利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

59 条记录 (耗时 0.039 秒)

    Cellulase variants
    2.
    发明授权
    Cellulase variants 有权
    纤维素酶变体

    公开(公告)号：US08017372B2

    公开(公告)日：2011-09-13

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen, legal representative ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42 ,  C12N15/00 ,  C12P21/04 ,  C07H21/04

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    CELLULASE VARIANTS
    3.
    发明申请
    CELLULASE VARIANTS 有权
    纤维素酶变体

    公开(公告)号：US20090170747A1

    公开(公告)日：2009-07-02

    申请号：US12394202

    申请日：2009-02-27

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christensen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christensen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C11D3/386 ,  C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase Variants
    4.
    发明申请
    Cellulase Variants 有权
    纤维素酶变体

    公开(公告)号：US20080206836A1

    公开(公告)日：2008-08-28

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase variants
    5.
    发明申请
    Cellulase variants 审中-公开
    纤维素酶变体

    公开(公告)号：US20050009166A1

    公开(公告)日：2005-01-13

    申请号：US10919195

    申请日：2004-08-16

    申请人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/15 ,  C12N1/21 ,  C12N9/42 ,  C12R1/645 ,  C12S11/00 ,  C12S99/00 ,  D06L4/40 ,  D06M16/00 ,  D21B1/02 ,  D21C5/02 ,  D21H21/24 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Alkaline cellulases
    7.
    发明授权
    Alkaline cellulases 失效
    碱性纤维素酶

    公开(公告)号：US5912157A

    公开(公告)日：1999-06-15

    申请号：US709979

    申请日：1996-09-09

    申请人： Claus von der Osten ,  Martin Schulein

    发明人： Claus von der Osten ,  Martin Schulein

    IPC分类号： C11D3/386 ,  C11D11/00 ,  C12N9/42 ,  C12S9/00 ,  C12S11/00 ,  D06M16/00 ,  D06P5/02 ,  D06P5/13 ,  D06P5/15 ,  D21C5/00

    CPC分类号： D21C5/005 ,  C11D3/386 ,  C12N9/2437 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/137 ,  D06P5/158

    摘要： The present invention relates to cellulases derived from the strain Myceliophthora thermophila and variants thereof comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having a substrate binding cleft of a depth of at least 12 .ANG., exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.

    摘要翻译： 本发明涉及源自嗜热丝菌嗜热链霉菌菌株的纤维素酶及其变体，其包含核心和任选的由最多10个氨基酸组成的C-末端连接体，特别是具有至少12个神经元深度的底物结合裂缝的纤维素酶， 在碱性pH范围内表现出增强的酶活性，同时在纤维素基质上施加中等的纤维素分解作用，并且例如可用于洗涤剂组合物，特别是用于去除污垢或着色澄清或防止背染; 织物柔软剂; 用于纺织品的生物抛光; 用于纸浆排水改良; 用于脱墨的旧纸; 用于植物降解。

    Extracellular expression of cellulose binding domains (CBD) using Bacillus
    10.
    发明授权
    Extracellular expression of cellulose binding domains (CBD) using Bacillus 失效
    使用芽孢杆菌的纤维素结合结构域（CBD）的细胞外表达

    公开(公告)号：US6060274A

    公开(公告)日：2000-05-09

    申请号：US959212

    申请日：1997-10-28

    申请人： Mads Eskelund Bjornvad ,  Martin Schulein ,  Per Lina Jorgensen

    发明人： Mads Eskelund Bjornvad ,  Martin Schulein ,  Per Lina Jorgensen

    IPC分类号： C12N15/09 ,  C07K14/195 ,  C07K14/21 ,  C07K14/33 ,  C12N1/21 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/07 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K14/195 ,  C07K14/21 ,  C07K14/33

    摘要： The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.

    摘要翻译： 本发明涉及用包含编码纤维素结合结构域（CBD）并能够表达所述序列的DNA序列的载体转化的芽孢杆菌宿主，所述表达的多肽蛋白质基本上由一个或多个非催化结构域组成; 所述纤维素结合结构域具有4kD至35kD的分子量，可从微生物或植物，优选来自细菌或真菌得到; 芽孢杆菌宿主例如 是枯草芽孢杆菌，地衣芽孢杆菌，巨大芽孢杆菌，嗜热脂肪芽孢杆菌和解淀粉芽孢杆菌之一; 和携带编码纤维素结合结构域的插入DNA序列的芽孢杆菌表达载体; 以及在芽孢杆菌宿主细胞中生产纤维素结合结构域多肽的方法。