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21 条记录 (耗时 0.042 秒)

    Methods for purification of viruses
    1.
    发明授权
    Methods for purification of viruses 有权
    病毒纯化方法

    公开(公告)号：US08785173B2

    公开(公告)日：2014-07-22

    申请号：US13120638

    申请日：2009-09-24

    申请人： Mark Thompson ,  Janice Wee ,  Akanksha Nagpal

    发明人： Mark Thompson ,  Janice Wee ,  Akanksha Nagpal

    IPC分类号： C12N7/02

    CPC分类号： C12N7/00 ,  A61K2039/5254 ,  C12N2760/16051 ,  C12N2760/18551

    摘要： The present invention provides methods for the purification of cell-associated viruses from adherent cells (e.g., MDCK or Vero cells). In particular, the present invention provides purification methods for the production of immunogenic compositions comprising a live attenuated cell-associated virus (e.g., an attenuated respiratory syncytial virus (RSV) or cold-adapted, and/or temperature sensitive influenza virus) that result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.

    摘要翻译： 本发明提供了用于从贴壁细胞（例如MDCK或Vero细胞）纯化细胞相关病毒的方法。 特别地，本发明提供用于产生免疫原性组合物的纯化方法，其包含活减毒细胞相关病毒（例如，减毒呼吸道合胞病毒（RSV）或冷适应性和/或温度敏感性流感病毒），其导致 宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如Benzonase）的水平低于监管机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。

    METHODS FOR PURIFICATION OF VIRUSES
    2.
    发明申请
    METHODS FOR PURIFICATION OF VIRUSES 有权
    病毒纯化方法

    公开(公告)号：US20120219588A1

    公开(公告)日：2012-08-30

    申请号：US13120638

    申请日：2009-09-24

    申请人： Mark Thompson ,  Janice Wee ,  Akanksha Nagpal

    发明人： Mark Thompson ,  Janice Wee ,  Akanksha Nagpal

    IPC分类号： A61K39/155 ,  A61P31/14 ,  A61P37/04 ,  C12N7/02 ,  C12N7/00

    CPC分类号： C12N7/00 ,  A61K2039/5254 ,  C12N2760/16051 ,  C12N2760/18551

    摘要： The present invention provides methods for the purification of cell-associated viruses from adherent cells (e.g., MDCK or Vero cells). In particular, the present invention provides purification methods for the production of immunogenic compositions comprising a live attenuated cell-associated virus (e.g., an attenuated respiratory syncytial virus (RSV) or cold-adapted, and/or temperature sensitive influenza virus) that result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.

    摘要翻译： 本发明提供了用于从贴壁细胞（例如MDCK或Vero细胞）纯化细胞相关病毒的方法。 特别地，本发明提供用于产生免疫原性组合物的纯化方法，其包含活减毒细胞相关病毒（例如，减毒呼吸道合胞病毒（RSV）或冷适应性和/或温度敏感性流感病毒），其导致 宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如Benzonase）的水平低于管理机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。

    Tailored Oils Produced from Recombinant Oleaginous Microorganisms
    7.
    发明申请
    Tailored Oils Produced from Recombinant Oleaginous Microorganisms 有权
    由重组油脂微生物产生的定型油

    公开(公告)号：US20120203018A1

    公开(公告)日：2012-08-09

    申请号：US13365253

    申请日：2012-02-02

    申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

    发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

    IPC分类号： C12P7/64 ,  C07C57/02 ,  C12N1/13

    CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

    摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

    摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。