公开(公告)号：US20130004946A1

公开(公告)日：2013-01-03

申请号：US13448290

申请日：2012-04-16

申请人： Jonathan Chesnut ,  Bhaskar Thyagarajan ,  Antje Taliana ,  Pauline Lieu ,  Mahendra Rao ,  Robert Bennett ,  Robert Burrier ,  Uma Lakshmipathy ,  Ying Liu

发明人： Jonathan Chesnut ,  Bhaskar Thyagarajan ,  Antje Taliana ,  Pauline Lieu ,  Mahendra Rao ,  Robert Bennett ,  Robert Burrier ,  Uma Lakshmipathy ,  Ying Liu

IPC分类号： C12N15/90 ,  C12Q1/68

CPC分类号： C12N15/1086 ,  C12N15/1082

摘要： The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification.

摘要翻译： 本公开一般涉及干细胞生物学，更具体地涉及干细胞的遗传操作。 公开了使用重组克隆技术的方法和组合物，其允许将复杂遗传构建体构建和插入胚胎和成体干细胞和祖细胞中。 所公开的方法将允许收获用整合位点预先设计的成体干细胞，以促进早期遗传修饰。

公开(公告)号：US20080216185A1

公开(公告)日：2008-09-04

申请号：US12016415

申请日：2008-01-18

申请人： Jonathan CHESNUT ,  Antje Taliana ,  Bhaskar Thyagarajan ,  Mahendra Rao ,  Pauline Lieu ,  Robert Bennett ,  Robert Burrier

发明人： Jonathan CHESNUT ,  Antje Taliana ,  Bhaskar Thyagarajan ,  Mahendra Rao ,  Pauline Lieu ,  Robert Bennett ,  Robert Burrier

IPC分类号： C12N15/00 ,  C12N15/87 ,  C12Q1/68

CPC分类号： C12N15/1086 ,  C12N15/1082

摘要： The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification.

摘要翻译： 本公开一般涉及干细胞生物学，更具体地涉及干细胞的遗传操作。 公开了使用重组克隆技术的方法和组合物，其允许将复杂遗传构建体构建和插入胚胎和成体干细胞和祖细胞中。 所公开的方法将允许收获用整合位点预先设计的成体干细胞，以促进早期遗传修饰。

公开(公告)号：US20130323770A1

公开(公告)日：2013-12-05

申请号：US13985559

申请日：2012-02-14

申请人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

发明人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC分类号： C12Q1/42

CPC分类号： G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

摘要： The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

摘要翻译： 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：US20050214940A1

公开(公告)日：2005-09-29

申请号：US10502224

申请日：2003-01-23

申请人： Mahendra Rao ,  Tahmina Mujtaba ,  Yuan Wu ,  Ying Liu

发明人： Mahendra Rao ,  Tahmina Mujtaba ,  Yuan Wu ,  Ying Liu

IPC分类号： A61K35/12 ,  C12N5/0797 ,  A61K45/00 ,  C12N5/08

CPC分类号： C12N5/0622 ,  A61K35/12 ,  A61K35/30 ,  C12N5/0623 ,  G01N33/56966

摘要： An isolated, pure homogeneous population of mammalian astrocyte retricted precursor cells which is CD44 immunoreactive and which generate astrocytes but not oligodendrocytes is provided. Methods for isolating and using these mammalian astrocyte restricted precursor cells are also provided.

摘要翻译： 提供了分离的，纯均质的哺乳动物星形胶质细胞前体细胞，其是CD44免疫反应性的并且产生星形胶质细胞而不是少突胶质细胞。 还提供了分离和使用这些哺乳动物星形胶质细胞限制性前体细胞的方法。

公开(公告)号：US20050125848A1

公开(公告)日：2005-06-09

申请号：US11036004

申请日：2005-01-14

申请人： Margot Mayer-Proschel ,  Mahendra Rao ,  Patrick Tresco ,  Darin Messina

发明人： Margot Mayer-Proschel ,  Mahendra Rao ,  Patrick Tresco ,  Darin Messina

IPC分类号： A61K35/12 ,  C12N5/0797 ,  A01K67/033 ,  C12N5/08 ,  A01K67/00

CPC分类号： A61K35/54 ,  A61K35/12 ,  C12N5/0623

摘要： A method for isolating human neuroepithelial precursor cells from human fetal tissue by culturing the human fetal cells in fibroblast growth factor and chick embryo extract and immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM is provided. In addition, methods for transplanting these cells into an animal are provided. Animals models transplanted with these human neuroepithelial precursor cells and methods for monitoring survival, proliferation, differentiation and migration of the cells in the animal model via detection of human specific markers are also provided.

