公开(公告)号：US20100062471A1

公开(公告)日：2010-03-11

申请号：US12088228

申请日：2006-09-29

申请人： Aaron B. Kantor ,  Susan Goelz ,  Jun Deng ,  Hua Lin ,  Christopher Becker

发明人： Aaron B. Kantor ,  Susan Goelz ,  Jun Deng ,  Hua Lin ,  Christopher Becker

IPC分类号： C12Q1/02

CPC分类号： G01N33/5091 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/564 ,  G01N2500/00 ,  G01N2500/10 ,  G01N2800/285 ,  G01N2800/52

摘要： Biomarkers useful for identifying treatments for and monitoring treatment of patients with multiple sclerosis (MS) are provided, as well as methods for their identification, methods of diagnosing MS, relapse of MS patients and disease progression in MS patients.

摘要翻译： 提供了用于识别多发性硬化症（MS）患者治疗和监测治疗的生物标志物，以及其鉴定方法，MS诊断方法，MS患者复发和MS患者疾病进展。

公开(公告)号：US07387880B2

公开(公告)日：2008-06-17

申请号：US11075024

申请日：2005-03-07

申请人： Timothy L. Wyant ,  Paul A. Raju ,  Aaron B. Kantor

发明人： Timothy L. Wyant ,  Paul A. Raju ,  Aaron B. Kantor

IPC分类号： G01N33/53 ,  G01N1/00

CPC分类号： G01N33/86 ,  G01N15/1468 ,  G01N15/1484 ,  G01N2015/0084 ,  G01N2015/1465 ,  G01N2015/1472 ,  G01N2015/1486 ,  Y10T436/108331 ,  Y10T436/24 ,  Y10T436/2525

摘要： The amount of platelet surface proteins in a sample may be measured by collecting a sample containing platelets into a collection tube containing a platelet stabilizing composition, labeling the platelet surface protein and detecting by cytometry.

摘要翻译： 样品中血小板表面蛋白的量可以通过将含有血小板的样品收集到含有血小板稳定组合物的收集管中，标记血小板表面蛋白并通过细胞计数进行检测来测量。

公开(公告)号：US07253404B2

公开(公告)日：2007-08-07

申请号：US11173937

申请日：2005-07-01

申请人： Curtis Hastings

发明人： Curtis Hastings

IPC分类号： H01J49/00

CPC分类号： H01J49/0036 ,  G01N30/72 ,  G01N30/8624 ,  G01N30/8641 ,  H01J49/0431

摘要： High-intensity, spiked noise is reduced in chromatography-mass spectrometry data by applying a nonlinear filter such as a moving median filter to the data. The filter is applied to individual mass chromatograms, plots of ion abundance versus retention time for each detected mass-to-charge ratio, and the filtered chromatograms are combined to form a filtered total ion current chromatogram. Standard linear filters are not effective for reducing noise in liquid chromatography-mass spectrometry (LC-MS) data because they assume a normal distribution of noise. LC-MS noise, however, is not normally distributed.

摘要翻译： 通过对数据应用诸如移动中值滤波器的非线性滤波器，在色谱 - 质谱数据中降低了高强度，加标噪声。 将过滤器应用于单个质量色谱图，每种检测质量 - 电荷比的离子丰度曲线与保留时间曲线，并将滤出的色谱图合并形成过滤的总离子电流色谱图。 标准线性滤波器对于降低液相色谱 - 质谱（LC-MS）数据中的噪声无效，因为它们假设噪声的正态分布。 然而，LC-MS噪声不是正态分布的。

公开(公告)号：US07248360B2

公开(公告)日：2007-07-24

申请号：US11090673

申请日：2005-03-25

申请人： Uwe Horchner ,  Aaron B. Kantor

发明人： Uwe Horchner ,  Aaron B. Kantor

IPC分类号： G01J3/30 ,  G01J3/28 ,  G01J3/44 ,  G01J3/40 ,  G01J1/58 ,  F21V9/16 ,  G01T1/10 ,  G21H3/02 ,  G21K5/00 ,  H01J65/06 ,  H01J65/08

CPC分类号： G01J3/2803 ,  G01J3/44 ,  G01N15/1434 ,  G01N21/6428 ,  G01N21/658 ,  G01N2015/008 ,  G01N2015/144 ,  G01N2021/6482

