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88 条记录 (耗时 0.025 秒)

    Amplification of nucleic acids
    11.
    发明申请
    Amplification of nucleic acids 审中-公开
    核酸扩增

    公开(公告)号：US20050136417A1

    公开(公告)日：2005-06-23

    申请号：US10796323

    申请日：2004-03-09

    申请人： Kyle Cole ,  Vivi Truong ,  Glenn McGall

    发明人： Kyle Cole ,  Vivi Truong ,  Glenn McGall

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2600/156 ,  C12Q2531/119 ,  C12Q2525/119 ,  C12Q2521/301

    摘要： Methods and kits for amplifying nucleic acids are provided. Double stranded cDNA with uridine residues incorporated into one strand is synthesized and the uridine containing strand is nicked at uridine residues. The DNA is extended from the nicks in the presence of dUTP using a strand displacing enzyme. Repeated cycles of nicking and extension result in amplification of the nucleic acid. Methods are also provided for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for example, the presence or absence of one or more polymorphisms or the presence or absence of a transcript.

    摘要翻译： 提供了用于扩增核酸的方法和试剂盒。 合成具有并入一条链中的尿苷残基的双链cDNA，并将含有尿苷的链在尿苷残基上切口。 使用链置换酶，在dUTP存在下，DNA从缺口延伸。 切口和延伸的重复循环导致核酸的扩增。 还提供了用于通过与阵列杂交来分析上述样品的方法，该阵列可被特别设计用于询问特定特征的靶序列的收集，例如存在或不存在一个或多个多态性或存在 或没有抄本。

    Phosphite ester oxidation in nucleic acid array preparation
    12.
    发明授权
    Phosphite ester oxidation in nucleic acid array preparation 有权
    亚磷酸酯氧化在核酸阵列制备中

    公开(公告)号：US06833450B1

    公开(公告)日：2004-12-21

    申请号：US09810434

    申请日：2001-03-15

    申请人： Glenn McGall ,  Lisa Kajisa

    发明人： Glenn McGall ,  Lisa Kajisa

    IPC分类号： C07H2100

    CPC分类号： C07H21/00 ,  Y10S436/809

    摘要： Methods are provided for preparing nucleic acid arrays on a support. In these methods a plurality of nucleic acids are synthesized on the support and the synthesis steps include oxidizing a phosphite triester nucleic acid linkage to a phosphate triester nucleic acid linkage using a solution about 0.005 to about 0.05 M iodine in a mixture comprising water and organic solvent.

    摘要翻译： 提供了在载体上制备核酸阵列的方法。 在这些方法中，在载体上合成多个核酸，合成步骤包括使用约0.005至约0.05M碘的溶液在含水和有机溶剂的混合物中将亚磷酸三酯核酸键氧化成磷酸三酯核酸连接 。

    Functionalized silicon compounds and methods for their synthesis and use
    13.
    发明授权
    Functionalized silicon compounds and methods for their synthesis and use 有权

    公开(公告)号：US06429275B2

    公开(公告)日：2002-08-06

    申请号：US09832656

    申请日：2001-04-11

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G7700

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

    Nucleic Acid Labeling Compounds
    19.
    发明申请
    Nucleic Acid Labeling Compounds 有权
    核酸标记化合物

    公开(公告)号：US20080287667A1

    公开(公告)日：2008-11-20

    申请号：US12163528

    申请日：2008-06-27

    申请人： Glenn McGall ,  Anthony D. Barone

    发明人： Glenn McGall ,  Anthony D. Barone

    IPC分类号： C07H21/00 ,  C07H19/04 ,  C07D413/04

    CPC分类号： C12Q1/6816 ,  C07D239/47 ,  C07H7/00 ,  C07H7/06 ,  C07H19/06

    摘要： Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofaranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

    摘要翻译： 公开了核酸标记化合物。 该化合物是通过将杂环衍生物与环状基团（例如核糖霜糖衍生物）缩合而合成的。 标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

    Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
    20.
    发明申请
    Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog 审中-公开
    使用核糖核苷三磷酸酯类似物进行cDNA的标记和非酶切片段化

    公开(公告)号：US20070154894A1

    公开(公告)日：2007-07-05

    申请号：US11323068

    申请日：2005-12-30

    申请人： Glenn McGall ,  Handong Li ,  Anthony Barone

    发明人： Glenn McGall ,  Handong Li ,  Anthony Barone

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12N15/1096 ,  C12Q1/6806 ,  C12Q2523/107

    摘要： In accordance with the present invention, methods are presented for labeling a cDNA strand with a labeled ribonucleotide base precursor which upon exposure to Mg2+, heat and base cleaves the cDNA at each place of incorporation of an RNA. In accordance with an aspect of the present invention, compounds selected from the group consisting of are incorporated into the growing strand of a cDNA by a reverse transcriptase or a mutant reverse transcriptase. After subject the strands to Mg2+, base and heat, the 3′ OH causes cleavage of the cDNA leaving a 2′OH phosphate with a biotin label. The biotin provides a label which may be bound to streptavidin and thereafter hybridized to a nucleic acid array.

    摘要翻译： 根据本发明，提供了用标记的核糖核苷酸碱基前体标记cDNA链的方法，其在暴露于Mg 2+时，加热和碱基在每个引入RNA的位置切割cDNA。 根据本发明的一个方面，选自组合的化合物通过逆转录酶或突变逆转录酶掺入cDNA的生长链中。 在将基因经过碱基和加热后，3'OH引起用生物素标记物离开2'OH磷酸酯的cDNA的切割。 生物素提供可以与链霉抗生物素蛋白结合的标记，然后与核酸阵列杂交。