公开(公告)号：US20150017738A1

公开(公告)日：2015-01-15

申请号：US14380147

申请日：2013-02-22

申请人： IMMUNDIAGNOSTIK AG

发明人： Franz Paul Armbruster ,  Berthold Hocher ,  Hans Juergen Groen ,  Heinz Juergen Roth

IPC分类号： G01N33/78 ,  C07K16/26

CPC分类号： G01N33/78 ,  C07K16/26 ,  C07K2317/30 ,  C07K2317/34 ,  G01N33/6848 ,  G01N33/6854 ,  G01N33/6893 ,  G01N2333/635 ,  G01N2500/00 ,  G01N2800/046 ,  G01N2800/347 ,  G01N2800/7009

摘要： Method for obtaining an antibody or antibody fragment to a conformational epitope specific for oxidized, inactive human parathyroid hormone and fragments thereof; a method for removal of oxidized, inactive human parathyroid hormone from a sample of body fluid; methods of determining the concentration of active parathyroid hormone in a sample, and an in vitro method of diagnosis of renal failure or secondary hyperthyroidism in patients on dialysis. The antibody is obtained by immunizing an animal with an immunogen containing parathyroid hormone or fragment thereof oxidized at methionines at positions 8, 18 or both; and a recovering of antibodies; whereby the complementary determining region of the antibody or antibody fragment or single chain antibody specifically recognizes a conformational epitope (antigenic determinant) which is a tertiary structure and only present on oxidized parathyroid hormone and fragments thereof only but not regular bioactive human parathyroid hormone, while the binding surface to the complementary binding region does not include any one of the oxidized methionines of human parathyroid hormone.

摘要翻译： 获得针对氧化的，无活性的人甲状旁腺激素及其片段特异的构象表位的抗体或抗体片段的方法; 从体液样品中去除氧化的，无活性的人甲状旁腺激素的方法; 确定样品中活性甲状旁腺激素浓度的方法，以及透析患者肾衰竭或继发性甲状腺功能亢进的体外诊断方法。 抗体是通过用含有甲状旁腺激素或其片段的蛋白质在8,18或两者氧化的免疫原免疫动物获得的; 和抗体的回收; 由此抗体或抗体片段或单链抗体的互补决定区域特异性识别作为三级结构的构象表位（抗原决定簇），仅存在于氧化的甲状旁腺激素及其片段但不存在于规则的生物活性人甲状旁腺激素上，而 与互补结合区的结合表面不包括人甲状旁腺激素的氧化甲硫氨酸中的任一种。

公开(公告)号：US08012694B2

公开(公告)日：2011-09-06

申请号：US11521007

申请日：2006-09-14

申请人： Richard J. Zahradnik ,  Jeffrey R. Lavigne

发明人： Richard J. Zahradnik ,  Jeffrey R. Lavigne

IPC分类号： A61K38/29 ,  C07K16/06 ,  C07K16/26 ,  G01N33/53 ,  G01N33/566 ,  G01N33/577

CPC分类号： G01N33/74 ,  C07K16/26 ,  C07K2317/34 ,  G01N2333/635

摘要： Antibodies for a variant of intact parathyroid hormone (PTH) or its fragments which have at position 17 the amino acid serine which is phosphorylated [p(17) PTH] and methods of producing the same. The invention further includes various immunoassay methods which use the described antibody alone or in combination with other antibodies to determine the circulating levels of p(17) intact PTH (1-84) or fragments thereof in biological fluids. Such antibodies and immunoassays can be used in conjunction with other antibodies to detect all biologically active forms of hPTH present in biological fluids.

摘要翻译： 在17位具有磷酸化的氨基酸丝氨酸[p（17）PTH）的完整甲状旁腺激素（PTH）或其片段的变体的抗体及其制备方法。 本发明还包括各种免疫测定方法，其单独使用所述抗体或与其它抗体组合，以确定生物流体中p（17）完整PTH（1-84）或其片段的循环水平。 这样的抗体和免疫测定可与其它抗体结合使用以检测存在于生物流体中的所有生物活性形式的hPTH。

公开(公告)号：US20070141717A1

公开(公告)日：2007-06-21

申请号：US11311567

申请日：2005-12-19

申请人： Charles Carpenter ,  Giosi Farace ,  Paul MacHenry ,  Brian Foster ,  Mirolee Zieba ,  Michael D'Angelo

发明人： Charles Carpenter ,  Giosi Farace ,  Paul MacHenry ,  Brian Foster ,  Mirolee Zieba ,  Michael D'Angelo

IPC分类号： G01N21/76

CPC分类号： G01N33/58 ,  G01N2333/635

摘要： A method and a kit for determining the presence or amount of analytes in samples over a broad potential concentration range for the analyte. The method and kit incorporate chemiluminescent and fluorescent labels conjugated to a specific binding partner for the analyte for sandwich assays, or to the analyte or an analog of the analyte for competitive assays. The conjugates are mixed with the sample and the labels are detected simultaneously or sequentially.

