公开(公告)号：US10030267B2

公开(公告)日：2018-07-24

申请号：US15832183

申请日：2017-12-05

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180179580A1

公开(公告)日：2018-06-28

申请号：US15887711

申请日：2018-02-02

Applicant: 10X Genomics, Inc.

Inventor： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20180094314A1

公开(公告)日：2018-04-05

申请号：US15832183

申请日：2017-12-05

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180051321A1

公开(公告)日：2018-02-22

申请号：US15718893

申请日：2017-09-28

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12N15/1006 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20180015472A1

公开(公告)日：2018-01-18

申请号：US15717840

申请日：2017-09-27

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/10 ,  G01N35/00 ,  G01N35/08

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N35/085 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20170348691A1

公开(公告)日：2017-12-07

申请号：US15596754

申请日：2017-05-16

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/10 ,  G01N35/00 ,  G01N35/08

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N35/085 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20170342404A1

公开(公告)日：2017-11-30

申请号：US15470814

申请日：2017-03-27

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12N15/10 ,  C12Q1/68 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20170016041A1

公开(公告)日：2017-01-19

申请号：US15156628

申请日：2016-05-17

Applicant: 10X Genomics, Inc.

Inventor： Lawrence Greenfield ,  Christopher Hindson

IPC: C12P19/34

Abstract: The disclosure provides stabilized reducing agents and methods for using them in sample preparation. Stabilized reducing agents described herein provide easy-to-use replacement reducing agents for reducing agents that undergo side-reactions that can render them ineffective as reducing agents and/or decrease the concentration of available reducing agent. In some cases, a stabilized reducing agent is an activatable reducing agent that can be activated upon application of a stimulus to the reducing agent.

Abstract translation: 本公开提供了稳定的还原剂和在样品制备中使用它们的方法。 本文所述的稳定的还原剂提供易于使用的还原剂，其用于还原剂，所述还原剂经历可能使其作为还原剂无效的副反应和/或降低可用的还原剂的浓度。 在一些情况下，稳定的还原剂是可活化的还原剂，其可以在向还原剂施加刺激时被活化。

公开(公告)号：US20250067730A1

公开(公告)日：2025-02-27

申请号：US18932448

申请日：2024-10-30

Applicant: 10x Genomics, Inc.

Inventor： Andrew D. PRICE ,  Christopher Hindson

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

Abstract: The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

公开(公告)号：US12169198B2

公开(公告)日：2024-12-17

申请号：US18235616

申请日：2023-08-18

Applicant: 10X GENOMICS, INC.

Inventor： Andrew D. Price ,  Christopher Hindson

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58 ,  C40B30/04 ,  C40B70/00

Abstract: The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.