公开(公告)号：US20160186166A1

公开(公告)日：2016-06-30

申请号：US14964060

申请日：2015-12-09

Applicant: Life Technologies Corporation ,  Thermo Fisher Scientific Geneart Gmbh ,  Life Technologies As

Inventor： Thomas POEHMERER ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02 ,  B01L3/00 ,  C25B3/10

CPC classification number: C12N15/101 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C5/026 ,  B03C2201/26 ,  C25B3/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20160097094A1

公开(公告)日：2016-04-07

申请号：US14970192

申请日：2015-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofslokken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem TRAN ,  Jo AASERUD ,  Talha M. GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20140179025A1

公开(公告)日：2014-06-26

申请号：US14190054

申请日：2014-02-25

Applicant: LIFE TECHNOLOGIES AS

Inventor： Ellen Weng ,  Geir Fonnum ,  Grete Modahl ,  Astrid Molteberg ,  Erling Finne

IPC: G01N33/543

CPC classification number: C09K11/06 ,  B82Y25/00 ,  B82Y40/00 ,  C08F220/18 ,  C09K11/025 ,  G01N33/5434 ,  H01F1/0018 ,  H01F1/01 ,  H01F1/26 ,  H01F41/00 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: A coated magnetic particle comprising an optionally porous magnetic polymer particle of a matrix polymer, said polymer particle having on a surface and/or in the pores thereof superparamagnetic crystals, said coated particle having a coat formed of a coating polymer, wherein said coated magnetic particle is essentially non-autofluorescent.

公开(公告)号：US20140080966A1

公开(公告)日：2014-03-20

申请号：US14081263

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/10 ,  C08K3/20 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20130035418A1

公开(公告)日：2013-02-07

申请号：US13649045

申请日：2012-10-10

Applicant: Life Technologies AS

Inventor： Arne JORGEDAL ,  Elin Asknes ,  Geir Fonnum ,  Astrid Molteberg ,  Rolf Nordal ,  Henning Pettersen ,  Tollef Taarneby ,  Solveig Staale ,  Ellen Weng ,  Finn Hansen ,  Silje Nordbo ,  Oddvar Aune ,  Arvid Berge ,  John Bjorgum ,  Turid Ellingsen ,  John Ugelstad

IPC: C08L25/06 ,  C08K5/41 ,  C08K5/14 ,  C08K5/06 ,  C08L33/08 ,  C08L27/04

CPC classification number: C08J9/16 ,  C08F2/22 ,  C08F257/02 ,  C08F265/04 ,  C08F265/10 ,  C08F291/00 ,  C08J9/22 ,  C08J9/224 ,  C08J2203/14 ,  C08J2325/06 ,  C08J2347/00

Abstract: A process for the preparation of monodisperse polymer particles which are formed by contacting monomers with aqueous dispersions comprising monodisperse swellable seed polymers/oligomers, and initiating polymerization in the presence of a steric stabilizer. The resulting swollen seed particles are characterized by the particle mode diameter.

Abstract translation: 通过使单体与包含单分散可溶胀种子聚合物/低聚物的水性分散体接触而形成的单分散聚合物颗粒的制备方法，以及在空间稳定剂存在下引发聚合。 所得的溶胀种子颗粒的特征在于颗粒模式直径。

公开(公告)号：US20230272127A1

公开(公告)日：2023-08-31

申请号：US18143880

申请日：2023-05-05

Applicant: Life Technologies AS

Inventor： Per Nils Pontus Lundberg ,  Geir Fonnum ,  Kristian Andersson

IPC: C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08L33/26

CPC classification number: C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08L33/26 ,  C08K3/22

Abstract: This invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.6; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse coated hydrogel polymer particles.

公开(公告)号：US11555183B2

公开(公告)日：2023-01-17

申请号：US16645896

申请日：2018-08-30

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure Jobert

IPC: C12N15/10

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

公开(公告)号：US20220162531A1

公开(公告)日：2022-05-26

申请号：US17671353

申请日：2022-02-14

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： Lars Norderhaug ,  Angel Varela-Rohena ,  Nephi D. Jones

IPC: C12M1/26 ,  B03C1/30 ,  B03C1/01 ,  B03C1/28 ,  A61M1/36 ,  B01L3/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12

Abstract: A magnetic particle separation system includes a magnetic field generating device having an upper surface with a receiving area formed thereon; and a magnetic field generating element disposed beneath the upper surface, the magnetic field generating element being configured to produce a magnetic field above the upper surface. A container assembly is disposed on the upper surface and includes: a flexible container having an outer wall with an interior surface that at least partially bounds an internal compartment, the outer wall having a front side and an opposing back side with the internal compartment disposed therebetween; a fluid inlet extending through the outer wall at the front side; a fluid outlet extending through the outer wall at the front side; and a first partition projecting into the internal compartment from the front side between the fluid inlet and the fluid outlet.

公开(公告)号：US20200010827A1

公开(公告)日：2020-01-09

申请号：US16518099

申请日：2019-07-22

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US10189956B2

公开(公告)日：2019-01-29

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir Fonnum ,  Steven M. Menchen ,  M. Talha Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08F8/12 ,  C08J3/075 ,  C08F220/58 ,  C08F220/70 ,  C08F230/08

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.