公开(公告)号：US20220412890A1

公开(公告)日：2022-12-29

申请号：US17825222

申请日：2022-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin MAHER ,  Mingsong CHEN ,  Jacob K. FREUDENTHAL ,  Kuan Moon BOO ,  Soo Yong LAU ,  David FORTESCUE ,  Ming SHEN ,  Woon Liang Terence SOH

IPC: G01N21/64

Abstract: An instrument for processing and/or measuring a biological process comprises a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.

公开(公告)号：US11513133B2

公开(公告)日：2022-11-29

申请号：US16194187

申请日：2018-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher Barbee ,  Maximilian Carpino ,  Ray Wheeling ,  Nicholas Bajka

IPC: G01N35/00 ,  G01N35/10

Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.

公开(公告)号：US20220372435A1

公开(公告)日：2022-11-24

申请号：US17761879

申请日：2020-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS

IPC: C12N5/00

Abstract: Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.

公开(公告)号：US11505827B2

公开(公告)日：2022-11-22

申请号：US16351456

申请日：2019-03-12

Applicant: Roche Sequencing Solutions, Inc. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12M1/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

公开(公告)号：US20220349807A1

公开(公告)日：2022-11-03

申请号：US17729440

申请日：2022-04-26

Applicant: Life Technologies Corporation

Inventor： Eric Finan ,  Michael Ward

IPC: G01N15/14

Abstract: Provided are, inter alia, flow centration components that can be used in flow cytometers and other applications. A flow centrator can define central axis, a proximal end, and a distal end, and having a central bore extending within the flow centration component in the direction of the central axis; the flow centration component defining a splined outer surface that defines a plurality of circumferentially arranged bypass flute channels, the plurality of bypass flute channels extending in the direction of the central axis, and each of the bypass flute channels having a depth and a length. Also provided are related methods that utilize the disclosed components.

公开(公告)号：US20220348964A1

公开(公告)日：2022-11-03

申请号：US17860833

申请日：2022-07-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xavier de MOLLERAT du JEU

IPC: C12N15/86 ,  C12N7/00

Abstract: A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.

公开(公告)号：US11479814B2

公开(公告)日：2022-10-25

申请号：US16866211

申请日：2020-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/68 ,  C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US11474070B2

公开(公告)日：2022-10-18

申请号：US16196502

申请日：2018-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora ,  Melville Davey ,  Christian Koller ,  Simon Cawley ,  Alan Williams ,  David Kulp

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869 ,  G01N27/27 ,  G16B30/20

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

公开(公告)号：US11473132B2

公开(公告)日：2022-10-18

申请号：US16948442

申请日：2020-09-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

公开(公告)号：US11467092B2

公开(公告)日：2022-10-11

申请号：US16797561

申请日：2020-02-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mingsong Chen ,  Mauro Aguanno ,  Kuan Moon Boo ,  Woon Liang Terence Soh ,  Jacob K. Freudenthal ,  Jeffrey A. Marks

IPC: G01N21/64 ,  G01N21/27 ,  G01N21/03

Abstract: An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.