摘要： A system for laser scanning provides spectral flexibility needed for the spectroscopic monitoring of highly multiplexed samples, such as cellular and particle assays in whole blood or other suspensions. In accordance with embodiments of the present invention, the system comprises a scanner to direct an excitation laser through a sample, an objective to collect light emitted by the sample in response to the excitation laser, a spectrograph to disperse the emitted light over a plurality of wavelengths as a spectrum, and a charge coupled device for detecting the spectrum. The system can be used with samples having a variety of reporter tags, including one or more SERS tags, fluorescent organic and protein tags, and quantum dot tags. A laser scanning apparatus and method of using the same is also provided.

摘要翻译： 用于激光扫描的系统提供了高分辨率样品的光谱监测所需的光谱灵活性，例如全血或其它悬浮液中的细胞和颗粒测定。 根据本发明的实施例，该系统包括扫描器，用于将激发激光引导通过样本，用于响应于激发激光收集由样本发射的光的目标，将发射的光分散在多个 波长作为光谱，以及用于检测光谱的电荷耦合器件。 该系统可以与具有各种报告标签的样品一起使用，包括一个或多个SERS标签，荧光有机和蛋白质标签以及量子点标签。 还提供了一种激光扫描装置及其使用方法。

公开(公告)号：US07197401B2

公开(公告)日：2007-03-27

申请号：US11075370

申请日：2005-03-07

申请人： Curtis A. Hastings

发明人： Curtis A. Hastings

IPC分类号： G06F19/00

CPC分类号： G01N30/8624 ,  G01N30/72 ,  G01N30/8631 ,  G01N30/8634 ,  H01J49/0036 ,  Y10T436/24

摘要： An automatic peak selection method for multidimensional data that selects peaks from very noisy data such as two-dimensional liquid chromatography-mass spectrometry (LC-MS) data is described. Such data are characterized by non-normally distributed noise that varies in different dimensions. The method computes local noise thresholds for each one-dimensional component of the data. Each point has a local noise threshold applied to it for each dimension of the data set, and a point is selected as a candidate peak only if its value exceeds all of the applied local noise thresholds. Contiguous candidate peaks are clustered into actual peaks. The method is preferably implemented as part of a high-throughput platform for analyzing complex biological mixtures.

摘要翻译： 描述了从诸如二维液相色谱 - 质谱（LC-MS）数据的非常嘈杂的数据中选出峰的多维数据的自动峰选择方法。 这种数据的特征在于不均匀分布的噪声在不同的维度上变化。 该方法计算数据的每个一维分量的局部噪声阈值。 每个点对于数据集的每个维度都具有应用于其的局部噪声阈值，并且仅当其值超过所有施加的局部噪声阈值时，才将点选择为候选峰。 相邻的候选峰聚类成实际峰。 优选地，该方法被实施为用于分析复杂生物混合物的高通量平台的一部分。

公开(公告)号：US20060259246A1

公开(公告)日：2006-11-16

申请号：US11327169

申请日：2006-01-05

申请人： Nam Huyn

发明人： Nam Huyn

IPC分类号： G06F19/00

CPC分类号： G16B40/00

摘要： A biological marker identification method identifies biological markers within broad sets of biological data containing many more measurements than observations. For example, the data can contain thousands of measurements on each blood sample obtained from fewer than 100 subjects, each of which falls into one of a set of clinical classes or is associated with a value of a continuous clinical response variable. At least one biomarker, containing a small subset of measurements, is found that is capable of predicting a clinical endpoint. The biomarker can be used for, e.g., diagnosing disease or assessing response to a drug. First, the set of measurements is reduced to a smaller set of candidate measurements by eliminating measurements that either cannot distinguish among classes or are redundant. Biomarker subsets are then selected from the remaining set of measurements, either by an exhaustive search or a heuristic method that finds good but not necessary globally optimal biomarkers.

