公开(公告)号：US20180245075A1

公开(公告)日：2018-08-30

申请号：US15889368

申请日：2018-02-06

Applicant: Trustees of Boston University

Inventor： Ahmad S. KHALIL ,  Albert J. KEUNG ,  Minhee PARK

IPC: C12N15/11 ,  C12N9/10 ,  C12N9/22

CPC classification number: C12N15/11 ,  C07K2319/81 ,  C12N9/1007 ,  C12N9/22 ,  C12N15/102 ,  C12N15/63 ,  C12N2310/20 ,  C12Y201/01 ,  C12Y301/21003

Abstract: Provided herein are engineered systems and methods for establishing DNA adenine methylation at specific genomic locations and using DNA adenine methylation as an artificial chemical “handle” on the genome. These systems and methods allow for placing the handle on specific genomic locations as well as molecular technologies to bind, spatially spread, and maintain the handle. The systems described herein comprise, in some embodiments, three functional modules that mediate m6A operations: (1) a synthetic initiator module to place m6A at specific genomic sites; (2) a synthetic readout module to program m6A recognition and m6A-dependent transcriptional logic; and (3) propagation module that implements “read-write,” a mechanism proposed to underlie chromatin spreading and epigenetic maintenance across cellular systems.

公开(公告)号：US20170369855A1

公开(公告)日：2017-12-28

申请号：US15539256

申请日：2015-12-24

Applicant: DANA-FARBER CANCER INSTITUTE, INC. ,  The Johns Hopkins University

Inventor： Carl NOVINA ,  Glenna MEISTER ,  Marc OSTERMEIER ,  Tina XIONG

IPC: C12N9/10 ,  C12Q1/68 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20

CPC classification number: C12N9/1007 ,  A61K48/00 ,  C07K2319/09 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/24 ,  C12N2800/40 ,  C12Q1/6897 ,  C12Q2600/154 ,  C12Y201/01 ,  C12Y201/01037 ,  C12Y301/00

Abstract: The present invention provides methods of systems and methods of site specific methylation.

公开(公告)号：US20160304885A1

公开(公告)日：2016-10-20

申请号：US15101078

申请日：2013-12-02

Applicant: CAMPAGNIE GERVAIS DANONE ,  UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK

Inventor： Douwe van Sinderen ,  Mary O'Connell Motherway ,  Debbie Watson ,  Tamara Smokvina ,  Peggy Garault

IPC: C12N15/74 ,  C12N1/20 ,  C12N9/10

CPC classification number: C12N15/746 ,  C12N1/20 ,  C12N9/1007 ,  C12Y201/01

Abstract: The present invention concerns a method for genetically transforming a Bifidobacterium strain comprising a step of methylation of a shuttle vector in an E. coli or a Gram-positive bacterium strain by two type II DNA methyltransferases from a Bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.

Abstract translation: 本发明涉及一种用于遗传转化双歧杆菌菌株的方法，其包括通过来自双歧杆菌的两种II型DNA甲基转移酶在大肠杆菌或革兰氏阳性菌株中甲基化穿梭载体的步骤：使腺嘌呤甲基化的甲基转移酶 核苷酸序列RTCAGG的第4位的碱基和甲基化核苷酸序列GGWCC的第4位的胞嘧啶碱基的甲基转移酶。 本发明还涉及可用于实施所述方法的遗传工具和培养基。

公开(公告)号：US20160194657A1

公开(公告)日：2016-07-07

申请号：US14989606

申请日：2016-01-06

Applicant: Iowa State University Research Foundation, Inc.

Inventor： Madan K. Bhattacharyya ,  Prashant Singh ,  Sekhar Kambakam ,  Micheline Ngaki

IPC: C12N15/82 ,  C07K14/415 ,  C12N9/10 ,  C12Q1/68 ,  C12N9/06

CPC classification number: C12N15/8282 ,  C07K14/415 ,  C12N9/0032 ,  C12N9/1007 ,  C12N15/8283 ,  C12N15/8285 ,  C12N15/8286 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Y105/03011 ,  C12Y201/01 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention relates to a method of increasing plant resistance to plant pathogens, particularly Sudden Death Syndrome in soybean against Fusarium virguliforme, through the use of Fusarium resistance nucleic acid molecules isolated from Glycine max and Fusarium virguliforme. In these plants, at least one heterologous Fusarium resistance protein is introduced in comparison to the wild-type plants to confer resistance to plant pathogens. The invention relates to transgenic plants and/or plant cells having increased resistance to plant pathogens, to expression vectors, transformed plants and plant cells as well as the use of such plants in a plant breeding program.

Abstract translation: 本发明涉及通过使用从甘氨酸和镰刀菌分离的耐镰刀菌核酸分子，增加植物病原体的抗性，特别是大豆中针对镰孢霉的突发性死亡综合征的方法。 在这些植物中，与野生型植物相比，引入至少一种异源镰刀菌抗性蛋白质以赋予对植物病原体的抗性。 本发明涉及具有增加的对植物病原体的抗性，表达载体，转化的植物和植物细胞的转基因植物和/或植物细胞，以及在植物育种程序中使用这种植物。

公开(公告)号：US20160010076A1

公开(公告)日：2016-01-14

申请号：US14775869

申请日：2014-03-14

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: C12N9/96 ,  C07K14/195 ,  C12N9/16 ,  C07K14/005 ,  C12N9/10 ,  C12N9/02

CPC classification number: C12N15/907 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.