摘要翻译： 用于在分析物的广泛潜在浓度范围内确定样品中分析物的存在或量的方法和试剂盒。 该方法和试剂盒包含化学发光和荧光标记，其结合到用于夹心测定的分析物的特异性结合配偶体，或用于竞争性测定的分析物或分析物的类似物。 将缀合物与样品混合，同时或依次检测标记。

公开(公告)号：US20030119744A1

公开(公告)日：2003-06-26

申请号：US10291360

申请日：2002-11-08

发明人： Shafaat Ahmed Rabbani ,  Nicholas Shukeir ,  Chandra J. Panchal ,  Christopher Newman

IPC分类号： A61K038/17

CPC分类号： A61K38/1709 ,  C07K14/47 ,  G01N33/57434 ,  G01N33/84 ,  G01N2333/635 ,  G01N2500/00 ,  G01N2800/04

摘要： The present invention discloses the use of PSP-94 for treating a patient suffering from hypercalcemia of malignancy. Prostate secretory protein of 94 amino acids (PSP-94) is known to serve as prognostic marker in hormone-dependent prostate. In the current study the effect of PSP-94 on prostate cancer growth, metastases to the skeleton and hypercalcemia of malignancy was examined. A marked dose-dependent decrease in primary tumor volume was seen in experimental animals receiving PSP-94. Furthermore, while control animals routinely developed hypercalcemia due to PTHrP production, treatment with PSP-94 led to a near normalization of plasma calcium and a marked reduction in PTHrP production as determined by radioimmunoassay and immunohistochemistry. Results obtained demonstrate the ability of PSP-94 to be an effective treatment modality for prostate cancer. Furthermore, decrease in plasma PTHrP and calcium levels can serve as useful biochemical markers for monitoring the efficacy of this novel anti-tumor agent.

摘要翻译： 本发明公开了PSP-94用于治疗恶性高钙血症患者的用途。 已知94个氨基酸（PSP-94）的前列腺分泌蛋白用作激素依赖性前列腺中的预后标志物。 在目前的研究中，检查了PSP-94对前列腺癌生长，骨骼转移和恶性高钙血症的影响。 在接受PSP-94的实验动物中观察到原发肿瘤体积明显的剂量依赖性降低。 此外，虽然对照动物由于PTHrP产生而常规地发展为高钙血症，但通过放射免疫测定和免疫组织化学测定，用PSP-94处理导致血浆钙几乎正常化和PTHrP产生显着降低。 获得的结果表明PSP-94是前列腺癌的有效治疗方法的能力。 此外，血浆PTHrP和钙水平的降低可以用作监测这种新型抗肿瘤剂的功效的有用的生化标志物。

公开(公告)号：US20150038595A1

公开(公告)日：2015-02-05

申请号：US14131566

申请日：2012-07-05

申请人： ASTUTE MEDICAL, INC.

发明人： Joseph Anderberg ,  Jeff Gray ,  Paul McPherson ,  Kevin Nakamura ,  James Patrick Kampf

IPC分类号： G01N33/68 ,  G01N33/74

CPC分类号： G01N33/6893 ,  G01N33/74 ,  G01N33/743 ,  G01N2333/47 ,  G01N2333/4727 ,  G01N2333/4756 ,  G01N2333/575 ,  G01N2333/635 ,  G01N2333/765 ,  G01N2333/96433 ,  G01N2333/96463 ,  G01N2800/347 ,  G01N2800/50 ,  G01N2800/54 ,  G01N2800/56 ,  G01N2800/60

摘要： The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Serum albumin, Protein S100-B, Glial cell line-derived neurotrophic factor, Resistin, Serum amyloid A protein, Hydrocortisone, Parathyroid hormone, and Tissue Plasminogen Activator as diagnostic and prognostic biomarkers in renal injuries.

摘要翻译： 本发明涉及用于在患有或疑似患有肾损伤的受试者中监测，诊断，预后和确定治疗方案的方法和组合物。 特别地，本发明涉及使用一种或多种测定法，其被配置为检测选自以下的肾损伤标记物：血清白蛋白，蛋白质S100-B，胶质细胞系衍生的神经营养因子，抗性蛋白，血清淀粉样蛋白A蛋白，氢化可的松 ，甲状旁腺激素和组织纤溶酶原激活剂作为肾损伤的诊断和预后生物标志物。

公开(公告)号：US08293479B2

公开(公告)日：2012-10-23

申请号：US10513474

申请日：2003-05-01

申请人： John S. Torday ,  Virender K. Rehan ,  Richard Mink

发明人： John S. Torday ,  Virender K. Rehan ,  Richard Mink

IPC分类号： G01N33/53 ,  G01N33/00 ,  G01N33/567 ,  A01N43/40

CPC分类号： G01N33/78 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2333/635 ,  G01N2800/12 ,  G01N2800/52

摘要： This invention pertains to the discovery that Parathyroid Hormone-related Protein (PTHrP) can be detect and/or stage, and/or treat chronic lung diseases. In particular, it was discovered that PTHrP levels in broncho-alveolar lavage are indicative of lung “health” and “disease”, and can be used to predict lung disease in patients at risk of chronic lung disease and/or to evaluate the efficacy of a ventilation regime.

