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201 条记录 (耗时 0.044 秒)

    Methods for analyzing nucleic acid sequences
    132.
    发明授权
    Methods for analyzing nucleic acid sequences 有权
    分析核酸序列的方法

    公开(公告)号：US07943307B2

    公开(公告)日：2011-05-17

    申请号：US11336122

    申请日：2006-01-19

    申请人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    发明人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/00 ,  C12M3/00 ,  C07H21/04 ,  G01N33/53

    CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/537 ,  C12Q2537/149 ,  C12Q2561/113 ,  C12Q2521/543 ,  C12Q2565/518 ,  C12Q2561/12

    摘要： The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.

    摘要翻译： 本发明涉及对靶核酸进行测序的方法。 该方法提供了一种包含聚合酶，靶核酸分子和引物的复合物，其中复合物被固定在载体上荧光标记附着于核苷酸或核苷酸类似物的末端磷酸基团。 通过使用聚合酶将核酸类似物加入到核酸链中来延长生长的核酸链。 鉴定作为聚合步骤的结果添加到寡核苷酸引物中的核苷酸类似物。 来自被引入的标记核苷酸或核苷酸类似物的信号的持续时间与自由扩散标记的区别在于，对于被掺入的核苷酸或核苷酸类似物的观察体积比对于自由扩散标记物的观察体积更长的保留。

    Method of forming a tablet
    135.
    发明授权
    Method of forming a tablet 失效
    形成片剂的方法

    公开(公告)号：US07749537B2

    公开(公告)日：2010-07-06

    申请号：US11906303

    申请日：2007-10-01

    申请人： Michael Hite ,  Cathy Federici ,  Alan Brunelle ,  Stephen Turner

    发明人： Michael Hite ,  Cathy Federici ,  Alan Brunelle ,  Stephen Turner

    IPC分类号： A61K9/20 ,  A61K31/192

    CPC分类号： A61K9/2013 ,  A61K9/2031 ,  A61K9/2054 ,  A61K31/192

    摘要： A method of forming a tablet includes the steps of pre-blending an active pharmaceutical ingredient susceptible to tackiness and a blending additive with a first mixing effort to form a pre-blend mixture, wherein the first mixing effort and a second mixing effort, resulting from mixing at least one excipient with the pre-blend mixture, form a blend suitable for direct compression and compressing the blend to form the tablet. One way of achieving the first mixing effort is to pre-blend for an extended period of time. The method allows for directly compressing the blend without the need for a granulation step or roller compression. One such active pharmaceutical ingredient susceptible to tackiness is ibuprofen.

    摘要翻译： 形成片剂的方法包括以下步骤：将具有粘性的活性药物成分和混合添加剂预先混合以形成预混物混合物，其中第一混合力和第二混合力由 将至少一种赋形剂与预混物混合物混合，形成适于直接压缩和压缩混合物以形成片剂的共混物。 实现第一次混合努力的一种方式是预混合一段较长的时间。 该方法允许直接压缩共混物，而不需要造粒步骤或辊压缩。 易于粘性的一种这样的活性药物成分是布洛芬。

    Methods and systems for analyzing fluorescent materials with reduced authofluorescence
    136.
    发明授权
    Methods and systems for analyzing fluorescent materials with reduced authofluorescence 有权
    用荧光物质分析荧光降低的方法和系统

    公开(公告)号：US07714303B2

    公开(公告)日：2010-05-11

    申请号：US12151979

    申请日：2008-05-09

    申请人： Paul Lundquist ,  Cheng Frank Zhong ,  Denis Zaccarin ,  Stephen Turner

    发明人： Paul Lundquist ,  Cheng Frank Zhong ,  Denis Zaccarin ,  Stephen Turner

    IPC分类号： F21V9/16

    CPC分类号： G01N27/447

    摘要： Mitigative and remedial approaches to reduction of autofluorescence background noise are applied in analytical systems that rely upon sensitive measurement of fluorescent signals from arrays of fluorescent signal sources. Such systems are for particular use in fluorescence based sequencing by incorporation systems that rely upon small numbers or individual fluorescent molecules in detecting incorporation of nucleotides in primer extension reactions.

    摘要翻译： 降低自发荧光背景噪声的缓解和补救方法应用于依赖于荧光信号源阵列的荧光信号敏感测量的分析系统中。 这样的系统特别用于基于荧光的测序，其通过依赖于小数目或单独的荧光分子检测引物延伸反应中核苷酸的掺入的并入系统。

    Composition for nucleic acid sequencing
    139.
    发明授权
    Composition for nucleic acid sequencing 有权
    用于核酸测序的组合物

    公开(公告)号：US07416844B2

    公开(公告)日：2008-08-26

    申请号：US11285422

    申请日：2005-11-21

    申请人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    发明人： Jonas Korlach ,  Watt W. Webb ,  Michael Levene ,  Stephen Turner ,  Harold G. Craighead ,  Mathieu Foquet

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12P21/06 ,  C12M1/34 ,  C12N11/00 ,  G01N33/00 ,  G01N33/53 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/537 ,  C12Q2537/149 ,  C12Q2561/113 ,  C12Q2521/543 ,  C12Q2565/518 ,  C12Q2561/12

    摘要： The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

    摘要翻译： 本发明涉及对具有多个碱基的靶核酸分子进行测序的方法。 在其原理中，聚合反应中碱添加的时间顺序是在核酸分子上测量的，即核酸聚合酶在待测序的模板核酸分子上的活性被实时跟踪。 通过在碱添加序列的每个步骤中通过核酸聚合酶的催化活性鉴定哪个碱基被掺入靶核酸的生长互补链中来推断该序列。 在靶核酸分子复合物上提供聚合酶，其适于沿着靶核酸分子移动并在活性位点延伸寡核苷酸引物。 多个标记类型的核苷酸类似物在活性位点附近提供，每种可区分类型的核苷酸类似物与靶核酸序列中的不同核苷酸互补。 生长的核酸链通过使用聚合酶延伸到活性位点处的核酸链的核苷酸类似物，其中加入的核苷酸类似物与活性位点上的靶核酸的核苷酸互补。 鉴定作为聚合步骤的结果添加到寡核苷酸引物中的核苷酸类似物。 重复提供标记的核苷酸类似物，聚合生长的核酸链和鉴定添加的核苷酸类似物的步骤，使得核酸链进一步延长并确定靶核酸的序列。