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公开(公告)号：US20050208646A1
公开(公告)日：2005-09-22
申请号：US10995882
申请日：2004-11-22
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12M1/34
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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公开(公告)号：US20050106618A1
公开(公告)日：2005-05-19
申请号：US11015197
申请日：2004-12-16
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12Q1/68
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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公开(公告)号：US20060234267A1
公开(公告)日：2006-10-19
申请号：US11378954
申请日：2006-03-20
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
Abstract translation: 具有空腔310的主体300，其具有根据美国专利No. 提供了第5,143,854号和PCT WO 92/10092等。 空腔包括用于将选定流体引入空腔中以接触探针的入口350和360。 因此，提供了用于高通量测定系统的商业上可行的装置。
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公开(公告)号：US20050106617A1
公开(公告)日：2005-05-19
申请号：US10996201
申请日：2004-11-22
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12Q1/68
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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公开(公告)号：US20050003421A1
公开(公告)日：2005-01-06
申请号：US10877666
申请日：2004-06-25
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12Q1/68 , G01N33/48 , G01N33/50 , G01N33/53 , G06F19/00
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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Patent Agency Ranking
公开(公告)号：US20050191630A1
公开(公告)日：2005-09-01
申请号：US10639696
申请日：2003-08-11
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12Q1/68 , C12M1/34
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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公开(公告)号：US20050106615A1
公开(公告)日：2005-05-19
申请号：US10992043
申请日：2004-11-17
Applicant: Donald Besemer , Virginia Goss , James Winkler
Inventor： Donald Besemer , Virginia Goss , James Winkler
IPC: B01F13/00 , B01F13/02 , B01F13/08 , B01J19/00 , B01L3/00 , B01L9/00 , B81B1/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00 , H01L21/00 , C12Q1/68
CPC classification number: B01L3/502715 , B01F13/0054 , B01F13/0059 , B01F13/02 , B01F13/08 , B01F13/0818 , B01J19/0046 , B01J2219/00286 , B01J2219/00353 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0825 , B01L2300/0877 , B01L2300/1805 , B01L2400/0439 , B01L2400/0442 , B01L2400/0487 , B01L2400/0611 , B01L2400/065 , B82Y30/00 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N2035/00158 , H01L21/67144 , Y10T436/25
Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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公开(公告)号：US06239273B1
公开(公告)日：2001-05-29
申请号：US09427850
申请日：1999-10-26
Applicant: R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler
Inventor： R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler
IPC: C07M2100
CPC classification number: G03F7/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00382 , B01J2219/00385 , B01J2219/00427 , B01J2219/00432 , B01J2219/00454 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B41M3/00 , B82Y30/00 , C07B2200/11 , C07H21/00 , C07K1/047 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14
Abstract: A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.
Abstract translation: 一种用于选择性地将印刷材料施加到基底上以在基底的选定区域合成寡核苷酸阵列的方法和装置。 印刷材料包括阻挡材料，单体序列，核苷，去保护剂，载体材料等。 该方法和装置还依赖于基于标准的基于DMT的化学，以及气相脱保护剂如固体TCA等。
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9.发明授权Printing oligonucleotide arrays using deprotection agents solely in the vapor phase 失效使用去保护剂仅在气相中印刷寡核苷酸阵列
公开(公告)号：US5831070A
公开(公告)日：1998-11-03
申请号：US635272
申请日：1996-04-19
Applicant: R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler
Inventor： R. Fabian Pease , Glenn McGall , Martin J. Goldberg , Richard P. Rava , Stephen P. A. Fodor , Virginia Goss , Lubert Stryer , James L. Winkler
IPC: B01J19/00 , B41M3/00 , C07H21/00 , C40B40/06 , C40B50/14 , C40B60/14 , G03F7/00 , C07H21/02 , C07K5/00 , C07K16/00
CPC classification number: C07H21/00 , B01J19/0046 , G03F7/00 , B01J2219/00378 , B01J2219/00382 , B01J2219/00385 , B01J2219/00427 , B01J2219/00454 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00722 , B41M3/00 , C40B40/06 , C40B50/14 , C40B60/14
Abstract: A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.
Abstract translation: 一种用于选择性地将印刷材料施加到基底上以在基底的选定区域合成寡核苷酸阵列的方法和装置。 印刷材料包括阻挡材料，单体序列，核苷，去保护剂，载体材料等。 该方法和装置还依赖于基于标准的基于DMT的化学和气相脱保护剂如固体TCA等。
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公开(公告)号：US07363165B2
公开(公告)日：2008-04-22
申请号：US09811762
申请日：2001-03-19
Applicant: Virginia Goss Tusher , Robert Tibshirani , Gilbert Chu
Inventor： Virginia Goss Tusher , Robert Tibshirani , Gilbert Chu
CPC classification number: G06F19/20 , C12Q2600/158
Abstract: Microarrays can measure the expression of thousands of genes and thus identify changes in expression between different biological states. Methods are needed to determine the significance of these changes, while accounting for the enormous number of genes. We describe a new method, Significance Analysis of Microarrays (SAM), that assigns a score to each gene based on the change in gene expression relative to the standard deviation of repeated measurements. For genes with scores greater than an adjustable threshold, SAM uses permutations of the repeated measurements to estimate the percentage of such genes identified by chance, the false discovery rate (FDR). When the transcriptional response of human cells to ionizing radiation was measured by microarrays, SAM identified 34 genes that changed at least 1.5-fold with an estimated FDR of 12%, compared to FDRs of 60% and 84% using conventional methods of analysis. Of the 34 genes, 19 were involved in cell cycle regulation, and 3 in apoptosis. Surprisingly, 4 nucleotide excision repair genes were induced, suggesting that this repair pathway for UV-damaged DNA might play a heretofore unrecognized role in repairing DNA damaged by ionizing radiation.
Abstract translation: 微阵列可以测量数千个基因的表达，从而识别不同生物状态之间的表达变化。 需要方法来确定这些变化的重要性，同时占据了大量的基因。 我们描述了一种基于相对于重复测量的标准偏差的基因表达变化为每个基因分配分数的微阵列（SAM）的新方法。 对于分数大于可调阈值的基因，SAM使用重复测量的排列来估计偶然识别的这些基因的百分比，即错误发现率（FDR）。 当通过微阵列测量人类细胞对电离辐射的转录反应时，SAM使用常规分析方法鉴定了34个基因，其变化至少1.5倍，估计FDR为12％，而FDR为60％和84％。 在34个基因中，19个参与细胞周期调控，3个在凋亡中。 令人惊讶的是，诱导了4个核苷酸切除修复基因，表明这种UV损伤的DNA的修复途径可能在修复被电离辐射损伤的DNA中未被认识到的作用。
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