公开(公告)号：US20180355120A1

公开(公告)日：2018-12-13

申请号：US15780477

申请日：2016-11-29

Applicant: Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

Inventor： Koichi Kotaka ,  Takaoki Ishii ,  Junichi Sugahara ,  Ryota Sato ,  Junichi Noba

IPC: C08J3/02 ,  C07K14/435 ,  C07K1/14

CPC classification number: C08J3/02 ,  C07K1/14 ,  C07K14/435 ,  C07K14/43586 ,  C08L89/00

Abstract: Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.

公开(公告)号：US10065997B2

公开(公告)日：2018-09-04

申请号：US14764463

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: C07K14/43 ,  A61K8/64 ,  A61K47/42 ,  C08J9/28 ,  C08L89/00 ,  A61K8/42 ,  A61K8/46 ,  A61Q19/00 ,  B01J20/24 ,  C11B9/00 ,  A61K9/19 ,  C07K14/435 ,  A61K8/02 ,  A61K8/98

CPC classification number: C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K8/987 ,  A61K9/19 ,  A61K47/42 ,  A61K2800/10 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  B01J20/24 ,  C08J9/28 ,  C08J2201/0484 ,  C08J2201/0544 ,  C08J2205/022 ,  C08J2205/028 ,  C08J2389/00 ,  C08L89/00 ,  C11B9/00

Abstract: A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

公开(公告)号：US20180080147A1

公开(公告)日：2018-03-22

申请号：US15564142

申请日：2016-04-04

Applicant: SPIBER INC. ,  KOJIMA INDUSTRIES CORPORATION

Inventor： Kana ISHIDA ,  Hironori YAMAMOTO ,  Hiroaki SUZUMURA ,  Kazuhide SEKIYAMA

IPC: D01F4/02 ,  C07K14/435 ,  D01F1/02

CPC classification number: D01F4/02 ,  C07K14/435 ,  C07K14/43518 ,  D01C3/00 ,  D01F1/02

Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent. The solution has a moisture content of less than 5 mass % based on 100 mass % of the solution. A method for producing a polar solvent solution of the present invention includes changing a moisture content of the solution to adjust the viscosity of the solution. Further, another method for producing a polar solvent solution includes reducing a moisture content of the solution to increase the viscosity of the solution. Thus, the present invention provides a polar solvent solution that enables stable spinning and casting without lowering its viscosity when used as dopes for spinning, film, etc., and methods for producing the same.

公开(公告)号：US20150361144A1

公开(公告)日：2015-12-17

申请号：US14764463

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki OSAWA ,  Keisuke MORITA

IPC: C07K14/435 ,  C11B9/00 ,  A61Q19/00 ,  B01J20/24 ,  A61K47/42 ,  A61K8/98

CPC classification number: C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K8/987 ,  A61K9/19 ,  A61K47/42 ,  A61K2800/10 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  B01J20/24 ,  C08J9/28 ,  C08J2201/0484 ,  C08J2201/0544 ,  C08J2205/022 ,  C08J2205/028 ,  C08J2389/00 ,  C08L89/00 ,  C11B9/00

Abstract: A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

Abstract translation: 本发明的多肽多孔体是衍生自蜘蛛丝蛋白的多肽的多孔体。 多肽包括水不溶性多肽。 多孔多孔体的表观密度为0.1g / cm 3以下。 制备多孔多孔体的方法包括：溶液制备步骤，其中将多肽溶解在至少一种选自DMSO，DMF的溶剂中，并用无机盐溶解，得到多肽的溶液; 在溶液制备工序中产生的溶液用水溶性溶剂代替以获得多肽凝胶的步骤; 以及其中多肽凝胶被干燥的步骤。 因此，本发明提供了具有优异的吸水性的多孔多孔体和适用于生物体的多孔多孔体及其制造方法。

公开(公告)号：US20150291673A1

公开(公告)日：2015-10-15

申请号：US14437704

申请日：2013-12-17

Applicant: SPIBER INC.

Inventor： Kaori Sekiyama ,  Mizuki Ishikawa ,  Shinya Murata

IPC: C07K14/435 ,  B29C39/02 ,  B29C71/02 ,  C08J5/18 ,  B29C55/02

CPC classification number: C07K14/43518 ,  B29C39/02 ,  B29C55/02 ,  B29C71/02 ,  B29K2089/00 ,  B29K2867/003 ,  B29K2995/002 ,  B29L2007/008 ,  C08J5/18 ,  C08J2389/00 ,  C08L89/00

Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

Abstract translation: 本发明的膜包含衍生自蜘蛛丝蛋白的多肽。 膜的分解温度为240〜260℃。该膜吸收波长为200〜300nm的紫外线，并且在400〜780nm的波长下具有85％以上的透光率。 该膜在可见光区域是透明和无色的。 本发明的膜的制造方法包括：将来自蜘蛛丝蛋白质的多肽溶解在二甲基亚砜溶剂中以制备涂料; 并将涂料浇铸在基材的表面上。 因此，本发明提供能够容易地形成并具有良好的拉伸性的蜘蛛丝蛋白质膜及其制造方法。

公开(公告)号：US09051453B2

公开(公告)日：2015-06-09

申请号：US14346893

申请日：2012-10-29

Applicant: SPIBER INC.

