公开(公告)号：US09694361B2

公开(公告)日：2017-07-04

申请号：US14682952

申请日：2015-04-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  G01N15/06 ,  C12M3/00 ,  B01F3/00 ,  B01F3/04 ,  B01L3/02 ,  G01N35/08 ,  G01N35/00 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N35/085 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US09644204B2

公开(公告)日：2017-05-09

申请号：US14175935

申请日：2014-02-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34 ,  G01N33/50 ,  C12N15/10 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20160281137A1

公开(公告)日：2016-09-29

申请号：US15174923

申请日：2016-06-06

Applicant: 10X Genomics, Inc.

Inventor： Mirna Jarosz ,  Michael Schnall-Levin ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/514 ,  C12Q2563/179

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20160281136A1

公开(公告)日：2016-09-29

申请号：US15174919

申请日：2016-06-06

Applicant: 10X Genomics, Inc.

Inventor： Mirna Jarosz ,  Michael Schnall-Levin ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/514 ,  C12Q2563/179

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

Abstract translation: 本发明涉及用于捕获和分析包含在基因组目标区域中的序列信息的方法，组合物和系统。 这些靶向区域可以包括外显子，部分外显子，内含子，外显子和内含子区域的组合，基因，基因组，以及可能感兴趣的整个基因组的任何其它子集。

公开(公告)号：US09388465B2

公开(公告)日：2016-07-12

申请号：US14175973

申请日：2014-02-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12N15/10 ,  C12Q1/68 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

Abstract translation: 本公开提供了用于多核苷酸处理的组合物，方法，系统和装置。 这种多核苷酸处理可用于多种应用，包括多核苷酸测序。 在一些情况下，本公开提供了产生多核苷酸条形码文库以及将这些多核苷酸连接到靶多核苷酸的方法。

公开(公告)号：US20150376609A1

公开(公告)日：2015-12-31

申请号：US14752641

申请日：2015-06-26

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Grace Zheng ,  Phillip Belgrader

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C40B20/04 ,  C40B50/16

Abstract: Methods, compositions and systems for analyzing individual cells or cell populations through the partitioned analysis of contents of individual cells or cell populations. Individual cells or cell populations are co-partitioned with processing reagents for accessing cellular contents, and for uniquely identifying the contents of a given cell or cell population, and subsequently analyzing the cell's contents and characterizing it as having derived from an individual cell or cell population, including analysis and characterization of the cell's nucleic acids through sequencing.

Abstract translation: 通过对单个细胞或细胞群体的内容进行分区分析来分析个体细胞或细胞群体的方法，组合物和系统。 将个体细胞或细胞群与用于获取细胞内容物的处理试剂共同分配，并且用于唯一地鉴定给定细胞或细胞群体的内容，随后分析细胞的内容并将其表征为衍生自单个细胞或细胞群体 ，包括通过测序分析和表征细胞的核酸。

公开(公告)号：US20150225777A1

公开(公告)日：2015-08-13

申请号：US14624468

申请日：2015-02-17

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01J19/0046 ,  B01L3/502715 ,  B01L3/508 ,  B01L3/523 ,  B01L2200/0647 ,  B01L2400/0677 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.

Abstract translation: 本公开提供了微孔封装阵列器件。 微孔胶囊阵列装置通常能够执行一个或多个样品制备操作。 这种样品制备操作可以用作一个或多个分析操作的前奏。 例如，本公开的装置可以在单个单元内实现具有独特分子标识符的样品的物理分配和离散混合，以准备各种分析操作。 该装置可用于各种应用，最值得注意的是基于核酸的测序，基因表达和单细胞分析的检测和定量。