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143 条记录 (耗时 0.027 秒)

    Method of cell culture
    131.
    发明授权
    Method of cell culture 有权
    细胞培养方法

    公开(公告)号：US08759100B2

    公开(公告)日：2014-06-24

    申请号：US13990642

    申请日：2010-12-06

    申请人： Naoki Yokoyama ,  Tomonori Akai

    发明人： Naoki Yokoyama ,  Tomonori Akai

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0068 ,  C12M25/08 ,  C12M33/00 ,  C12N2529/00 ,  C12N2539/10

    摘要： This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated.

    摘要翻译： 本发明提供了在特定条件下修饰细胞培养基质的表面特性的方法，根据细胞种类，调节允许细胞粘附或不允许粘附的区域。 本发明涉及一种细胞培养方法，其包括以下步骤：借助于硅烷将正电位施加到包括具有导电区域的基底材料和与其偶联的非细胞粘附膜的基底的导电区域，以便 以将非细胞粘附膜与基底分离; 并在非细胞粘附膜分离的区域中培养细胞。

    Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus
    132.
    发明申请
    Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus 审中-公开
    交替离子磁共振（AIMR）多重培养培养装置

    公开(公告)号：US20130267020A1

    公开(公告)日：2013-10-10

    申请号：US13859180

    申请日：2013-04-09

    申请人： Thomas J. Goodwin ,  Moses J. Kushman

    发明人： Thomas J. Goodwin ,  Moses J. Kushman

    IPC分类号： C12N13/00

    CPC分类号： C12N13/00 ,  C12M23/24 ,  C12M23/28 ,  C12M27/10 ,  C12M27/20 ,  C12M29/04 ,  C12M35/02 ,  C12M35/04 ,  C12M35/06 ,  C12N5/0062 ,  C12N2513/00 ,  C12N2529/00

    摘要： Provided herein are a culture apparatus, culture systems and an alternating ionic magnetic resonance electromagnetic chamber for culturing cells, tissues or organoid bodies or for delivering a pulsating alternating ionic magnetic resonance field to an object of interest including the above or to an animal, human or plant. The culture apparatus comprises a culture unit having growth and nutrient modules and a randomizing adapter to continually randomize the gravity vector in the growth module. The culture systems further comprise the alternating ionic magnetic resonance electromagnetic chamber.

    摘要翻译： 本文提供了培养装置，培养系统和用于培养细胞，组织或组织体的交替离子磁共振电磁室，或用于将脉动交替离子磁共振场输送到包括上述目标的物体或动物，人类或 厂。 培养装置包括具有生长和营养模块的培养单元和随机化适配器，以使生长模块中的重力矢量不断地随机化。 培养系统还包括交替离子磁共振电磁室。

    Application of electrical stimulation for functional tissue engineering in vitro and in vivo
    137.
    发明授权
    Application of electrical stimulation for functional tissue engineering in vitro and in vivo 有权
    电刺激在体外和体内功能组织工程中的应用

    公开(公告)号：US08367410B2

    公开(公告)日：2013-02-05

    申请号：US10872577

    申请日：2004-06-21

    申请人： Milica Radisic ,  Hyoungshin Park ,  Robert Langer ,  Lisa Freed ,  Gordana Vunjak-Novakovic

    发明人： Milica Radisic ,  Hyoungshin Park ,  Robert Langer ,  Lisa Freed ,  Gordana Vunjak-Novakovic

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/07

    CPC分类号： C12N5/0658 ,  C12N2502/02 ,  C12N2502/1358 ,  C12N2529/00

    摘要： The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

    摘要翻译： 本发明提供了体外制备生物人造组织当量的新方法及其在体内植入后的增强整合。 这些方法包括在体外培养过程中将组织构建物递送至仿生电刺激，以改善其结构和功能性质，和/或在植入该构建体后体内，以增强其与宿主组织的整合并增加细胞存活和功能。 本发明的方法特别可用于生产生物人造等同物和/或修复和替换含有电可兴奋细胞的天然组织，并且在体内受到电刺激，例如心肌组织，条纹骨骼肌组织 ，平滑肌肉组织，骨骼，脉管系统和神经组织。

