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174 条记录 (耗时 0.04 秒)

    MEANS AND METHODS FOR MANIPULATING SEQUENTIAL PHAGOLYSOMALCYTOSOLIC TRANSLOCATION OF MYCOBACTERIA, AND USES THEREOF
    112.
    发明申请
    MEANS AND METHODS FOR MANIPULATING SEQUENTIAL PHAGOLYSOMALCYTOSOLIC TRANSLOCATION OF MYCOBACTERIA, AND USES THEREOF 审中-公开
    用于处理MYCOBACTERIA的顺序性PHOMOYSOMALCYTOSOLIC转录的手段和方法及其用途

    公开(公告)号：US20090263418A1

    公开(公告)日：2009-10-22

    申请号：US12307948

    申请日：2007-06-29

    申请人： Nicole Neeltje Speelman-Van Der Wel ,  Michael Barry Brenner ,  Jacobus Peter Johannes Peters

    发明人： Nicole Neeltje Speelman-Van Der Wel ,  Michael Barry Brenner ,  Jacobus Peter Johannes Peters

    IPC分类号： A61K39/04 ,  C12Q1/68 ,  C12Q1/20 ,  C12N15/87 ,  C12N1/21

    CPC分类号： C12N1/36 ,  A61K39/04 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/35

    摘要： Mycobacteria such as M. tuberculosis and M. leprae are considered to be prototypical intracellular bacilli that have evolved strategies to enable growth in the intracellular phagosomes of the host cell. By contrast, we show that lysosomes rapidly fuse with the virulent M. tuberculosis and M. leprae— containing phagosomes of human monocyte-derived dendritic cells and macrophages. After 2 days, M. tuberculosis progressively translocate from phagolysosomes into the cytosol where they replicate. Cytosolic entry is also observed for M. leprae but not for the vaccine strain, M. bovis BCG, or killed mycobacteria, and is dependent upon secretion of the mycobacterial gene products CFP-IO and ESAT-6 of the RDI region. The present invention further provides means and methods for using these findings in therapeutic and immunogenic compositions.

    摘要翻译： 分枝杆菌如结核分枝杆菌和麻疯病被认为是原型细胞内杆菌，其具有促进宿主细胞的细胞内吞噬体生长的策略。 相比之下，我们显示溶酶体与人单核细胞衍生的树突状细胞和巨噬细胞的毒性结核分枝杆菌和含leprae的吞噬体快速融合。 2天后，结核分枝杆菌从吞噬体逐渐转移到细胞质中，在那里它们复制。 对于麻疯病菌也观察到胞质进入，但不是针对疫苗株，牛分枝杆菌BCG或杀死的分枝杆菌，并且依赖于RDI区域的分枝杆菌基因产物CFP-10和ESAT-6的分泌。 本发明还提供了在治疗和免疫原性组合物中使用这些发现的方法和方法。

    Electrochemical method for detecting hemoglobin or hematocrit and test strip thereof
    113.
    发明申请
    Electrochemical method for detecting hemoglobin or hematocrit and test strip thereof 有权
    用于检测血红蛋白或血细胞比容及其测试条的电化学方法

    公开(公告)号：US20090104641A1

    公开(公告)日：2009-04-23

    申请号：US12003564

    申请日：2007-12-28

    申请人： Chein-Shyong Su ,  Tai-Guang Wu

    发明人： Chein-Shyong Su ,  Tai-Guang Wu

    IPC分类号： C12Q1/20 ,  G01N33/72

    CPC分类号： G01N33/726

    摘要： The present invention provides an electrochemical method and a test strip for detecting hemoglobin in a specimen. The method includes the steps of providing the specimen with a reagent, which has a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by the reaction of the hemoglobin and the electron mediator in a specimen under a potentiostatic condition; and calculating a hemoglobin concentration in the specimen according to the detected electric current. In the method of the present invention, tetrathiafulvalene modified by cyclodextrin is used as an electron mediator, which directly interacts with the hemoglobin in the specimen. Since the hemoglobin concentration is in inverse proportion to the value of electric current or the value of SOC (state-of-charge) integrated by electric current value with time, the hemoglobin concentration in a whole blood specimen or the hematocrit level in a blood specimen is accurately and rapidly obtained according to the detected electric value or the value of SOC.