摘要翻译： 本发明涉及甲状旁腺激素相关蛋白（PTHrP）可以被检测和/或分期和/或治疗慢性肺部疾病的发现。 特别是，发现支气管肺泡灌洗液中的PTHrP水平是肺健康和疾病的指标，可用于预测患有慢性肺部疾病风险的患者的肺部疾病和/或评估通气机制的功效。

公开(公告)号：US20070098726A1

公开(公告)日：2007-05-03

申请号：US11516912

申请日：2006-09-06

申请人： Thomas Cantor ,  Zan Yang

发明人： Thomas Cantor ,  Zan Yang

IPC分类号： A61K39/395 ,  C12P21/04 ,  C07K16/26

CPC分类号： C07K16/26 ,  G01N2333/635

摘要： The present invention relates to methods of generating anti-PTH antibodies and antibodies generated by the methods of the invention. The anti-PTH antibodies generated by the methods of the invention specifically bind to an epitope on a whole PTH (1-84) which includes one or more N-terminal amino acid residues of the whole PTH. The anti-PTH antibodies generated are useful for detecting whole PTH in a biological sample and for diagnosing parathyroid diseases or disorders.

摘要翻译： 本发明涉及通过本发明的方法产生的抗PTH抗体和抗体的方法。 通过本发明方法产生的抗PTH抗体特异性结合整个PTH（1-84）上的表位，其包括整个PTH的一个或多个N-末端氨基酸残基。 产生的抗PTH抗体可用于检测生物样品中的全部PTH和诊断甲状旁腺疾病或病症。

公开(公告)号：US20060035282A1

公开(公告)日：2006-02-16

申请号：US11144437

申请日：2005-06-03

申请人： Thomas Cantor

发明人： Thomas Cantor

IPC分类号： G01N33/53 ,  G06F19/00 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N33/6893 ,  G01N33/74 ,  G01N2333/635 ,  G01N2800/345

摘要： The present invention relates to identifying the risk of a subject developing renal stones utilizing an assessment of PTH agonist, antagonist and/or total PTH levels, optionally together with an assessment of serum and/or urine calcium levels and/or other analytes. The present description further relates to monitoring and guiding treatment for renal stones and kits useful therefore.

摘要翻译： 本发明涉及使用PTH激动剂，拮抗剂和/或总PTH水平的评估，可选地与血清和/或尿钙水平和/或其他分析物的评估来鉴定发展为肾结石的受试者的风险。 本描述进一步涉及用于肾结石的监测和指导治疗以及因此有用的试剂盒。

公开(公告)号：US20060024772A1

公开(公告)日：2006-02-02

申请号：US11146598

申请日：2005-06-06

申请人： James Hutchison

发明人： James Hutchison

IPC分类号： G01N33/53 ,  G01N33/537 ,  C07K16/26

CPC分类号： G01N33/78 ,  C07K16/26 ,  C07K2317/34 ,  G01N2333/635

摘要： Methods of preparing antibodies that recognize and bind three-dimensional epitopes of antigens are disclosed. The methods are particularly useful for preparing antibodies that bind the bioactive, three-dimensional amino terminus of parathyroid hormone. The antibodies so produced are used in diagnostic and therapeutic applications.

公开(公告)号：US20050003493A1

公开(公告)日：2005-01-06

申请号：US10921368

申请日：2004-08-18

申请人： James Hutchison

发明人： James Hutchison

IPC分类号： G01N33/53 ,  A61K39/395 ,  A61P3/14 ,  A61P5/20 ,  A61P43/00 ,  C07K16/26 ,  C12P21/08 ,  G01N21/76 ,  G01N30/88 ,  G01N33/543 ,  C12P21/04

CPC分类号： G01N33/78 ,  C07K16/26 ,  C07K2317/34 ,  G01N2333/635

摘要： Methods of preparing antibodies that recognize and bind three-dimensional epitopes of antigens are disclosed. The methods are particularly useful for preparing antibodies that bind the bioactive, three-dimensional amino terminus of parathyroid hormone. The antibodies so produced are used in diagnostic and therapeutic applications.

摘要翻译： 公开了制备识别和结合抗原三维表位的抗体的方法。 该方法对于制备结合甲状旁腺激素的生物活性的三维氨基末端的抗体特别有用。 如此制备的抗体用于诊断和治疗应用。