    METHOD FOR PREPARING A SUBSTRATE FOR ARRANGING ANIMAL CELLS IN AN ARRAY AND METHOD FOR PREPARING A SUBSTRATE ON WHICH ANIMAL CELLS ARE ARRANGED IN AN ARRAY
    138.
    发明申请
    METHOD FOR PREPARING A SUBSTRATE FOR ARRANGING ANIMAL CELLS IN AN ARRAY AND METHOD FOR PREPARING A SUBSTRATE ON WHICH ANIMAL CELLS ARE ARRANGED IN AN ARRAY 审中-公开
    用于制备用于在基因组中安排动物细胞的基质的方法以及用于制备动物细胞在阵列中安置的基底的方法

    公开(公告)号：US20120270755A1

    公开(公告)日：2012-10-25

    申请号：US13504509

    申请日：2010-10-29

    申请人： Sumihiro Koyama

    发明人： Sumihiro Koyama

    IPC分类号： C40B50/06

    CPC分类号： C12N5/0068 ,  C12N2529/00 ,  C12N2533/90

    摘要： A new means of separately arranging individual animal cells on a substrate surface is provided. A method for preparing a substrate for arranging animal cells in an array, comprising steps (1) to (3): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; and (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with the extracellular matrix adhered to the adsorption surfaces thereof. A method for preparing a substrate on which animal cells have been arranged in an array, comprising steps (1) to (4): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with extracellular matrix adhered to the adsorption surfaces thereof; and (4) culturing the animal cells on the surface of the substrate obtained in (3) to obtain a substrate on which the animal cells have adhered to the adsorption surface.

    摘要翻译： 提供了一种在基片表面上分别布置单个动物细胞的新方法。 一种制备用于将阵列中的动物细胞排列的衬底的方法，包括步骤（1）至（3）：（1）在电极衬底表面上制备阵列中具有吸附表面的衬底; （2）使细胞外基质吸附到基板的电极表面和吸附面上; （3）向电极施加弱电位使电极表面上的细胞外基质分离，得到附着于其吸附表面的细胞外基质的底物。 一种制备其上已经排列有动物细胞的基板的方法，包括步骤（1）至（4）：（1）在电极基板表面上制备阵列中具有吸附表面的基板; （2）使细胞外基质吸附到基板的电极表面和吸附面上; （3）向电极施加弱电位使电极表面上的细胞外基质分离，得到附着于其吸附表面的细胞外基质的底物; （4）在（3）得到的基材的表面上培养动物细胞，得到动物细胞附着于吸附面的基材。

    Method and apparatus for changing one type of cell into another type of cell
    140.
    发明申请
    Method and apparatus for changing one type of cell into another type of cell 审中-公开
    将一种类型的细胞改变成另一种类型的细胞的方法和装置

    公开(公告)号：US20120129224A1

    公开(公告)日：2012-05-24

    申请号：US13288801

    申请日：2011-11-03

    申请人： Chauncey B. Sayre

    发明人： Chauncey B. Sayre

    IPC分类号： C12P19/34 ,  C12M1/42 ,  C07K1/14 ,  C12N15/85 ,  C12N13/00

    CPC分类号： C12M35/02 ,  C12N5/00 ,  C12N2506/00 ,  C12N2529/00

    摘要： A method and apparatus converts host cells of a first type into cells of a second type when the host cells are placed in intimate contact with donor cells of the second type. Under predetermined conditions there is transport of a sufficient number of mRNA molecules from the donor cells into the host cells to reprogram the host cells into the second type. The host and donor cells may be subjected to while in intimate contact to a transporting force that enables the mRNA molecules of the donor cells to penetrate an outer membrane wall of host cells without damaging the membrane wall. The transporting force may include an electric field, a magnetic field, or a combined electric field and magnetic field.

    摘要翻译： 当宿主细胞与第二类型的供体细胞紧密接触时，方法和装置将第一类型的宿主细胞转化为第二类型的细胞。 在预定条件下，将足够数量的mRNA分子从供体细胞转运到宿主细胞中以将宿主细胞重编程为第二类型。 宿主和供体细胞可以经受紧密接触以使得供体细胞的mRNA分子能够渗透宿主细胞的外膜壁而不破坏膜壁的传递力。 输送力可以包括电场，磁场或组合的电场和磁场。