    摘要翻译： 本发明提供一种用于检测样品中的血红蛋白的电化学方法和测试条。 该方法包括以下步骤：向样品提供具有缓冲溶液，表面活性剂和电子介体的试剂，由环糊精改性的四硫富瓦烯; 在恒电位条件下检测样品中血红蛋白和电子介体的反应产生的电流; 并根据检测到的电流计算样品中的血红蛋白浓度。 在本发明的方法中，将通过环糊精改性的四硫富瓦烯用作电子介体，其直接与样品中的血红蛋白相互作用。 由于血红蛋白浓度与电流值或通过电流值随时间积分的SOC（充电状态）的值成反比，所以血样中的血红蛋白浓度或血样中的血细胞比容水平 根据检测到的电值或SOC的值准确而快速地获得。

    Two-photon probe for real-time monitoring of intracellular magnesium ions, method for preparing the two-photon probe and method for real-time monitoring of intracellular magnesium ions using the two-photon probe
    114.
    发明申请
    Two-photon probe for real-time monitoring of intracellular magnesium ions, method for preparing the two-photon probe and method for real-time monitoring of intracellular magnesium ions using the two-photon probe 有权
    用于实时监测细胞内镁离子的双光子探针，双光子探针的制备方法和使用双光子探针实时监测细胞内镁离子的方法

    公开(公告)号：US20080293088A1

    公开(公告)日：2008-11-27

    申请号：US12002027

    申请日：2007-12-14

    申请人： Bong-Rae Cho

    发明人： Bong-Rae Cho

    IPC分类号： C12Q1/20 ,  C07C275/00

    CPC分类号： C07C237/04

    摘要： A two-photon probe for real-time monitoring of intracellular magnesium ions is provided. Specifically, the two-photon probe is represented by Formula 1: wherein R is H or CH2OCOCH3. The two-photon probe is very suitable for real-time imaging of intracellular magnesium ions. The two-photon probe shows 17-fold two-photon excited fluorescence enhancement in response to Mg2+, which is 7-fold stronger than commercial probes, thus enabling staining of cells in a greatly reduced amount. In addition, the two-photon probe has a sufficiently low molecular weight to stain cells and is very suitable for monitoring Mg2+ ions present in the deep tissue. Furthermore, the two-photon probe can be effectively used for the quantitative as well as qualitative detection of intracellular magnesium ions. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular magnesium ions using the two-photon probe.

    摘要翻译： 提供了用于实时监测细胞内镁离子的双光子探针。 具体地，双光子探针由式1表示：其中R是H或CH 2OCOCH 3。 双光子探针非常适合细胞内镁离子的实时成像。 双光子探针显示出响应于Mg2 +的17倍双光子激发荧光增强，其比商业探针强7倍，从而能够大大减少细胞的染色。 此外，双光子探针具有足够低的分子量以染色细胞，并且非常适合于监测存在于深层组织中的Mg 2+离子。 此外，双光子探针可以有效地用于定量和定性检测细胞内镁离子。 还提供了制备双光子探针的方法和使用双光子探针实时监测细胞内镁离子的方法。

    Synthetic human peptides and pharmaceutical compositions comprising them for the treatment of systemic lupus erythematosus
    120.
    发明申请
    Synthetic human peptides and pharmaceutical compositions comprising them for the treatment of systemic lupus erythematosus 有权
    包含它们的合成人肽和药物组合物用于治疗系统性红斑狼疮

    公开(公告)号：US20080119390A1

    公开(公告)日：2008-05-22

    申请号：US11894472

    申请日：2007-08-20

    申请人： Edna Mozes

    发明人： Edna Mozes

    IPC分类号： A61K38/00 ,  C07K16/00 ,  C12Q1/20 ,  A61P43/00

    CPC分类号： C07K16/18 ,  A61K39/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/73

    摘要： Synthetic peptides of at least 12 and at most 30 amino acid residues comprising a sequence consisting of, or found within, a complementarity-determining region (CDR) found in the heavy or light chain of the human anti-DNA 16/6Id monoclonal antibody, or a sequence obtained by replacement and/or deletion and/or addition of one or more amino residues to said sequence, and salts, chemical derivatives and polymers of said peptides can be used for immunomodulation of systemic lupus erythematosus-associated responses.

    摘要翻译： 包含由人抗-DNA 16 / 6Id单克隆抗体的重链或轻链中发现的互补决定区（CDR）组成或发现的序列的至少12个和至多30个氨基酸残基的合成肽， 或通过将所述序列替换和/或缺失和/或添加一个或多个氨基残基获得的序列，以及所述肽的盐，化学衍生物和聚合物可用于系统性红斑狼疮相关应答的免疫调节。