告)日：2006-07-06
申请号：US11377058
申请日：2006-03-16
申请人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
发明人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00497 , B01J2219/00527 , B01J2219/00533 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07C317/08 , C07H21/00 , C40B40/00
摘要： A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.
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7.发明申请Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒公开(公告)号：US20050266450A1
公开(公告)日：2005-12-01
申请号：US11106612
申请日：2005-04-15
CPC分类号： G01N33/523 , C12Q1/42 , C12Q1/6816 , C12Q1/6869 , C12Q2565/625 , C12Q2565/301 , C12Q2521/543 , C12Q2533/101
摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.
摘要翻译： 本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的 用于定量焦磷酸的分析元素。 本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。
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8.发明授权Method of stably producing microporous membrane and use thereof in method of separating and purifying nucleic acid 有权稳定生产微孔膜的方法及其在核酸分离纯化方法中的应用公开(公告)号：US08511482B2
公开(公告)日：2013-08-20
申请号：US11630135
申请日：2005-09-15
申请人： Hideaki Tanaka , Yoshihiko Makino
发明人： Hideaki Tanaka , Yoshihiko Makino
CPC分类号： B01D67/0013 , B01D15/00 , B01D15/08 , B01D67/0016 , B01D67/0093 , B01D71/12 , B01D2323/08 , B01D2323/22 , B01D2325/02 , B01D2325/022 , B01D2325/12 , B01J20/26 , B01J20/265 , B01J20/28033 , C08J9/28
摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.
摘要翻译： 一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑 聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化 的铸造表面保持在±3.0℃
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公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
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10.发明申请公开(公告)号：US20050277152A1
公开(公告)日：2005-12-15
申请号：US11155469
申请日：2005-06-20
申请人： Yoshihiko Makino , Toshihiro Mori
发明人： Yoshihiko Makino , Toshihiro Mori
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2565/137
摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
摘要翻译： 本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以 自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。 本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中 具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。
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