公开(公告)号：US11890321B2

公开(公告)日：2024-02-06

申请号：US17203640

申请日：2021-03-16

Applicant: President and Fellows of Harvard College ,  The Brigham and Women's Hospital, Inc.

Inventor： Ornella Barrandon ,  Tommaso Poggioli ,  Douglas A. Melton ,  Richard T. Lee

IPC: C07K14/475 ,  A61K38/18 ,  C07K14/00 ,  A61P3/04 ,  A61P3/06 ,  A61P3/10 ,  A61K38/19 ,  C12N5/00

CPC classification number: A61K38/1841 ,  A61K38/18 ,  A61K38/19 ,  A61P3/04 ,  A61P3/06 ,  A61P3/10 ,  C07K14/00 ,  C07K14/475 ,  C12N5/00

Abstract: Disclosed herein are a means to prevent and/or ameliorate age, disease and obesity associated metabolic diseases, such as diabetes and impaired glucose tolerance. Also disclosed are compositions and methods that relate to the findings that GDF11 prevents weight gain, improves glucose tolerance and reduces hepatosteatosis in aged mice administered a high fat diet. In particular, the methods and compositions described herein relate to increasing the level of GDF11 in a subject, thereby treating or preventing the development of obesity in the subject, reducing the metabolic consequences of obesity and improving the subject's metabolic health.

公开(公告)号：US20240035007A1

公开(公告)日：2024-02-01

申请号：US18089023

申请日：2022-12-27

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  University of Tokyo ,  President and Fellows of Harvard College

Inventor： Silvana KONERMANN ,  Alexandro TREVINO ,  Mark BRIGHAM ,  Fei RAN ,  Patrick HSU ,  Chie-yu LIN ,  Osamu NUREKI ,  Hiroshi NISHIMASU ,  Ryuichiro ISHITANI ,  Feng ZHANG

IPC: C12N9/22 ,  C12N15/113 ,  A61K48/00 ,  C12N15/90 ,  A61K38/46 ,  C12Q1/6876

CPC classification number: C12N9/22 ,  C12N15/113 ,  A61K48/005 ,  C12N15/907 ,  C12N2310/20 ,  C12N15/902 ,  C12Q1/6876 ,  C12Y301/00 ,  A61K38/465

Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.

公开(公告)号：US20240034765A1

公开(公告)日：2024-02-01

申请号：US18265523

申请日：2021-12-06

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael E. GREENBERG ,  Ee-Lynn YAP

IPC: C07K14/575 ,  C12N15/63 ,  G01N33/68 ,  A61P25/28

CPC classification number: C07K14/575 ,  C12N15/63 ,  G01N33/6896 ,  A61P25/28 ,  G01N2800/2814 ,  G01N2800/52

Abstract: The technology described herein is directed to pharmaceutical compositions comprising at least one Scg2 neuropeptide, as well as cell culture media or kits comprising such Scg2 neuropeptides. Also described herein are nucleic acids, vectors, or viral vectors encoding at least one Scg2 neuropeptide. In further aspects, described herein are methods of treating a memory-associated disorder, a learning disability, a neurodegenerative disease or disorder, or epilepsy with a pharmaceutical composition, nucleic acid, vector, or viral vector as described herein. In further aspects, described herein are detection methods of memory-associated analytes, such as Scg2 neuropeptides.

公开(公告)号：US20240024381A1

公开(公告)日：2024-01-25

申请号：US18178966

申请日：2023-03-06

Applicant: The Broad Institute, Inc. ,  The General Hospital Corporation ,  President and Fellows of Harvard College

Inventor： Douglas Kenny ,  Hera Vlamakis ,  Ramnik Xavier ,  Emily Balskus ,  Damian Plichta

IPC: A61K35/741 ,  A61P3/06 ,  A61K31/575 ,  A61K9/00 ,  C12N15/70 ,  C12N9/04 ,  C12Q1/26 ,  C12Q1/10

CPC classification number: A61K35/741 ,  A61P3/06 ,  A61K31/575 ,  A61K9/0053 ,  C12N15/70 ,  C12N9/0006 ,  C12Q1/26 ,  C12Q1/10 ,  C12Y101/03006 ,  G01N2800/50 ,  G01N2333/902 ,  G01N2800/044 ,  G01N2800/065

Abstract: Microbes expressing cholesterol oxidoreductase (COR) proteins, methods of engineering the microbes expressing COR proteins, compositions and methods of using the microbes are provided.

