-
公开(公告)号：US09944980B2
公开(公告)日：2018-04-17
申请号：US11982465
申请日：2007-10-31
Applicant: David R. Rank , Jonas Korlach , Yue Xu , Stephen Turner , Jeffery Wegener , Daniel Roitman , John Lyle
Inventor： David R. Rank , Jonas Korlach , Yue Xu , Stephen Turner , Jeffery Wegener , Daniel Roitman , John Lyle
CPC classification number: C12Q1/6874 , B01J19/0046 , B01J2219/00427 , B01J2219/0045 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y20/00 , B82Y30/00 , C12Q1/6837 , Y10T29/49002 , C12Q2565/629 , C12Q2527/127
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
-
公开(公告)号：US09175341B2
公开(公告)日：2015-11-03
申请号：US12635618
申请日：2009-12-10
Applicant: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
Inventor： Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
-
公开(公告)号：US08846881B2
公开(公告)日：2014-09-30
申请号：US13552952
申请日：2012-07-19
Applicant: Jonas Korlach , Jeffrey Wegener
Inventor： Jonas Korlach , Jeffrey Wegener
CPC classification number: C12Q1/6869 , C07H19/10 , C12Q1/6818 , C12Q1/6874 , G01N33/5308
Abstract: Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types, through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.
Abstract translation: 核酸组合物，制备和使用这样的组合物的方法，所述组合物包含可被配置为通过可交换并优选非共价连接组分为不同核苷酸类型提供期望功能的模块化官能团。 这样的组合物可用于各种应用，包括核酸分析。
-
公开(公告)号：US08658364B2
公开(公告)日：2014-02-25
申请号：US13427725
申请日：2012-03-22
Applicant: Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
Inventor： Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
CPC classification number: C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301
Abstract: Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released.
Abstract translation: 提供了用于分离聚合酶 - 核酸复合物的组合物，方法和系统。 通过使用钩分子将复合物与游离酶分离以靶向核酸上的单链区域。 通过引发核酸合成，可以从具有活性和非活性复合物的混合物中分离活性配合物，以便打开一部分双链区域，使该区域成为单链。 钩状分子被靶向以结合由此暴露的序列。 分离与活性聚合酶 - 核酸复合物结合的钩分子，释放活性聚合酶 - 核酸复合物。
-
公开(公告)号：US08367813B2
公开(公告)日：2013-02-05
申请号：US12620293
申请日：2009-11-17
Applicant: Jonas Korlach
Inventor： Jonas Korlach
CPC classification number: C12Q1/6876 , C07H19/10 , C12Q1/6874 , G01N21/6486 , C12Q2565/629 , C12Q2563/155
Abstract: The present invention provides labeled phospholink nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyzes based thereon, including DNA sequencing, single base identification, hybridization assays, and others.
Abstract translation: 本发明提供了可以在模板定向核酸合成反应和其他核酸反应中使用的代替天然存在的三磷酸核苷酸或其他类似物的标记的磷脂酰核苷酸，并且基于此进行各种分析，包括DNA测序，单碱基鉴定，杂交测定， 和别的。
-
Patent Agency Ranking
公开(公告)号：US20130029853A1
公开(公告)日：2013-01-31
申请号：US13633673
申请日：2012-10-02
Applicant: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
Inventor： Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
-
97.发明授权Surface comprising DNA polymerase having mutations bound through an affinity tag 有权表面包含具有通过亲和标签结合的突变的DNA聚合酶公开(公告)号：US08323939B2
公开(公告)日：2012-12-04
申请号：US11978139
申请日：2007-10-26
Applicant: David Hanzel , Jonas Korlach , Paul Peluso , Geoffrey Otto , Thang Pham , David Rank , Stephen Turner
Inventor： David Hanzel , Jonas Korlach , Paul Peluso , Geoffrey Otto , Thang Pham , David Rank , Stephen Turner
CPC classification number: C12Q1/6837 , C07K2319/23 , C12N9/1252 , C12Q1/6844 , C12Q2521/101 , C12Q2565/531
Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
Abstract translation: 提供活性表面偶联的聚合酶，包括这种聚合酶的表面，以及制备和使用表面连接的聚合酶的方法。
-
98.发明申请公开(公告)号：US20120302459A1
公开(公告)日：2012-11-29
申请号：US13487978
申请日：2012-06-04
Applicant: David R. Rank , Jeffrey Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ron Cicero
Inventor： David R. Rank , Jeffrey Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ron Cicero
CPC classification number: C12Q1/6874 , B01J19/0046 , B01J2219/00427 , B01J2219/0045 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y20/00 , B82Y30/00 , C12Q1/6837 , Y10T29/49002 , C12Q2565/629 , C12Q2527/127
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
Abstract translation: 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。
-
公开(公告)号：US08133672B2
公开(公告)日：2012-03-13
申请号：US12414191
申请日：2009-03-30
Applicant: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
Inventor： Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
CPC classification number: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
-
公开(公告)号：US20110244447A1
公开(公告)日：2011-10-06
申请号：US13032478
申请日：2011-02-22
Applicant: Jonas Korlach
Inventor： Jonas Korlach
IPC: C12Q1/68
CPC classification number: C12N9/1252 , C07K2319/00 , C07K2319/22 , C07K2319/23 , C07K2319/30 , C07K2319/41 , C07K2319/42 , C07K2319/50 , C12Q1/6869 , C12Q2527/113 , C12Q2521/101 , C12Q2527/137 , C12Q2563/107 , C12Q2537/161 , C12Q2525/101
Abstract: Provided are methods and compositions for measuring the transient binding of nucleotides and nucleotide analogs under conditions where the nucleotides or nucleotide analogs are unincorporable. The transient binding can be determined under single molecule observation conditions providing information about the kinetics of nucleotide analog sampling of the active site of the enzyme. The methods can be used for polymerase enzyme development, mechanistic understanding, and drug discovery.
Abstract translation: 提供了在核苷酸或核苷酸类似物不可合并的条件下测量核苷酸和核苷酸类似物的瞬时结合的方法和组合物。 可以在单分子观察条件下确定瞬时结合，提供关于酶的活性位点的核苷酸类似物取样的动力学的信息。 该方法可用于聚合酶发酵，机理理解和药物发现。
-
-
-
-
-
-
-
-
-
Search Results
123
records
(took 0.041 seconds)
