公开(公告)号：US20240352500A1

公开(公告)日：2024-10-24

申请号：US18684500

申请日：2022-08-18

申请人： Max-Delbrück-Centrum für Molekulare Medizin ,  Humboldt-Universität zu Berlin

发明人： Markus Landthaler ,  Ulrike Zinnall ,  Igor Minia

IPC分类号： C12P21/02 ,  C07K14/505 ,  C07K14/565 ,  C07K14/59 ,  C07K14/62 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC分类号： C12P21/02 ,  C07K14/505 ,  C07K14/565 ,  C07K14/59 ,  C07K14/62 ,  C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C07K2319/30 ,  C12N2310/20

摘要： The invention is related to a method for producing a secreted recombinant protein of interest in a cell, wherein the method comprises artificially co-expressing a protein different from the secreted recombinant protein of interest.

公开(公告)号：US20240350595A1

公开(公告)日：2024-10-24

申请号：US18392854

申请日：2023-12-21

申请人： The Trustees of the University of Pennsylvania

发明人： Daniel Rader ,  Devin Christopher ,  Anna P. Tretiakova

IPC分类号： A61K38/45 ,  A61K38/00 ,  A61K48/00 ,  A61P1/16 ,  A61P9/00 ,  C12N9/10 ,  C12N15/86 ,  C12P21/02

CPC分类号： A61K38/45 ,  A61K48/0058 ,  A61P1/16 ,  A61P9/00 ,  C12N9/1029 ,  C12N15/86 ,  C12P21/02 ,  A61K38/00 ,  C12N2750/14143 ,  C12Y203/01043

摘要： A synthetic or recombinant human lecithin cholesterol acyltransferase (LCAT) variant is provided which comprises an LCAT enzyme having a substitution at position 114 based on the residue numbering of wild-type (WT) human LCAT [SEQ ID NO:1], wherein said variant is characterized by one or more of: (i) an esterification rate higher than the esterification rate of WT human LCAT; and/or (ii) an association with higher density lipoprotein levels as compared to subjects having WT LCAT. Also provided are vectors encoding the variant, compositions containing same, and methods of using the variant proteins and vectors for treatment of a variety of disorders associated with defective wt LCAT.

公开(公告)号：US12122810B2

公开(公告)日：2024-10-22

申请号：US17751277

申请日：2022-05-23

申请人： BOLT THREADS, INC.

发明人： Joshua Kittleson ,  Thomas Stevens ,  Rena Hill ,  Carlos Gustavo Pesce

IPC分类号： C07K14/435 ,  C07K14/395 ,  C12N1/19 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02

CPC分类号： C07K14/43586 ,  C07K14/395 ,  C12N15/625 ,  C12N15/815 ,  C12P21/02 ,  C07K2319/02 ,  C07K2319/036

摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.

公开(公告)号：US20240336947A1

公开(公告)日：2024-10-10

申请号：US18626636

申请日：2024-04-04

申请人： University of Kentucky Research Foundation

发明人： Houfu Guo

IPC分类号： C12P21/02 ,  C12N9/10

CPC分类号： C12P21/02 ,  C12N9/1051 ,  C12Y204/01066

摘要： Humans and Acanthamoeba Polyphaga Mimivirus share numerous homologous genes, including collagens and collagen-modifying enzymes. To explore the homology, a genome-wide comparison was performed between human and mimivirus using DELTA-BLAST (Domain Enhanced Lookup Time Accelerated BLAST) and identified 190 new mimiviral proteins that share homology with 1236 human proteins. To gain functional insights into mimiviral proteins, the human protein homologs were organized into Gene Ontology (GO) and REACTOME pathways to build a functional network. Collagen and collagen-modifying enzymes form the largest subnetwork with most nodes. Further analysis of this subnetwork identified a putative collagen glycosyltransferase R699. Protein expression test suggested that R699 is highly expressed in E coli, unlike the human collagen-modifying enzymes. Enzymatic activity assays showed that R699 catalyzes the conversion of galactosyl-hydroxylysine to glucosyl-galactosyl-hydroxylysine on collagen using UDP-glucose as a sugar donor, suggesting R699 is a mimiviral collagen galactosylhydroxylysyl glucosyltransferase (GGT). Structural study of R699 produced the first crystal structure of a collagen GGT with uridine diphosphate glucose (UDP-Glc). Sugar moiety of the UDP-Glc resides in a previously unrecognized pocket. Mn2+ coordination and nucleoside-diphosphate binding site are conserved among GGT family members and critical for R699's collagen GGT activity. To facilitate further analysis of human and mimiviral homologous proteins, we presented an interactive and searchable genome-wide comparison app for quickly browsing of human and Acanthamoeba Polyphaga Mimivirus homologs, which is available at RRID Resource ID: SCR_022140 or guolab.shinyapps.io/app-mimivirus-publication/.

公开(公告)号：US20240317835A1

公开(公告)日：2024-09-26

申请号：US18733576

申请日：2024-06-04

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC分类号： C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC分类号： C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

摘要： The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12091699B2

公开(公告)日：2024-09-17

申请号：US17586234

申请日：2022-01-27

申请人： Korea Advanced Institute of Science and Technology

发明人： Jin Woo Kim ,  Jun Woo Park

IPC分类号： C12P21/02

CPC分类号： C12P21/02

摘要： A composition for enhancing the production of a homeoprotein includes an inhibitor of lysosomal function. A method for enhancing the production of a homeoprotein, includes treating cells with an inhibitor of lysosomal function, a method for producing an animal cell line continuously expressing a homeoprotein and a method for mass-producing a homeoprotein using the animal cell line.

