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    CYTOPLASMIC TRANSFER TO DE-DIFFERENTIATE RECIPIENT CELLS
    3.
    发明申请
    CYTOPLASMIC TRANSFER TO DE-DIFFERENTIATE RECIPIENT CELLS 审中-公开
    细胞色素转移以分化受体细胞

    公开(公告)号：US20150132853A1

    公开(公告)日：2015-05-14

    申请号：US14302384

    申请日：2014-06-11

    申请人： Advanced Cell Technology, Inc.

    发明人： Karen B. Chapman

    IPC分类号： C12N5/0735 ,  C12N5/071

    CPC分类号： C12N5/0606 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0602 ,  C12N5/0696 ,  C12N5/16 ,  C12N9/1241 ,  C12N15/79 ,  C12N15/873 ,  C12N2501/00 ,  C12N2503/00 ,  C12N2506/00 ,  C12N2510/04 ,  C12N2517/10

    摘要： Methods for de-differentiating or altering the life-span of desired “recipient” cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and/or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods.

    摘要翻译： 提供了通过从更原始，较不分化的细胞类型例如卵母细胞或卵裂球引入细胞质来去分化或改变所需“受体”细胞例如人体细胞的寿命的方法。 这些方法可以用于产生胚胎干细胞，并且通过允许所需细胞经受多种遗传修饰而不变老，提高基因治疗的效率。 这样的细胞质可以被分馏和/或进行消减杂交，并通过重组方法鉴定和产生活性物质（足以去分化）。

    METHODS OF PRODUCING RPE CELLS AND COMPOSITIONS OF RPE CELLS
    4.
    发明申请
    METHODS OF PRODUCING RPE CELLS AND COMPOSITIONS OF RPE CELLS 审中-公开
    RPE细胞的生产方法和RPE细胞的组成

    公开(公告)号：US20150086512A1

    公开(公告)日：2015-03-26

    申请号：US14254833

    申请日：2014-04-16

    申请人： Advanced Cell Technology, Inc.

    发明人： Christopher Malcuit ,  Linda Lemieux ,  William Holmes ,  Pedro Huertas ,  Lucy Vilner

    IPC分类号： C12N5/079 ,  A61K35/30

    CPC分类号： C12N5/0621 ,  A61K35/12 ,  A61K35/30 ,  C12N2501/10 ,  C12N2501/734 ,  C12N2506/02 ,  C12N2509/00 ,  C12N2533/54

    摘要： The present invention provides improved methods for producing RPE cells from human embryonic stem cells or from other human pluripotent stem cells. The invention also relates to human retinal pigmented epithelial cells derived from human embryonic stem cells or other human multipotent or pluripotent stem cells. hRPE cells derived from embryonic stem cells are molecularly distinct from adult and fetal-derived RPE cells, and are also distinct from embryonic stem cells. The hRPE cells described herein are useful for treating retinal degenerative diseases.

    摘要翻译： 本发明提供了用于从人胚胎干细胞或其他人多能干细胞产生RPE细胞的改进方法。 本发明还涉及源自人胚胎干细胞或其他人多能或多能干细胞的人视网膜色素上皮细胞。 衍生自胚胎干细胞的hRPE细胞与成体和胎儿衍生的RPE细胞分子不同，并且也不同于胚胎干细胞。 本文所述的hRPE细胞可用于治疗视网膜退行性疾病。

    DERIVATION OF EMBRYONIC STEM CELLS
    5.
    发明申请
    DERIVATION OF EMBRYONIC STEM CELLS 有权
    胚胎干细胞的衍生

    公开(公告)号：US20140377865A1

    公开(公告)日：2014-12-25

    申请号：US14154163

    申请日：2014-01-13

    申请人： ADVANCED CELL TECHNOLOGY, INC.

    发明人： ROBERT P. LANZA ,  YOUNG GIE CHUNG

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0606 ,  A61K35/545 ,  C12N5/0604 ,  C12N15/873 ,  C12N2501/119 ,  C12N2501/603 ,  C12N2501/91 ,  C12N2502/02 ,  C12N2506/02

    摘要： This present invention provides novel methods for deriving embryonic stem cells, those cells and cell lines, and the use of the cells for therapeutic and research purposes without the destruction of the embryo. It also relates to novel methods of establishing and storing an autologous stem cell line prior to implantation of an embryo, e.g., in conjunction with reproductive therapies such as IVF.

    摘要翻译： 本发明提供用于衍生胚胎干细胞，那些细胞和细胞系的新颖方法，以及用于治疗和研究目的而不破坏胚胎的细胞的用途。 它还涉及在植入胚胎之前建立和储存自体干细胞系的新方法，例如与生殖治疗如IVF结合。

    Method of differentiation of morula or inner cell mass cells and method of making lineage-defective embryonic stem cells
    6.
    发明授权
    Method of differentiation of morula or inner cell mass cells and method of making lineage-defective embryonic stem cells 有权
    桑ula或内细胞群细胞的分化方法及其谱系缺陷胚胎干细胞的制备方法

    公开(公告)号：US08753884B2

    公开(公告)日：2014-06-17

    申请号：US12152779

    申请日：2008-05-16

    申请人： Jose Cibelli ,  Michael D. West ,  Robert Lanza

    发明人： Jose Cibelli ,  Michael D. West ,  Robert Lanza

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N15/00

    CPC分类号： C12N5/16 ,  A61K35/12 ,  C12N5/0606 ,  C12N2510/00

    摘要： An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell.

    摘要翻译： 一种改进的产生分化祖细胞的方法，包括从囊胚获得内细胞质量细胞并诱导内细胞大量细胞的分化以产生分化祖细胞。 可以转染分化的祖细胞，使得存在所需基因的添加，缺失或改变。 分化的祖细胞可用于细胞治疗和作为用于产生移植组织和器官的细胞的I来源。 还提供了产生谱系缺陷型人胚胎干细胞的方法。

    LASER ISOLATION OF VIABLE CELLS
    7.
    发明申请
    LASER ISOLATION OF VIABLE CELLS 审中-公开
    激光分离可变电池

    公开(公告)号：US20120258451A1

    公开(公告)日：2012-10-11

    申请号：US13441617

    申请日：2012-04-06

    申请人： Irina Vitaly KLIMANSKAYA

    发明人： Irina Vitaly KLIMANSKAYA

    IPC分类号： C12N13/00 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53

    CPC分类号： C12N5/0621 ,  C12N2509/00 ,  G01N2001/2886

    摘要： Methods for laser microdissection isolation of viable cells are provided. Cells of a desired type may be isolated from a diverse population, optionally with detection and exclusion of undesired cells. Desired cells may be isolated from a population that arose from differentiation of pluripotent cells, preferably embryonic stem cells or induced pluripotent stem cells, and undifferentiated stem cells may be detected and excluded from selection including the isolation of RPE cells sleeted based on morphology (e.g., characteristic mottled appearance) from a population of ES cells. The cells isolated by these methods, including RPE cells, may be essentially free of undifferentiated cells and thus suitable for use in cell-based therapies.

    摘要翻译： 提供了活细胞激光显微切割分离方法。 所需类型的细胞可以从不同群体分离​​，任选地检测和排除不需要的细胞。 所需的细胞可以从多能细胞分化产生的群体分离，优选胚胎干细胞或诱导的多能干细胞，并且可以检测未分化的干细胞，并将其排除在选择之外，包括基于形态学分离的RPE细胞（例如， 特征斑驳的外观）从ES细胞群体。 通过这些方法分离的细胞（包括RPE细胞）可以基本上不含未分化细胞，因此适用于基于细胞的疗法。