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公开(公告)号：US10435426B2
公开(公告)日：2019-10-08
申请号：US16198017
申请日：2018-11-21
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Youngsoon Um , Kyung Min Lee , Sun-Mi Lee , Hong Gon Kim , Gyeongtaek Gong
Abstract: The present disclosure relates to a method for separating sugars and acids with reduced energy consumption, including a step of diffusively dialyzing a first acid hydrolysate obtained by saccharifying biomass with an acid solution, thereby preparing a second acid hydrolysate wherein the concentration of the acid solution contained in the acid hydrolysate is decreased; and a step of electrolyzing the second acid hydrolysate, thereby separating sugars from the acid solution, which is advantageous in that less energy is consumed, the separated acid solution can be recycled directly without further treatment due to high concentration and loss of sugars can be minimized.
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公开(公告)号：US20240352401A1
公开(公告)日：2024-10-24
申请号：US18521797
申请日：2023-11-28
Applicant: Korea Institute of Science and Technology
Inventor： Sun Mi LEE , Jiwon Kim , Hyeoncheol Son , Ja Kyong Ko , Gyeongtaek Gong , Jung Ho Ahn , Youngsoon Um
CPC classification number: C12N1/165 , C12N9/0008 , C12N15/815 , C12R2001/645 , C12Y102/01003
Abstract: The present disclosure discloses a strain having resistance by neutralizing furfural, an inhibitor derived from lignocellulosic biomass, based on a technique using aldehyde dehydrogenase and a method of using the same. According to the present disclosure, furfural, an inhibitor derived from lignocellulosic biomass, is neutralized based on a technique using aldehyde dehydrogenase, so that a resistant strain overexpresses aldehyde dehydrogenase (EcAldH) in Yarrowia lipolytica, which has the ability to produce yeast oil, and has resistance to furfural, a biomass-derived inhibitor. Accordingly, even in the medium containing furfural, it can provide excellent strain growth and yeast oil production ability.
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公开(公告)号：US10975448B2
公开(公告)日：2021-04-13
申请号：US16253581
申请日：2019-01-22
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Youngsoon Um , Joongsuk Kim , Sun-Mi Lee , Gyeongtaek Gong
Abstract: The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.
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4.发明授权Paenibacillus sp. CAA11 capable of saccharification and fermentation of cellulose and transformed strain thereof 有权Paenibacillus sp。 能够糖化和发酵纤维素及其转化菌株的CAA11公开(公告)号：US09574214B2
公开(公告)日：2017-02-21
申请号：US15088935
申请日：2016-04-01
Applicant: Korea Institute Of Science And Technology
Inventor： Youngsoon Um , Han Min Woo , Gyeongtaek Gong , Eun Sook Kim , Sukhyeong Cho
IPC: C12P7/06 , C12N9/24 , C12P7/56 , C12R1/01 , C12N1/20 , C12N1/22 , C12P7/54 , C12P7/40 , C12P7/10 , C12N9/42
CPC classification number: C12P7/56 , C12N1/20 , C12N1/22 , C12N9/2437 , C12P7/065 , C12P7/10 , C12P7/40 , C12P7/54 , C12P19/02 , C12P19/12 , C12R1/01 , C12Y302/01004 , Y02E50/16 , Y02E50/17
Abstract: The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry.
Abstract translation: 本公开涉及能够糖化和发酵生物质衍生的纤维素的新菌株及其具有改善的生物质糖化能力的重组菌株。 本公开还涉及使用该菌株或重组菌株生产可用作生物能源材料如乙醇，乙酸，甲酸等的材料的方法。 菌株或重组菌株可用于生物能源工业。
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公开(公告)号：US20200149071A1
公开(公告)日：2020-05-14
申请号：US16551335
申请日：2019-08-26
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Sun Mi Lee , Phuong Nguyen Hoang Tran , Youngsoon Um , Gyeongtaek Gong , Ja Kyong Ko , Yeonjung Lee
IPC: C12N15/90 , C12N9/92 , C12N9/12 , C12N9/10 , C12P7/06 , C12N15/81 , C12N1/16 , C12N15/11 , C12N9/22 , C12N9/04 , C12N9/02
Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.
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Patent Agency Ranking
公开(公告)号：US10155964B2
公开(公告)日：2018-12-18
申请号：US15357815
申请日：2016-11-21
Applicant: Korea Institute of Science and Technology
Inventor： Han Min Woo , Sun-young Choi , Youngsoon Um , Gyeong Taek Gong , Sun Mi Lee , Yunje Kim
Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.
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公开(公告)号：US09719095B2
公开(公告)日：2017-08-01
申请号：US14950425
申请日：2015-11-24
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Han Min Woo , Eun Mi Kim , Youngsoon Um , Gyeongtaek Gong , Sun Mi Lee , Yunje Kim
IPC: C12N15/52 , C12N15/72 , C12N15/71 , C12N9/10 , C12N9/88 , C12N9/04 , C12N9/12 , C12N9/90 , C12N15/70
CPC classification number: C12N15/52 , C12N9/0006 , C12N9/1029 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/70 , C12N15/71 , C12N15/72 , C12Y101/01034 , C12Y203/01026 , C12Y205/01 , C12Y207/01036 , C12Y207/04002 , C12Y401/01033 , C12Y401/03 , C12Y503/03002
Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.
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公开(公告)号：US10087471B2
公开(公告)日：2018-10-02
申请号：US15092829
申请日：2016-04-07
Applicant: Korea Institute of Science and Technology
Inventor： Youngsoon Um , Yunje Kim , Kyung Min Lee , Han Min Woo , Gyeongtaek Gong , Ki Yeon Kim
Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.
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公开(公告)号：US09719097B2
公开(公告)日：2017-08-01
申请号：US14860373
申请日：2015-09-21
Applicant: Korea Institute of Science and Technology
Inventor： Han Min Woo , Jun Won Chwa , Youngsoon Um , Gyeong Taek Gong , Yunje Kim
CPC classification number: C12N15/74
Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.
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10.发明授权公开(公告)号：US09657318B2
公开(公告)日：2017-05-23
申请号：US14874040
申请日：2015-10-02
Applicant: Korea Institute of Science and Technology
Inventor： Youngsoon Um , Yunje Kim , Dongjin Suh , Kyung Min Lee , Jeong Myeong Ha
CPC classification number: C12P7/52 , C12P7/10 , C12P7/16 , C12P7/64 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/343
Abstract: Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.
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