公开(公告)号：US20240295570A1

公开(公告)日：2024-09-05

申请号：US18594431

申请日：2024-03-04

申请人： UNIVERSITY OF SOUTH FLORIDA

发明人： Gopal Thinakaran

IPC分类号： G01N33/68 ,  C12P21/02

CPC分类号： G01N33/6896 ,  C12P21/02

摘要： Provided herein are isolated BIN1 SH3 domain proteins and methods for their use to identify candidate Alzheimer's disease therapeutics.

公开(公告)号：US20240294964A1

公开(公告)日：2024-09-05

申请号：US18439838

申请日：2024-02-13

申请人： IKARIA INC.

发明人： Stephen DiMagno

IPC分类号： C12P21/02 ,  C07B59/00 ,  C07C233/51 ,  C07C327/42 ,  C07C327/44 ,  C07K14/54 ,  C07K14/545 ,  C12N9/00

CPC分类号： C12P21/02 ,  C07B59/001 ,  C07B59/008 ,  C07C233/51 ,  C07C327/42 ,  C07C327/44 ,  C07K14/54 ,  C07K14/545 ,  C12N9/93 ,  C12Y601/01026 ,  C07B2200/05

摘要： Provided herein are compositions and methods for generating polypeptides using non-natural amino acids (nnAAs) and genetic machinery, wherein the modified polypeptides, such as therapeutic polypeptides, bind to albumin, such as serum albumin. Methods of substituting a non-natural amino acid in a first polypeptide to obtain a modified polypeptide, the nnAA in some instances comprising an albumin targeting group, are disclosed, as are methods for making populations of such modified polypeptides. A therapeutic polypeptide, interleukin-1 receptor antagonist (IL-1RA) is exemplified using the disclosed methods.

公开(公告)号：US20240287569A1

公开(公告)日：2024-08-29

申请号：US18405235

申请日：2024-01-05

申请人： Zhejiang University ,  Ningbo Innovation Center, Zhejiang University ,  Shanghai Institute for Advanced Study of ZJU

发明人： Wei CHEN ,  Lianghua XIE ,  Yunhe MENG ,  Kaihao YOU ,  Jingwen ZHOU ,  Xin GUAN

IPC分类号： C12P21/02 ,  C12N9/16 ,  C12N9/88 ,  C12N15/70

CPC分类号： C12P21/02 ,  C12N9/16 ,  C12N9/88 ,  C12N15/70 ,  C12Y301/03001 ,  C12Y402/0201 ,  C12N2800/101

摘要： The present disclosure provides myoglobin (MB) and an expression vector and an expression engineering bacterium thereof, and use thereof, and relates to the technical field of genetic engineering. In the present disclosure, recombinant Escherichia coli strains with signal peptides Pel B and Omp A inserted under same conditions have an expression level of Sus scrofa myoglobin (SsMB) increased by 2.06 and 1.17 times, respectively, compared with an original expression strain of the SsMB. The signal peptides that can increase the expression level of the SsMB provide a new idea for research and application of improving the expression level of the SsMB.

公开(公告)号：US12071458B2

公开(公告)日：2024-08-27

申请号：US17530456

申请日：2021-11-18

申请人： IMMATICS BIOTECHNOLOGIES GMBH

发明人： Andrea Mahr ,  Toni Weinschenk ,  Helen Hoerzer ,  Oliver Schoor ,  Jens Fritsche ,  Harpreet Singh

IPC分类号： A61K39/00 ,  A61K35/17 ,  C07K7/06 ,  C07K7/08 ,  C07K14/47 ,  C07K14/705 ,  C07K14/74 ,  C07K14/82 ,  C07K16/18 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C12N5/0783 ,  C12N15/115 ,  C12P21/02 ,  C12Q1/6881 ,  C12Q1/6886 ,  G01N33/569 ,  G01N33/574 ,  A61K35/12 ,  A61K38/00

CPC分类号： C07K14/4748 ,  A61K35/17 ,  A61K39/0011 ,  C07K7/06 ,  C07K7/08 ,  C07K14/4705 ,  C07K14/4738 ,  C07K14/705 ,  C07K14/70539 ,  C07K14/82 ,  C07K16/18 ,  C07K16/28 ,  C07K16/2833 ,  C07K16/30 ,  C07K16/3015 ,  C07K16/3023 ,  C07K16/303 ,  C07K16/3038 ,  C07K16/3046 ,  C07K16/3053 ,  C07K16/3069 ,  C07K16/32 ,  C12N5/0636 ,  C12N15/115 ,  C12P21/02 ,  C12Q1/6881 ,  C12Q1/6886 ,  G01N33/56977 ,  G01N33/57449 ,  G01N33/57492 ,  A61K2035/124 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/5158 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2310/16 ,  C12N2320/30 ,  C12N2501/50 ,  C12N2501/998 ,  C12Q2600/158 ,  G01N2333/70539

摘要： The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.