公开(公告)号：US20210154683A1

公开(公告)日：2021-05-27

申请号：US17169030

申请日：2021-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Rui ZHENG ,  Sean MCCUSKER

IPC: B04B7/08 ,  B04B5/04 ,  B01D21/26

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

公开(公告)号：US20170269109A1

公开(公告)日：2017-09-21

申请号：US15392372

申请日：2016-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Maximilian CARPINO ,  Ray WHEELING ,  Nicholas Peter BAJKA

IPC: G01N35/00 ,  G01N35/10

CPC classification number: G01N35/0098 ,  G01N35/0099 ,  G01N35/10 ,  G01N35/1009 ,  Y10T436/255

Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.

公开(公告)号：US20160202209A1

公开(公告)日：2016-07-14

申请号：US15040815

申请日：2016-02-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  John F. Davidson ,  Wolfgang Hinz ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：US20140217477A1

公开(公告)日：2014-08-07

申请号：US14244849

申请日：2014-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  John F. DAVIDSON ,  Wolfgang HINZ ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：US20170038334A1

公开(公告)日：2017-02-09

申请号：US15331779

申请日：2016-10-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  John DAVIDSON ,  Wolfgang HINZ ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/447 ,  C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：US20160230214A1

公开(公告)日：2016-08-11

申请号：US15133428

申请日：2016-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

IPC: C12Q1/68 ,  B04B5/04

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。

公开(公告)号：US20210139949A1

公开(公告)日：2021-05-13

申请号：US17027206

申请日：2020-09-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

IPC: C12Q1/6806 ,  B04B13/00 ,  B04B5/04 ,  G01N35/00 ,  G01N35/10 ,  C12Q1/6869

Abstract: A sample preparation apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. In addition, the sample preparation apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles, as well as a centrifuge for loading such particles onto a sensor array. The sample preparation apparatus can further include receptacles for holding containers of reagent solutions.

公开(公告)号：US20190099765A1

公开(公告)日：2019-04-04

申请号：US16207522

申请日：2018-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Rui ZHENG ,  Sean MCCUSKER

IPC: B04B7/08 ,  B01D21/26 ,  B04B5/04 ,  G01N35/00 ,  G01N35/04 ,  B04B11/04

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

公开(公告)号：US20150133283A1

公开(公告)日：2015-05-14

申请号：US14536489

申请日：2014-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Rui ZHENG ,  Sean MCCUSKER

IPC: B04B7/08 ,  B01D21/26

CPC classification number: B04B7/08 ,  B01D21/262 ,  B04B5/0421 ,  B04B2011/046 ,  G01N2035/00158 ,  G01N2035/00495 ,  G01N2035/0449

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Abstract translation: 转子组件包括围绕第一轴线旋转的转子板; 铲斗，可旋转地安装在转子板上并围绕第二轴旋转; 以及止动板，其在打开位置和关闭位置之间相对于所述转子板绕所述第一轴线旋转，当处于所述关闭位置时，所述止动板与所述铲斗接合以将所述铲斗的角位置相对于平面固定 的转子组件的旋转。

公开(公告)号：US20130288873A1

公开(公告)日：2013-10-31

申请号：US13828633

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Ryan Jones ,  Sean McCusker ,  Maximilan Carpino ,  John Leamon ,  Jonathan Schultz ,  Jon A. Hoshizaki

IPC: B04B13/00

CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。