公开(公告)号：US20060008856A1

公开(公告)日：2006-01-12

申请号：US11220746

申请日：2005-09-06

申请人： Rajendra Singh ,  Haihong Zhou

发明人： Rajendra Singh ,  Haihong Zhou

IPC分类号： G01N33/53 ,  C07K14/47

CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07D495/04 ,  G01N33/6851

摘要： The present invention relates generally to novel protein modification reagents for fractionation and quantitative (differential) profiling of proteins in a complex mixture. The reagents react with amino acids or other protein components or structures and function as mass tags. The present invention provides methods of making the protein modification reagents and methods of using the protein modification reagents for quantitative analysis of proteins.

公开(公告)号：US20050266467A1

公开(公告)日：2005-12-01

申请号：US11134046

申请日：2005-05-19

申请人： Sushmita Roy

发明人： Sushmita Roy

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/564 ,  G01N33/567 ,  G01N33/68

CPC分类号： G01N33/6896 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/564 ,  G01N33/6848 ,  G01N2500/04 ,  G01N2800/285

摘要： Disclosed are biomarkers, the expression of which is differentially regulated in subjects with multiple sclerosis (MS) as compared to subjects that do not have MS. Also described are methods of identification of such biomarkers, and methods of using such biomarkers as targets for the development and identification of therapeutic compounds and strategies for the treatment of MS, as well as methods and kits for the diagnosis of MS.

摘要翻译： 公开了与不具有MS的受试者相比，其表达在具有多发性硬化（MS）的受试者中差异调节的生物标志物。 还描述了鉴定这样的生物标志物的方法，以及使用这样的生物标志物作为用于开发和鉴定治疗化合物的靶标的方法以及用于治疗MS的策略，以及用于诊断MS的方法和试剂盒。

公开(公告)号：US06937330B2

公开(公告)日：2005-08-30

申请号：US10421638

申请日：2003-04-22

申请人： Louis J. Dietz ,  Christopher E. Todd

发明人： Louis J. Dietz ,  Christopher E. Todd

IPC分类号： B01L3/00 ,  B01L9/00 ,  G01N21/05 ,  G01N21/11 ,  G01N21/25 ,  G01N21/64 ,  G01N1/10 ,  G01N33/00

CPC分类号： B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0406 ,  G01N21/03 ,  G01N21/05 ,  G01N21/11 ,  G01N21/253 ,  G01N21/278 ,  G01N21/645 ,  G01N2021/0346

摘要： A disposable cuvette cartridge for optical measurements is formed from three flexible thin layers. The middle layer is an adhesive sheet with cut out regions, typically between 30 and 50, serving as optical chambers bounded by the two outer layers. Inlet and vent holes in one of the outer sheets provide access to the optical chambers. At least one of the outer sheets is made of a material having low fluorescence, such as a cyclo-olefin copolymer. With its low background fluorescence, the cuvette cartridge is useful for high-throughput fluorescence measurements of biological samples.

摘要翻译： 用于光学测量的一次性比色皿盒由三个柔性薄层形成。 中间层是具有切口区域的粘合片，通常在30和50之间，用作由两个外层限定的光学室。 外片之一中的入口孔和通气孔提供对光学室的通路。 外片中的至少一个由具有低荧光的材料制成，例如环烯烃共聚物。 采用低背景荧光，比色杯筒可用于生物样品的高通量荧光测量。

公开(公告)号：US20100015717A1

公开(公告)日：2010-01-21

申请号：US12391119

申请日：2009-02-23

申请人： Rajendra Singh ,  Haihong Zhou

发明人： Rajendra Singh ,  Haihong Zhou

IPC分类号： G01N33/68 ,  C07K2/00 ,  C07D235/02 ,  C07D401/00 ,  C07D409/00

CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07D495/04 ,  G01N33/6851

摘要： The present invention relates generally to novel protein modification reagents for fractionation and quantitative (differential) profiling of proteins in a complex mixture. The reagents react with amino acids or other protein components or structures and function as mass tags. The present invention provides methods of making the protein modification reagents and methods of using the protein modification reagents for quantitative analysis of proteins.

摘要翻译： 本发明一般涉及用于复合混合物中蛋白质的分级和定量（差异）分布的新型蛋白质修饰试剂。 试剂与氨基酸或其他蛋白质组分或结构物反应，起到质量标签的作用。 本发明提供了制备蛋白质修饰试剂的方法和使用蛋白质修饰试剂进行蛋白质定量分析的方法。