公开(公告)号：US20050049706A1

公开(公告)日：2005-03-03

申请号：US10941620

申请日：2004-09-14

申请人： Darrel Brodke ,  Bret Berry ,  Ashok Khandkar ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

发明人： Darrel Brodke ,  Bret Berry ,  Ashok Khandkar ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

IPC分类号： A61F2/00 ,  A61F2/28 ,  A61F2/30 ,  A61F2/44 ,  A61F2/46 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/38 ,  A61L27/56

CPC分类号： A61L27/3804 ,  A61F2/30767 ,  A61F2/442 ,  A61F2/446 ,  A61F2/447 ,  A61F2/4611 ,  A61F2002/2835 ,  A61F2002/30011 ,  A61F2002/30016 ,  A61F2002/30064 ,  A61F2002/30153 ,  A61F2002/30158 ,  A61F2002/30166 ,  A61F2002/30179 ,  A61F2002/30224 ,  A61F2002/30235 ,  A61F2002/30261 ,  A61F2002/30266 ,  A61F2002/30271 ,  A61F2002/3028 ,  A61F2002/30329 ,  A61F2002/30616 ,  A61F2002/30677 ,  A61F2002/30733 ,  A61F2002/30774 ,  A61F2002/30777 ,  A61F2002/30789 ,  A61F2002/3082 ,  A61F2002/30822 ,  A61F2002/30827 ,  A61F2002/3085 ,  A61F2002/30881 ,  A61F2002/30891 ,  A61F2002/30892 ,  A61F2002/30896 ,  A61F2002/3092 ,  A61F2002/30968 ,  A61F2002/4475 ,  A61F2002/4623 ,  A61F2002/4627 ,  A61F2002/4629 ,  A61F2002/4648 ,  A61F2220/0025 ,  A61F2230/0019 ,  A61F2230/0026 ,  A61F2230/0028 ,  A61F2230/0058 ,  A61F2230/0063 ,  A61F2230/0069 ,  A61F2230/0082 ,  A61F2250/0019 ,  A61F2250/0023 ,  A61F2310/00203 ,  A61F2310/00239 ,  A61F2310/00796 ,  A61F2310/00928 ,  A61F2310/0097 ,  A61F2310/00976 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/3608 ,  A61L27/365 ,  A61L27/3847 ,  A61L27/56 ,  A61L2430/02 ,  A61L2430/38

摘要： An improved bone graft is provided for human implantation, particularly such as a spinal fusion cage for implantation into the inter-vertebral space between two adjacent vertebrae. The improved spinal fusion cage includes a substrate block of high strength biocompatible material having a selected size and shape to fit the anatomical space, and a controlled porosity analogous to natural bone. The substrate block may be coated with a bio-active surface coating material such as hydroxyapatite or a calcium phosphate to promote bone in growth and enhanced bone fusion. Upon implantation, the fusion cage provides a spacer element having a desired combination of mechanical strength together with osteoconductivity and osteoinductivity to promote bone ingrowth and fusion, as well as radiolucency for facilitated post-operative monitoring. The fusion cage may additionally carry one or more natural or synthetic therapeutic agents for further promoting bone ingrowth and fusion.

