公开(公告)号：US2927059A

公开(公告)日：1960-03-01

申请号：US59409156

申请日：1956-06-27

申请人： INT MINERALS & CHEM CORP

发明人： GOOD ROBERT C ,  GUNSALUS IRWIN C

IPC分类号： C12P13/14 ,  C12P13/20

CPC分类号： C12P13/14 ,  C12P13/20 ,  Y10S435/942 ,  Y10S435/944

公开(公告)号：US2773001A

公开(公告)日：1956-12-04

申请号：US43290754

申请日：1954-05-27

申请人： ROHM & HAAS

发明人： SMYTHE CARL V ,  HUANG HSING T

IPC分类号： C12P13/14

CPC分类号： C12P13/14 ,  Y10S435/94 ,  Y10S435/942 ,  Y10S435/944

公开(公告)号：US20240067998A1

公开(公告)日：2024-02-29

申请号：US18270491

申请日：2021-12-29

申请人： NINGXIA EPPEN BIOTECH CO., LTD

发明人： Houbo SU ,  Aiying WEI ,  Gang MENG ,  Lipeng YANG ,  Fengyong MA ,  Huiping JIA ,  Xiaoqun ZHOU ,  Chunguang ZHAO

IPC分类号： C12P13/14 ,  C07K14/34 ,  C12N15/77

CPC分类号： C12P13/14 ,  C07K14/34 ,  C12N15/77

摘要： Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.

公开(公告)号：US11913053B2

公开(公告)日：2024-02-27

申请号：US17277211

申请日：2019-09-17

申请人： Nanjing Bestzyme Bio-Engineering Co., Ltd.

发明人： Jie Lin ,  Hongxian Xu ,  Hui Peng

IPC分类号： C12P7/06 ,  C12P13/08 ,  C12P13/14

CPC分类号： C12P7/06 ,  C12P13/08 ,  C12P13/14 ,  C12Y302/01028

摘要： Provided is an application of trehalase in fermentative production. The trehalase has amino acid sequences shown in SEQ ID NO.6, SEQ ID NO.7, and SEQ ID NO.8. Provided are methods for producing and applying trehalase, particularly being applied in the production and fermentation of alcohol and an amino acid.

公开(公告)号：US20230416793A1

公开(公告)日：2023-12-28

申请号：US18307947

申请日：2023-04-27

申请人： Ajinomoto Co., Inc.

发明人： Seiko Hirano ,  Kota Inoue ,  Kazuyuki Hayashi ,  Kosuke Yokota ,  Mika Moriya ,  Tomoko Suzuki ,  Yoshitomo Kadokura ,  Takeshi Nagahiko ,  Akari Tashiro

IPC分类号： C12P13/14 ,  C12N15/77 ,  C12N9/12 ,  C12N9/16 ,  C12N9/02 ,  C12N9/10 ,  C12N9/04 ,  C12N9/88

CPC分类号： C12P13/14 ,  C12N15/77 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/0006 ,  C12N9/88 ,  C12R2001/15

摘要： Provided is a method of producing an L-amino acid such as L-glutamic acid and the like. An L-amino-acid is produced by cultivating a coryneform bacterium having L-amino acid-producing ability in a culture medium, which has been modified so as to have one or more of the following modifications: (A) a modification for increasing activity of acetate kinase, (B) a modification for increasing activity of fructose-1,6-bisphosphatase, (C) a modification for decreasing activity of pyruvate dehydrogenase, (D) a modification for decreasing activity of aspartate transaminase, and (E) a modification for decreasing activity of malic enzyme; and collecting the L-amino acid from the culture medium and/or the bacterial cells.

公开(公告)号：US20230323412A1

公开(公告)日：2023-10-12

申请号：US18040883

申请日：2021-01-04

申请人： NINGXIA EPPEN BIOTECH CO., LTD.

发明人： Gang MENG ,  Chunguang ZHAO ,  Aiying WEI ,  Xiaoqun ZHOU ,  Fengyong MA ,  Lipeng YANG ,  Houbo SU ,  Huiping JIA ,  Bin TIAN ,  Xiaowei GUO

IPC分类号： C12P13/08 ,  C12P13/14 ,  C12N15/77 ,  C07K14/34

CPC分类号： C12P13/08 ,  C12P13/14 ,  C12N15/77 ,  C07K14/34

摘要： Taking Corynebacterium glutamicum YP97158 as the starting bacterium, introducing site-directed mutation and/or expression enhancement in the coding region of its NCgl1089 gene, the coding region of NCgl0761 gene, and/or the coding region of ptsS gene, the obtained mutant gene and the recombination comprising said gene has high-efficiency L-amino acids production capacity, which greatly increases the output of L-amino acids, and the strain has good stability, which reduces the production cost as an L-amino acids production strain.

公开(公告)号：US11746345B2

公开(公告)日：2023-09-05

申请号：US15165606

申请日：2016-05-26

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova

IPC分类号： C12N9/00 ,  C12P13/08 ,  C12P13/04 ,  C12N15/52 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/22 ,  C12P13/24

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y603/02002

摘要： The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

公开(公告)号：US11453898B1

公开(公告)日：2022-09-27

申请号：US17566702

申请日：2021-12-31

申请人： Henan Julong Biological Engineering Co., Ltd ,  Tianjin University of Science and Technology

发明人： Xiaoguang Fan ,  Xiaodong Liu ,  Jing Li ,  Ning Chen ,  Bochao Liu ,  Shuai Liu ,  Chaochao Sun ,  Yongchao Liu ,  Jiajia Teng ,  Mengtao Zhang ,  Yuanqing Ji ,  Yuhang Zhou ,  Qingyang Xu

IPC分类号： C12P13/14 ,  C12N15/70 ,  C12N1/00

摘要： The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

公开(公告)号：US11198894B2

公开(公告)日：2021-12-14

申请号：US16268082

申请日：2019-02-05

申请人： AJINOMOTO CO., INC.

发明人： Kazuteru Yamada ,  Ye Zhang ,  Kenji Abe ,  Naoki Iwanaga ,  Ryo Takeshita ,  Yuri Uehara ,  Chika Hikichi ,  Yasuhiro Oota

IPC分类号： C12P13/20 ,  C12P13/08 ,  C12P13/04 ,  C12N15/09 ,  A23K50/80 ,  A23K10/18 ,  A01K61/10 ,  A01K61/59 ,  C12N1/20 ,  C12P13/14

摘要： A method for producing an L-amino acid such as L-lysine is provided. An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a carotenoid biosynthesis enzyme is increased, in a medium, and collecting the L-amino acid from the medium.

公开(公告)号：US11091744B2

公开(公告)日：2021-08-17

申请号：US16659813

申请日：2019-10-22

申请人： CJ CHEILJEDANG CORPORATION

发明人： Jae Hun Lee ,  Young Lyeol Yang ,  In Seok Oh

IPC分类号： C12N15/70 ,  C12N1/20 ,  C12N9/10 ,  C12P13/00 ,  C12P13/14

摘要： Provided are a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.