公开(公告)号：US20240018569A1

公开(公告)日：2024-01-18

申请号：US18459068

申请日：2023-08-31

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Je-Hyuk Lee ,  George M. Church ,  Michael Super

IPC: C12Q1/689 ,  G01N33/569 ,  G01N33/53 ,  C12Q1/6804

CPC classification number: C12Q1/689 ,  G01N33/56911 ,  G01N33/5308 ,  C12Q1/6804

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

公开(公告)号：US20240018544A1

公开(公告)日：2024-01-18

申请号：US18351800

申请日：2023-07-13

Applicant: The General Hospital Corporation ,  President and Fellows of Harvard College

Inventor： J. Keith Joung ,  Peter Cabeceiras

IPC: C12N15/86 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N2740/10023 ,  C12N2740/10042 ,  C12N2740/10052

Abstract: Human-derived virus-like particles (heVLPs), comprising a membrane comprising a phospholipid bilayer with one or more HERV-derived envelope proteins on the external side; one or more HERV-derived GAG proteins in the heVLP core, and a cargo molecule, e.g., a biomolecule and/or chemical cargo molecule, disposed in the core of the heVLP on the inside of the membrane, wherein the heVLP does not comprise a gag protein, except for gag proteins that are encoded in the human genome or gag proteins that are encoded by a consensus sequence that is derived from gag proteins found in the human genome, and methods of use thereof for delivery of the cargo molecule to cells.

公开(公告)号：US11859173B2

公开(公告)日：2024-01-02

申请号：US17366757

申请日：2021-07-02

Applicant: Northeastern University ,  President and Fellows of Harvard College

Inventor： Avinash M. Basavanna ,  Anna Duraj-Thatte ,  Neel S. Joshi

IPC: C12N1/20 ,  C12N1/18 ,  C12R1/19

CPC classification number: C12N1/20 ,  C12N1/18 ,  C12R2001/19

Abstract: Disclosed are biomaterials, including engineered living materials (ELMs), comprising a plurality of microbial cells, wherein the material has a Young's Modulus of at least 5 Gpa; and methods of fabricating said biomaterials.

公开(公告)号：US11850589B2

公开(公告)日：2023-12-26

申请号：US17848523

申请日：2022-06-24

Applicant: The Brigham and Women's Hospital, Inc. ,  President and Fellows of Harvard College ,  Vilnius University

Inventor： Joseph Italiano ,  Linas Mazutis ,  Jonathan N. Thon ,  David A. Weitz

IPC: B01L3/00 ,  G01N33/86 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/06 ,  G01N33/49

CPC classification number: B01L3/502753 ,  B01L3/502746 ,  B01L3/502776 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12M29/04 ,  C12M29/10 ,  C12M29/20 ,  C12M41/36 ,  C12M41/40 ,  G01N33/86 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2400/086 ,  G01N33/491 ,  G01N2800/222 ,  Y10T436/25375

Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.

公开(公告)号：US20230398729A1

公开(公告)日：2023-12-14

申请号：US18034756

申请日：2021-11-11

Applicant: President and Fellows of Harvard College

Inventor： Jochen MUELLER ,  Jennifer A. LEWIS ,  Katia BERTOLDI

IPC: B29C64/118 ,  B33Y70/00 ,  B33Y80/00

CPC classification number: B29C64/118 ,  B33Y70/00 ,  B33Y80/00

Abstract: A method of controlling macroscopic properties of a metamaterial includes 3D printing a lattice structure comprising interconnected struts, where each strut comprises one or more printed filaments. Each printed filament comprises an active material or a passive material, and the active material has a modulus with a higher stimulus dependence than that of the passive material. The printed filaments comprising the active material are disposed at predetermined regions of the lattice structure. After 3D printing, the lattice structure is exposed to a stimulus, and the predetermined regions comprising the active material soften or stiffen. Thus, the macroscopic properties of the lattice structure may be controlled.

公开(公告)号：US11834712B2

公开(公告)日：2023-12-05

申请号：US18177496

申请日：2023-03-02

Applicant: President and Fellows of Harvard College

Inventor： Xiaoliang Sunney Xie ,  Katsuyuki Shiroguchi ,  Peter A. Sims ,  Tony Z. Jia

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6853 ,  C12N2320/10 ,  C12Q1/6853 ,  C12Q2525/131 ,  C12Q2525/301 ,  C12Q2537/16 ,  C12Q2563/179

Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.