-
公开(公告)号：US12083512B2
公开(公告)日：2024-09-10
申请号：US16069824
申请日：2017-01-13
发明人： Hongxing Hu , Samantha Seah , Christoph A. Merten
IPC分类号： B01L3/00 , C07K16/28 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C40B20/08
CPC分类号： B01L3/502715 , B01L3/502761 , C07K16/28 , C12Q1/6806 , C12Q1/6846 , C12Q1/686 , B01L2200/0647 , B01L2300/021 , C12Q2600/158 , C12Q2600/16 , C12Q2600/166 , C40B20/08 , C12Q1/6846 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
摘要： The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.
-
公开(公告)号：US20230324358A1
公开(公告)日：2023-10-12
申请号：US17979453
申请日：2022-11-02
发明人： Ken-ichi YAMADA , Saki SHINTO , Tomomi IDE , Keiichi YAMAMOTO
摘要： The present invention provides: an assay method that uses a compound represented by formula (I) as a fluorescent probe molecule and that is for detecting the lipid peroxidation suppression activity of a test compound; an assay kit that uses the assay method; a screening method that uses the assay method; and a pharmaceutical composition that is for the treatment, etc. of diseases (such as age-related macular degeneration) that are induced by lipid peroxidation reactions.
-
公开(公告)号：US11519893B2
公开(公告)日：2022-12-06
申请号：US16628446
申请日：2018-07-05
发明人： Ken-ichi Yamada , Saki Shinto , Tomomi Ide , Keiichi Yamamoto
IPC分类号： G01N33/00 , G01N33/15 , C01G49/14 , C07C57/00 , C07D413/12 , C12N5/071 , C40B20/08 , G01N33/52
摘要： The present invention provides: an assay method that uses a compound represented by formula (I) as a fluorescent probe molecule and that is for detecting the lipid peroxidation suppression activity of a test compound; an assay kit that uses the assay method; a screening method that uses the assay method; and a pharmaceutical composition that is for the treatment, etc. of diseases (such as age-related macular degeneration) that are induced by lipid peroxidation reactions.
-
公开(公告)号：US20180066262A1
公开(公告)日：2018-03-08
申请号：US15557010
申请日：2016-03-09
申请人： Caris Science, Inc.
发明人： Valeriy DOMENYUK , Adam STARK , Nianqing XIAO , Zhenyu ZHONG , Mark MIGLARESE , David SPETZLER
IPC分类号： C12N15/115 , C12Q1/68 , C40B20/08 , G01N33/50
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/3519 , C12N2320/10 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C40B20/08 , G01N33/5005
摘要： Methods and compositions are provided for oligonucleotide probes and oligonucleotide probe libraries that recognize targets of interest. The targets include circulating biomarkers such as microvesicles, including those derived from various diseases.
-
公开(公告)号：US09842198B2
公开(公告)日：2017-12-12
申请号：US14405415
申请日：2013-06-05
申请人： McMaster University
发明人： Nathan Magarvey , Aubrey Bailey Morgan Wyatt , Chad William Johnston , Ashraf Ibrahim , Bin Ma , Lian Yang
IPC分类号： G01N33/48 , G01N33/50 , G06F19/00 , G01N33/68 , C40B30/02 , G06F19/16 , G06F19/18 , C40B20/08 , G01N30/86 , G01N30/72
CPC分类号： G06F19/703 , C40B20/08 , C40B30/02 , G01N30/7233 , G01N30/86 , G01N33/6848 , G06F19/16 , G06F19/18
摘要： The present application is directed to methods and systems for identifying small molecule compounds in mixtures using a library comprising calculated structures and corresponding calculated mass spectral fragmentation patterns of known and/or hypothetical small molecule compounds that may be in the mixture and screening of a mass spectrum of the mixture using the library to identify matching fragmentation patterns. If a mass spectral fragmentation pattern present in the mass spectrum of the mixture matches a calculated fragmentation pattern of one of the known or hypothetical compounds this confirms the identity of a compound in the mixture as the known or hypothetical compound. The method represents a platform method that can be used for a multitude of purposes related to the screening and identification of compounds in mixtures. Therefore the methods and systems of the present application represent an approach that is uniquely capable of navigating chemical space and providing a understanding of desired families and pharmacophores.
-
6.发明授权公开(公告)号：US09817006B2
公开(公告)日：2017-11-14
申请号：US15164402
申请日：2016-05-25
申请人： Amy J. Reisinger
发明人： Amy J. Reisinger
CPC分类号： G01N33/94 , G01N30/7233 , G01N33/487 , H01J49/004
摘要： The present invention provides a rapid, sensitive method for forensic drug testing in a post-mortem subject using oral fluid collected from the post-mortem subject. The method comprises collecting a sample of oral fluid from a post-mortem subject, analyzing the oral fluid sample qualitatively to detect the presence of one or more non-naturally occurring drugs, analyzing the oral fluid sample quantitatively to determine concentration of the one or more non-naturally occurring drugs in the post-mortem subject, and identifying the one or more non-naturally occurring drugs in the post-mortem subject. The detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem subject. Further, the qualitative and quantitative results are obtained in as little as three hours.