摘要翻译： 提供改进的骨移植物用于人类植入，特别是例如用于植入到两个相邻椎骨之间的椎间空间中的脊柱融合器。 改进的脊柱融合器包括具有选定尺寸和形状以适应解剖空间的高强度生物相容性材料的基底块，以及类似于天然骨的受控孔隙。 衬底块可以涂覆有生物活性表面涂层材料，例如羟基磷灰石或磷酸钙，以促进骨骼生长和增强骨融合。 植入后，融合笼提供了具有机械强度与骨传导性和骨诱导性的期望组合的间隔元件，以促进骨向内生长和融合，以及便于手术后监测的放射性。 融合笼可另外携带一种或多种天然或合成治疗剂，用于进一步促进骨向内生长和融合。

公开(公告)号：US20070166290A1

公开(公告)日：2007-07-19

申请号：US11694221

申请日：2007-03-30

申请人： Mahendra Rao ,  Mark Noble ,  Margot Mayer-Proschel

发明人： Mahendra Rao ,  Mark Noble ,  Margot Mayer-Proschel

IPC分类号： A61K35/30 ,  C12N5/08

CPC分类号： G01N33/5058 ,  A61K35/12 ,  C12N5/0622 ,  C12N5/0623 ,  C12N2501/135 ,  C12N2501/395 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00

摘要： A glial precursor cell population from mammalian central nervous system has been isolated. These A2B5+ E-NCAM− glial-restricted precursor (GRP) cells are capable of differentiating into oligodendrocytes, A2B5+ process-bearing astrocytes, and A2B5− fibroblast-like astrocytes, but not into neurons. GRP cells can be maintained by regeneration in culture. GRP cells differ from oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells in growth factor requirements, morphology, and progeny. Methods of use of GRP cells are also disclosed.

摘要翻译： 已经分离出来自哺乳动物中枢神经系统的胶质前体细胞群。 这些A2B5 +神经胶质细胞前体（GRP）细胞能够分化为少突胶质细胞，携带A2B5 +的星形胶质细胞， 和A2B5 成纤维细胞样星形胶质细胞，但不能进入神经元。 GRP细胞可通过培养中的再生来维持。 GRP细胞不同于少突胶质细胞-2型星形胶质细胞（O-2A）祖细胞的生长因子要求，形态和后代。 还公开了使用GRP细胞的方法。

公开(公告)号：US20060199264A1

公开(公告)日：2006-09-07

申请号：US11415056

申请日：2006-05-01

申请人： Mahendra Rao ,  Margot Mayer-Proschel

发明人： Mahendra Rao ,  Margot Mayer-Proschel

IPC分类号： C12N5/08

CPC分类号： C12N5/0661 ,  C12N5/0623 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2506/08

摘要： Multipotent neuroepithelial stem cells and lineage-restricted oligodendrocyte-astrocyte precursor cells are described. The neuroepithelial stem cells are capable of self-renewal and of differentiation into neurons, astrocytes, and oligodendrocytes. The oligodendrocyte-astrocyte precursor cells are derived from neuroepithelial stem cells, are capable of self-renewal, and can differentiate into oligodendrocytes and astrocytes, but not neurons. Methods of generating, isolating, and culturing such neuroepithelial stem cells and oligodendrocyte-astrocyte precursor cells are also disclosed.

公开(公告)号：US20050048041A1

公开(公告)日：2005-03-03

申请号：US10867628

申请日：2004-06-15

申请人： Mahendra Rao ,  Mario Capecchi

发明人： Mahendra Rao ,  Mario Capecchi

IPC分类号： C12N5/0797 ,  C12N15/90 ,  A61K48/00 ,  C12N5/08 ,  C12N9/10 ,  C12N15/85

CPC分类号： C12N5/0623 ,  C12N15/902 ,  C12N2510/04 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/60 ,  C12N2830/42 ,  C12N2840/203 ,  C12N2840/44

摘要： A method of obtaining and the resulting isolated progenitor or stem cell population of proliferating cells persistently expressing a candidate molecule. Further, novel methods of ex vivo gene product (e.g., protein) production and treating symptoms of neurological or neurodegenerative disorders are also provided.

摘要翻译： 获得和产生的增殖细胞的分离祖细胞或干细胞群的方法持续地表达候选分子。 此外，还提供了离体基因产物（例如蛋白质）生产和治疗神经或神经退行性疾病症状的新方法。

公开(公告)号：US09334523B2

公开(公告)日：2016-05-10

申请号：US13985559

申请日：2012-02-14

申请人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

发明人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC分类号： C12Q1/42 ,  G01N33/50 ,  G01N33/58

CPC分类号： G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

摘要： The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

摘要翻译： 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。