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公开(公告)号：US10301563B2
公开(公告)日：2019-05-28
申请号：US15179469
申请日：2016-06-10
发明人： Rowena Justo Torres-Ordonez , Melanie Kuberka , Peter Placzek , Leslie R. Wolf , James J. Baustian
摘要： Gasoline blends and methods for producing gasoline blends containing high concentrations of a butanol isomer and having good cold start and warm-up driveability characteristics are disclosed.
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公开(公告)号：US10174345B2
公开(公告)日：2019-01-08
申请号：US15335628
申请日：2016-10-27
发明人： Yougen Li , Jessica McElvain , Steven Cary Rothman
摘要： Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed.
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公开(公告)号：US09944954B2
公开(公告)日：2018-04-17
申请号：US15228770
申请日：2016-08-04
发明人： Ritu Bhalla , Gopal K. Chotani , Michael Dauner , Mark J. Nelson , Daniel P. O'Keefe , Caroline M. Peres , Jahnavi Chandra Prasad , Jean-Francois Tomb
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N15/52 , C12Y101/01008 , C12Y101/01094 , C12Y101/05003 , C12Y102/01012 , Y02E50/10 , Y02P20/52
摘要： Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.
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公开(公告)号：US09790521B2
公开(公告)日：2017-10-17
申请号：US13428585
申请日：2012-03-23
申请人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
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公开(公告)号：US09689004B2
公开(公告)日：2017-06-27
申请号：US13833369
申请日：2013-03-15
发明人： Lori Ann Maggio-Hall
CPC分类号： C12P7/16 , C12N1/20 , C12Y101/01008 , C12Y101/01086 , C12Y102/01005 , C12Y401/01001 , C12Y402/01009 , Y02E50/10
摘要： The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.
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公开(公告)号：US09650624B2
公开(公告)日：2017-05-16
申请号：US14142398
申请日：2013-12-27
CPC分类号： C12P7/16 , C07K2319/00 , C12N9/88 , C12N15/00 , C12P7/40 , C12Y402/01009 , Y02E50/10
摘要： Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
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公开(公告)号：US09611482B2
公开(公告)日：2017-04-04
申请号：US13837893
申请日：2013-03-15
发明人： Dennis Flint , Brian James Paul , Rick W. Ye
IPC分类号： C12N15/81 , C12N9/88 , C07K14/395 , C12P7/16 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , G01N33/573
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12N15/81 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , C12Y402/01009 , G01N33/573 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
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公开(公告)号：US09517985B2
公开(公告)日：2016-12-13
申请号：US14772705
申请日：2014-03-14
发明人： Thomas Basham , Leslie William Bolton , Ian David Dobson , Sarah Richardson Hanson , Aidan Hurley , Karen Kustedjo , Andrew Richard Lucy , Liang Song
摘要： The invention relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water immiscible organic extractant during the fermentation. The invention also relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible extractant composition. The invention further relates to compositions comprising a solution of butanol in a water immiscible organic extractant composition.
摘要翻译： 本发明涉及通过微生物发酵生产丁醇的方法，其中通过在发酵期间通过萃取到与水不混溶的有机萃取剂中除去丁醇产物。 本发明还涉及通过微生物发酵生产丁醇的方法，其中在发酵期间通过萃取到与水不混溶的萃取剂组合物中除去丁醇产物。 本发明还涉及包含丁醇在水不混溶的有机萃取剂组合物中的溶液的组合物。
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9.发明授权公开(公告)号：US09404117B2
公开(公告)日：2016-08-02
申请号：US14848792
申请日：2015-09-09
CPC分类号： C12P7/16 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/81 , C12Y203/03013 , C12Y206/01042 , C12Y401/01001 , C12Y403/01019 , Y02E50/10 , Y02P20/52
摘要： Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.
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公开(公告)号：US09388392B2
公开(公告)日：2016-07-12
申请号：US14853108
申请日：2015-09-14
发明人： Sridhar Govindarajan , Yougen Li , Der-Ing Liao , Daniel P. O'Keefe , Jeremy Stephen Minshull , Steven Cary Rothman , Alexander Vincent Tobias
CPC分类号： C12N9/0006 , C12P7/16 , C12P7/42 , C12P7/44 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y101/01086 , Y02E50/10 , Y02P20/52
摘要： Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
摘要翻译： 本文提供具有酮酸酸还原异构酶活性的多肽以及包含这些多肽的微生物宿主细胞。 本文提供的多肽可用于生物合成途径，包括但不限于异丁醇生物合成途径。
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