-
公开(公告)号：US09719191B2
公开(公告)日：2017-08-01
申请号：US11998466
申请日：2007-11-29
CPC分类号： C40B20/08 , C12Q1/6811 , C40B30/04 , C40B40/08 , C40B40/12 , G01N33/5308 , G01N33/6845 , C12Q2565/531 , C12Q2525/205
摘要： Disclosed are methods for identifying a nucleic acid (e.g., RNA, DNA, etc.) motif which interacts with a ligand. The method includes providing a plurality of ligands immobilized on a support, wherein each particular ligand is immobilized at a discrete location on the support; contacting the plurality of immobilized ligands with a nucleic acid motif library under conditions effective for one or more members of the nucleic acid motif library to bind with the immobilized ligands; and identifying members of the nucleic acid motif library that are bound to a particular immobilized ligand. Also disclosed are methods for selecting, from a plurality of candidate ligands, one or more ligands that have increased likelihood of binding to a nucleic acid molecule comprising a particular nucleic acid motif, as well as methods for identifying a nucleic acid which interacts with a ligand.
-
公开(公告)号：US09404155B2
公开(公告)日：2016-08-02
申请号：US13860769
申请日：2013-04-11
发明人： Scott Bortner
CPC分类号： C12Q1/6874 , C12Q1/6869 , C40B20/08 , G06F19/22 , C12Q2565/301 , C12Q2537/149 , C12Q2535/101 , C12Q2565/627 , C12Q2533/107
摘要： Embodiments are provided that provide for parallel sequencing of nucleic acid segments. In some embodiments, a single sequence is sequenced by at least two different sequencing techniques and the results compared, allowing for deficiencies or strengths of one technique to be complemented by the second technique.
摘要翻译： 提供了提供核酸片段的并行测序的实施方案。 在一些实施方案中，通过至少两种不同的测序技术对单个序列进行测序，并且比较结果，允许通过第二种技术补充一种技术的缺陷或强度。
-
公开(公告)号：US08791053B2
公开(公告)日：2014-07-29
申请号：US10529397
申请日：2003-09-26
申请人： Soren Flygenring Christensen , Jens Hog Truelsen , Morten Meldal , Roice Michael , Ib Johannsen
发明人： Soren Flygenring Christensen , Jens Hog Truelsen , Morten Meldal , Roice Michael , Ib Johannsen
CPC分类号： B01J19/0046 , B01J2219/005 , B01J2219/0054 , B01J2219/00551 , B01J2219/00576 , B01J2219/00585 , B01J2219/00695 , B01J2219/00698 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/0074
摘要： The invention relates to a spatially encoded polymer matrix in the form of a bead or a granule for combinatorial solid phase synthesis, assaying, functional proteomics and diagnostic use. Compositions of such beads or granules are also provided. Each beaded polymer matrix of the composition comprises a plurality of spatially immobilized particles. The spatial immobilization of the particles confers on each beaded polymer matrix a “fingerprint” which enables identification of unique beads in a population of beads. The unique identification of individual beads makes it possible to perform combinatorial chemistry strategies while logging individual chemical transformation. Also provided are methods for detection of relative positions in space of particles, methods for generating matrices, methods for distance matrix determination, methods for identifying individual matrices and devices for recording and storing images of matrices.
摘要翻译： 本发明涉及用于组合固相合成，测定，功能蛋白质组学和诊断用途的珠粒或颗粒形式的空间编码聚合物基质。 还提供了这种珠粒或颗粒的组合物。 组合物的每个串珠聚合物基质包含多个空间固定的颗粒。 颗粒的空间固定赋予每个珠光聚合物基质上的“指纹”，其使得能够鉴定珠粒群体中独特的珠粒。 单个珠的独特鉴定使得在记录单个化学转化的同时执行组合化学策略成为可能。 还提供了用于检测颗粒空间中的相对位置的方法，用于生成矩阵的方法，用于距离矩阵确定的方法，用于识别各个矩阵的方法以及用于记录和存储矩阵的图像的设备。
-
公开(公告)号：US08754012B2
公开(公告)日：2014-06-17
申请号：US11661762
申请日：2005-09-05
申请人： Darren James Hart
发明人： Darren James Hart
CPC分类号： G01N33/6842 , C12N15/1034 , C12N15/1086 , G01N33/6803 , G01N2500/10
摘要： The present invention relates to methods of screening for expression of a soluble candidate protein within an expression library of candidate proteins. The method involves fusing each candidate protein in the library to a peptide substrate and identifying cells that express soluble candidate protein by detecting enzymatic modification of the peptide substrate.
摘要翻译： 本发明涉及在候选蛋白质的表达文库中筛选可溶性候选蛋白质的表达方法。 该方法包括将文库中的每个候选蛋白融合到肽底物，并通过检测肽底物的酶修饰鉴定表达可溶性候选蛋白的细胞。
-
-
-
-
-
-
-
-
-
搜索结果
124 条记录 (耗时 0.046 秒)