Abstract translation: 用于RNA引导靶向异源功能结构域（如转录激活子）到特定基因组基因座的方法和构建体。

公开(公告)号：US20150361470A1

公开(公告)日：2015-12-17

申请号：US14713662

申请日：2015-05-15

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： David H. Sherman ,  Michael Marie Kaufman-Schofield ,  Sunit Jain ,  Gregory Dick

IPC: C12P17/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12N9/10

CPC classification number: C12N9/1029 ,  C12N9/0006 ,  C12N9/0051 ,  C12N9/0095 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P17/185 ,  C12Y108/01007 ,  C12Y118/01002 ,  C12Y201/01 ,  C12Y203/01 ,  C12Y203/0104 ,  C12Y203/01187 ,  C12Y402/01001 ,  C12Y402/01059 ,  C12Y603/04015

Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Abstract translation: 本公开涉及来自非核糖体肽合成酶（NRPS）的药物及其类似物的生物合成途径。 本发明提供了用于合成来自NRPS的药物的方便的微生物宿主中的异源表达的多核苷酸序列，由这些多核苷酸编码的多肽，包含多核苷酸的表达载体，包含多核苷酸或表达载体的宿主细胞，以及包含 宿主细胞。 还提供了用于生产来自NRPS的药物ET-743的方法。

公开(公告)号：US20140303029A1

公开(公告)日：2014-10-09

申请号：US14354543

申请日：2012-10-31

Applicant: UNIVERSITY OF UTAH RESARCH FOUNDATION

Inventor： Orly Alter

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1137 ,  C12N2310/14 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158 ,  C12Y201/01 ,  C12Y207/11001 ,  G06F19/00 ,  G16H50/30 ,  Y02A90/26 ,  C12Q2537/165

Abstract: Methods and genetic sequences are described for use in determining the diagnosis, subtype, prognosis, and disease course of high-grade gliomas, such as glioblastoma multiforme. One such method includes determining increased expression of at least one gene on a chromosome segment in cells of the glioma, the segment being 17:57,851,812-17:57,973,757; 7:127,892,509-7:127,947,649; 12:33,854-12:264,310; or 19:33,329,393-19:35,322,055; and estimating, based on the expression, a predicted length of survival, a probability of survival, or a predicted response to a therapy for the glioma.

Abstract translation: 描述了用于确定高级胶质瘤如多形性成胶质细胞瘤的诊断，亚型，预后和疾病过程的方法和基因序列。 一种这样的方法包括确定胶质瘤细胞中染色体片段上至少一个基因的表达增加，该片段为17：57,851,812-17：57,973,757; 7：127,892,509-7：127,947,649; 12：33,854-12：264,310; 或19：33,329,393-19：35,322,055; 并且基于表达来估计预测的存活时间长度，存活概率或对于胶质瘤治疗的预测响应。

公开(公告)号：US08835154B2

公开(公告)日：2014-09-16

申请号：US13980137

申请日：2012-01-18

Applicant: Kwang Ho Lee ,  Keun Chul Lee ,  Seok Myung Lee ,  Young Bin Hwang

Inventor： Kwang Ho Lee ,  Keun Chul Lee ,  Seok Myung Lee ,  Young Bin Hwang

IPC: C12N1/20 ,  C12N1/21 ,  C12P1/04 ,  C12P13/22 ,  C12P13/08 ,  C12N9/12 ,  C07K14/245 ,  C12N15/70

CPC classification number: C12N15/70 ,  C07K14/245 ,  C12N9/1007 ,  C12N9/1205 ,  C12P13/04 ,  C12P13/08 ,  C12P13/227 ,  C12Y201/01 ,  C12Y207/01023

Abstract: A microorganism of the genus Escherichia having enhanced L-amino acid productivity, wherein the microorganism is transformed to have an enhanced NAD kinase activity and an inactivated activity of an enzyme having an amino acid sequence of SEQ ID NO: 2 encoded by tehB gene and a method for producing L-amino acids using the microorganism of the genus Escherichia.

Abstract translation: 具有增强的L-氨基酸生产力的埃希氏菌属的微生物，其中所述微生物被转化为具有增强的NAD激酶活性和具有由tehB基因编码的SEQ ID NO：2的氨基酸序列的酶和 使用埃希氏杆菌属微生物生产L-氨基酸的方法。

公开(公告)号：US20140024087A1

公开(公告)日：2014-01-23

申请号：US13980137

申请日：2012-01-18

Applicant: Kwang Ho Lee ,  Keun Chul Lee ,  Seok Myung Lee ,  Young Bin Hwang

Inventor： Kwang Ho Lee ,  Keun Chul Lee ,  Seok Myung Lee ,  Young Bin Hwang

IPC: C12P13/22 ,  C12P13/08 ,  C12N15/70

CPC classification number: C12N15/70 ,  C07K14/245 ,  C12N9/1007 ,  C12N9/1205 ,  C12P13/04 ,  C12P13/08 ,  C12P13/227 ,  C12Y201/01 ,  C12Y207/01023

Abstract: A microorganism of the genus Escherichia having enhanced L-amino acid productivity, wherein the microorganism is transformed to have an enhanced NAD kinase activity and an inactivated activity of an enzyme having an amino acid sequence of SEQ ID NO: 2 encoded by tehB gene and a method for producing L-amino acids using the microorganism of the genus Escherichia.

Abstract translation: 具有增强的L-氨基酸生产力的埃希氏菌属的微生物，其中所述微生物被转化为具有增强的NAD激酶活性和具有由tehB基因编码的SEQ ID NO：2的氨基酸序列的酶和 使用埃希氏杆菌属微生物生产L-氨基酸的方法。

公开(公告)号：US20240052379A1

公开(公告)日：2024-02-15

申请号：US18346715

申请日：2023-07-03

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y101/01244 ,  C12Y101/02007 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.