-
11.发明授权公开(公告)号：US08158390B2
公开(公告)日：2012-04-17
申请号：US11663984
申请日：2005-08-19
Applicant: Jan Marienhagen , Lothar Eggeling , Hermann Sahm
Inventor： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
CPC classification number: C12P13/08 , C12N9/1096
Abstract: The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacterium glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.
Abstract translation: 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。
-
公开(公告)号：US20110039313A1
公开(公告)日：2011-02-17
申请号：US12517923
申请日：2008-01-10
Applicant: Stefan Verseck , Harald Haeger , Andreas Karau , Lothar Eggeling , Hermann Sahm
Inventor： Stefan Verseck , Harald Haeger , Andreas Karau , Lothar Eggeling , Hermann Sahm
CPC classification number: C12N9/88 , C12P13/001
Abstract: The invention relates to recombinant microorganisms in which polynucleotides which code for lysine decarboxylase are enhanced and, using which, cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw materials such as, for example, glucose, sucrose, molasses and the like.
Abstract translation: 本发明涉及重组微生物，其中编码赖氨酸脱羧酶的多核苷酸被增强，并且使用其发酵生成尸胺（1,5-二氨基戊烷），所用的碳源优选是可再生原料，例如葡萄糖 ，蔗糖，糖蜜等。
-
公开(公告)号：US07632663B1
公开(公告)日：2009-12-15
申请号：US09914006
申请日：2000-02-21
Applicant: Lothar Eggeling , Hermann Sahm
Inventor： Lothar Eggeling , Hermann Sahm
CPC classification number: C12N9/93 , C12N9/1014 , C12N9/88 , C12P13/06
Abstract: The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.
Abstract translation: 本发明涉及微生物生产L-缬氨酸的方法，其中二羟基酸合酶（ilvD）活性和/或ilvD基因表达在微生物中增强。 作为替代或与其结合，在微生物中增强乙酰羟酸合酶（ilvBN）活性和异构还原酶（ilvC）活性和/或ilvBNC基因表达。 根据本发明的方法优选利用其中减少或消除了减少L-缬氨酸形成的参与代谢途径的至少一种酶的活性的微生物。 因此，例如，根据本发明的方法优选利用在苏氨酸脱水酶（ilvA）基因中具有缺陷突变的微生物和/或泛酸合成的一种或多种基因中的缺陷突变。
-
14.发明申请NUCLEOTIDE SEQUENCES OF CORYNEFORM BACTERIA CODED FOR PROTEINS PARTICIPATING IN L-SERINE METABOLISM AND METHOD FOR MICROBIAL PRODUCTION OF L-SERINE 失效编码用于蛋白质参与L-丝氨酸代谢的CORYNEFORM细菌的核苷酸序列和用于微生物生产L-丝氨酸的方法公开(公告)号：US20090061482A1
公开(公告)日：2009-03-05
申请号：US12136190
申请日：2008-06-10
Applicant: Petra Peters-Wendisch , Roman Netzer , Lothar Eggeling , Hermann Sahm
Inventor： Petra Peters-Wendisch , Roman Netzer , Lothar Eggeling , Hermann Sahm
Abstract: This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
Abstract translation: 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
-
15.发明申请Nucleotide sequences of coryneform bacteria coding for proteins involved in l-serine metabolism and method for producing l-serine 审中-公开编码参与l-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于产生l-丝氨酸的方法公开(公告)号：US20060204963A1
公开(公告)日：2006-09-14
申请号：US10549262
申请日：2004-02-12
Applicant: Petra Peters-Wendisch , Roman Netzer , Lothar Eggeling , Hermann Sahm
Inventor： Petra Peters-Wendisch , Roman Netzer , Lothar Eggeling , Hermann Sahm
Abstract: The invention relates to the nucleotide sequences of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase. Said invention also relates to micro-organisms and to methods for producing L-serine.
Abstract translation: 本发明涉及编码与减少和关闭L-丝氨酸脱水酶的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 所述发明还涉及微生物和生产L-丝氨酸的方法。
-
Patent Agency Ranking
公开(公告)号：US20060014259A9
公开(公告)日：2006-01-19
申请号：US10091342
申请日：2002-03-06
Applicant: Kevin Burke , Hermann Sahm , Lothar Eggeling , Bernd Moritz , L. Dunican , Ashling McCormack , Cliona Stapelton , Bettina Mockel , Georg Thierbach , Rita Dunican
Inventor： Kevin Burke , Hermann Sahm , Lothar Eggeling , Bernd Moritz , L. Dunican , Ashling McCormack , Cliona Stapelton , Bettina Mockel , Georg Thierbach , Rita Dunican
CPC classification number: C12N9/0006 , C12N15/77 , C12P13/08 , C12Y101/01049
Abstract: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
-
公开(公告)号：US06680186B2
公开(公告)日：2004-01-20
申请号：US09829931
申请日：2001-04-11
Applicant: Madhavan Nampoothiri , Brigitte Bathe , Lothar Eggeling , Hermann Sahm
Inventor： Madhavan Nampoothiri , Brigitte Bathe , Lothar Eggeling , Hermann Sahm
IPC: C12N910
CPC classification number: C12N9/1029 , C12P13/04 , C12R1/13 , C12R1/15
Abstract: The present invention relates to polynucleotides corresponding to the plsC gene and which encode 1-acyl-SN-glycerol-3-phosphate acyltransferase, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having 1-acyl-SN-glycerol-3-phosphate acyltransferase activity.
Abstract translation: 本发明涉及对应于plsC基因并且编码1-酰基-SN-甘油-3-磷酸酰基转移酶的多核苷酸，产生L-氨基酸的方法以及筛选编码具有1-酰基-SN的蛋白质的多核苷酸的方法 甘油-3-磷酸酰基转移酶活性。
-
公开(公告)号：US06638753B2
公开(公告)日：2003-10-28
申请号：US09845248
申请日：2001-05-01
Applicant: Madhavan Nampoothiri K. , Bettina Möckel , Lothar Eggeling , Hermann Sahm
Inventor： Madhavan Nampoothiri K. , Bettina Möckel , Lothar Eggeling , Hermann Sahm
IPC: C12N910
CPC classification number: C12N9/1007 , C12P13/08 , C12P13/14
Abstract: The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.
-
19.发明授权L-lysine-producing corynebacteria and process for the preparation of L-lysine 有权L-赖氨酸生产棒杆菌和制备L-赖氨酸的方法公开(公告)号：US07981640B2
公开(公告)日：2011-07-19
申请号：US12249092
申请日：2008-10-10
Applicant: Caroline Kruetzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Sahm Hermann , Miroslav Patek
Inventor： Caroline Kruetzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Sahm Hermann , Miroslav Patek
IPC: C12P13/08
Abstract: L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and a process for the preparation of L-lysine.
Abstract translation: 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过度表达，以及L-赖氨酸的制备方法。
-
公开(公告)号：US20100291644A1
公开(公告)日：2010-11-18
申请号：US12602593
申请日：2008-05-30
Applicant: Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
Inventor： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
CPC classification number: C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
Abstract: The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
Abstract translation: 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。
-
-
-
-
-
-
-
-
-
Search Results
49
records
(took 0.02 seconds)
