公开(公告)号：US20200017817A1

公开(公告)日：2020-01-16

申请号：US16427952

申请日：2019-05-31

申请人： Berkeley Lights, Inc.

发明人： Darcy K. KELLY-GREENE ,  Russell A. Newstrom ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  C12M1/02

摘要： Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

公开(公告)号：US20200038857A1

公开(公告)日：2020-02-06

申请号：US16513445

申请日：2019-07-16

申请人： Berkeley Lights, Inc.

发明人： Andrew W. McFarland ,  Daniele Malleo ,  J. Tanner Nevill ,  Russell A. Newstrom ,  Keith J. Breinlinger ,  Paul M. Lundquist ,  Justin K. Valley ,  Jonathan Cloud Dragon Hubbard

IPC分类号： B01L3/00 ,  G02B21/08 ,  G02B27/14 ,  G01N27/447 ,  G02B21/26 ,  G02B21/32 ,  G02B21/36 ,  B01L9/00

摘要： A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.

公开(公告)号：US20230384573A1

公开(公告)日：2023-11-30

申请号：US18447583

申请日：2023-08-10

申请人： BERKELEY LIGHTS, INC.

发明人： Andrew W. McFarland ,  Daniele Malleo ,  J. Tanner Nevill ,  Russell A. Newstrom ,  Keith J. Breinlinger ,  Paul M. Lundquist ,  Justin K. Valley ,  Jonathan Cloud Dragon Hubbard

IPC分类号： G02B21/08 ,  G02B27/14 ,  B01L3/00 ,  B01L9/00 ,  G01N27/447 ,  G02B21/26 ,  G02B21/32 ,  G02B21/36

CPC分类号： G02B21/082 ,  G02B27/141 ,  B01L3/502715 ,  B01L9/527 ,  B01L3/50273 ,  G01N27/44721 ,  G01N27/44791 ,  G02B21/26 ,  G02B21/32 ,  G02B21/361 ,  G02B27/145 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0677 ,  G02B19/0028

摘要： A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.

公开(公告)号：US20160340632A1

公开(公告)日：2016-11-24

申请号：US15135252

申请日：2016-04-21

申请人： BERKELEY LIGHTS, INC.

发明人： Keith J. Breinlinger ,  Russell A. Newstrom ,  J. Tanner Nevill ,  Jason M. McEwen ,  David A. Weisbach

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12M3/06 ,  C12M3/00 ,  C12M1/04 ,  C12M1/26 ,  C12M1/12 ,  C12M1/24 ,  C12M1/02 ,  C12M1/36

CPC分类号： C12M23/58 ,  B01L3/502715 ,  B01L7/52 ,  C12M23/08 ,  C12M23/16 ,  C12M23/20 ,  C12M23/22 ,  C12M23/24 ,  C12M23/26 ,  C12M23/34 ,  C12M23/38 ,  C12M23/44 ,  C12M23/50 ,  C12M29/10 ,  C12M33/00 ,  C12M37/00 ,  C12M41/06 ,  C12M41/22 ,  C12M41/44 ,  C12M41/46 ,  C12M41/48

摘要： A station for culturing biological cells in a microfluidic device is provided. The station includes one or more thermally conductive mounting interfaces, each mounting interface configured for having a microfluidic device detachably mounted thereon; a thermal regulation system configured for controlling a temperature of microfluidic devices detachably mounted on the one or more mounting interfaces; and a media perfusion system configured to controllably and selectively dispense a flowable culturing media into microfluidic devices detachably mounted on the one or mounting interfaces.

摘要翻译： 提供了一种用于在微流体装置中培养生物细胞的站。 该工位包括一个或多个导热安装接口，每个安装接口构造成具有可拆卸地安装在其上的微流体装置; 热调节系统，被配置为控制可拆卸地安装在所述一个或多个安装界面上的微流体装置的温度; 以及媒体灌注系统，被配置为可控地和选择性地将可流动培养介质分配到可拆卸地安装在所述一个或安装界面上的微流体装置中。

公开(公告)号：US11492584B2

公开(公告)日：2022-11-08

申请号：US16537389

申请日：2019-08-09

申请人： Berkeley Lights, Inc.

发明人： Russell A. Newstrom ,  Andrew W. McFarland ,  Darcy K. Kelly-Greene ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/32 ,  C12M3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36

摘要： Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

公开(公告)号：US11434462B2

公开(公告)日：2022-09-06

申请号：US16427952

申请日：2019-05-31

申请人： Berkeley Lights, Inc.

发明人： Darcy K. Kelly-Greene ,  Russell A. Newstrom ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  C12M1/02

摘要： Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

公开(公告)号：US10407658B2

公开(公告)日：2019-09-10

申请号：US15282923

申请日：2016-09-30

申请人： BERKELEY LIGHTS, INC.

发明人： Russell A. Newstrom ,  Andrew W. McFarland ,  Darcy K. Kelly-Greene ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/02 ,  C12M1/32 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00 ,  C12M1/12

摘要： Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

公开(公告)号：US10384204B2

公开(公告)日：2019-08-20

申请号：US14963759

申请日：2015-12-09

申请人： Berkeley Lights, Inc.

发明人： Andrew W. McFarland ,  Daniele Malleo ,  J. Tanner Nevill ,  Russell A. Newstrom ,  Keith J. Breinlinger ,  Paul M. Lundquist ,  Justin K. Valley ,  Jonathan Cloud Dragon Hubbard

IPC分类号： B01L3/00 ,  B01L9/00 ,  G02B21/08 ,  G02B27/14 ,  G01N27/447 ,  G02B21/26 ,  G02B21/32 ,  G02B21/36 ,  G02B27/09 ,  G02B27/10 ,  G02B19/00

摘要： A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating subsystem, and which can be configured to supply light of substantially uniform intensity to the light modulating subsystem or directly to the optical train.

公开(公告)号：US20160193604A1

公开(公告)日：2016-07-07

申请号：US14963759

申请日：2015-12-09

申请人： Berkeley Lights, Inc.

发明人： Andrew W. McFarland ,  Daniele Malleo ,  J. Tanner Nevill ,  Russell A. Newstrom ,  Keith J. Breinlinger ,  Paul M. Lundquist ,  Justin K. Valley ,  Jonathan Cloud Dragon Hubbard

IPC分类号： B01L3/00 ,  G02B21/08 ,  G02B27/14 ,  G02B21/32 ,  G02B21/36 ,  G01N27/447 ,  G02B21/26

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/14 ,  B01L2300/06 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/12 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0677 ,  G01N27/44721 ,  G01N27/44791 ,  G02B19/0028 ,  G02B21/082 ,  G02B21/26 ,  G02B21/32 ,  G02B21/361 ,  G02B27/0994 ,  G02B27/1006 ,  G02B27/141 ,  G02B27/145

摘要： A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating subsystem, and which can be configured to supply light of substantially uniform intensity to the light modulating subsystem or directly to the optical train.

摘要翻译： 一种用于操作电动装置的系统包括被配置为保持和可操作地与电动装置耦合的支撑件;被配置为在电动装置中的一对电极上施加偏置电压的集成电信号产生子系统，以及光调制子系统， 将结构光发射到电动装置上。 该系统还可以包括热控流量控制器和/或被配置成测量电动装置上的阻抗。 该系统可以是光学显微镜，包括光学列车。 该系统还可以包括光管，其可以是光调制子系统的一部分，并且其可以被配置为将光强度基本上均匀的光提供给光调制子系统或者直接提供给光学列车。