Inventor： Junichi Sugahara ,  Kazuhide Sekiyama ,  Ryota Sato ,  Kaori Sekiyama ,  Mizuki Ishikawa ,  Shinya Murata ,  Kazuko Otomo

IPC: C07K14/435 ,  B29D99/00 ,  C08K5/41 ,  C08K5/20 ,  D01D5/04 ,  D01F4/02 ,  C08K3/16

CPC classification number: C08K5/41 ,  B29D99/0078 ,  C07K14/43518 ,  C08K3/16 ,  C08K5/20 ,  D01D5/04 ,  D01F4/02 ,  D01F6/68 ,  Y10S530/858

Abstract: A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

Abstract translation: 本发明的多肽溶液是多肽溶液，其中衍生自天然蜘蛛丝蛋白的多肽溶解在溶剂中。 溶剂含有选自以下（i） - （iii）中的至少一种：（i）DMSO; （ii）具有无机盐的DMSO; 和（iii）具有无机盐的DMF。 此外，在本发明中，通过以下方式获得人造多肽纤维：使用多肽溶液作为掺杂溶液; 将喷丝头溶液从喷丝头挤出到去溶剂浴中，以从溶剂溶液中除去溶剂，并形成纤维以产生未拉伸的纱线。 此外，在本发明中，通过使多肽溶液进行热处理，然后从其中除去未溶解的物质来纯化多肽。 因此，本发明提供了溶质具有高溶解度且溶剂本身具有低成本且能够在高温下溶解并具有高安全性的多肽溶液。人造多肽纤维的制造方法以及纯化多肽的方法。

公开(公告)号：US20140245923A1

公开(公告)日：2014-09-04

申请号：US14346893

申请日：2012-10-29

Applicant: SPIBER INC.

Inventor： Junichi Sugahara ,  Kazuhide Sekiyama ,  Ryota Sato ,  Kaori Sekiyama ,  Mizuki Ishikawa ,  Shinya Murata ,  Kazuko Otomo

IPC: C08K5/41 ,  B29D99/00 ,  C08K3/16 ,  C07K14/435 ,  C08K5/16

CPC classification number: C08K5/41 ,  B29D99/0078 ,  C07K14/43518 ,  C08K3/16 ,  C08K5/20 ,  D01D5/04 ,  D01F4/02 ,  D01F6/68 ,  Y10S530/858

Abstract: A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

Abstract translation: 本发明的多肽溶液是多肽溶液，其中衍生自天然蜘蛛丝蛋白的多肽溶解在溶剂中。 溶剂含有选自以下（i） - （iii）中的至少一种：（i）DMSO; （ii）具有无机盐的DMSO; 和（iii）具有无机盐的DMF。 此外，在本发明中，通过以下方式获得人造多肽纤维：使用多肽溶液作为掺杂溶液; 将喷丝头溶液从喷丝头挤出到去溶剂浴中，以从溶剂溶液中除去溶剂，并形成纤维以产生未拉伸的纱线。 此外，在本发明中，通过使多肽溶液进行热处理，然后从其中除去未溶解的物质来纯化多肽。 因此，本发明提供了溶质具有高溶解度且溶剂本身具有低成本且能够在高温下溶解并具有高安全性的多肽溶液。人造多肽纤维的制造方法以及纯化多肽的方法。

公开(公告)号：US12163259B2

公开(公告)日：2024-12-10

申请号：US17279083

申请日：2019-09-27

Applicant: Shima Seiki Mfg., Ltd. ,  Spiber Inc.

Inventor： Shozo Torigoe ,  Akihiko Ozeki

IPC: D01D5/06 ,  D01D5/22 ,  D01D10/06 ,  D01F4/02 ,  D01H1/02 ,  D01H3/00 ,  D02G1/00 ,  D02G3/02 ,  D06M11/05 ,  D06M13/144 ,  D06M23/10 ,  D06M101/12

Abstract: An object of the present invention is to provide a method for producing protein spinning capable of securing a stable strength by securing sufficient interlacing between fibers. The method for producing a protein spun yarn of the present invention includes a step (a) of preparing a raw material spun yarn including an uncrimped artificial fibroin fiber containing modified fibroin and a step (b) of bringing the raw material spun yarn into contact with an aqueous medium to crimp the artificial fibroin fiber.

公开(公告)号：US12018056B2

公开(公告)日：2024-06-25

申请号：US16491640

申请日：2018-03-05

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Junichi Sugahara ,  Keisuke Morita ,  Hiroyuki Nakamura

IPC: C07K14/435 ,  D01D10/00 ,  D01F4/02

CPC classification number: C07K14/43518 ,  D01D10/00 ,  D01F4/02

Abstract: The present invention relates to a highly contracted artificial fibroin fiber including a modified fibroin, in which a contraction percentage defined by the following equation exceeds 7%.

Contraction percentage={1−(length of contracted artificial fibroin fiber/length of artificial fibroin fiber before being brought into contact with water after spinning)}×100(%)

公开(公告)号：US20230174997A1

公开(公告)日：2023-06-08

申请号：US17926204

申请日：2021-05-25

Applicant: Spiber Inc.

Inventor： David Lips ,  Gijs Verkleij

IPC: C12N15/76 ,  C12N15/52 ,  C12N15/64 ,  C12N9/10 ,  C12N1/20

CPC classification number: C12N15/76 ,  C12N15/52 ,  C12N15/64 ,  C12N9/1029 ,  C12N1/20 ,  C12R2001/465

Abstract: The present invention relates to a method for preparing a plasmid containing DNA that encodes a Type I polyketide synthase (PKS), the method including a step of introducing a DNA construct containing tandem repeats of DNA that encodes a PKS into a Bacillus subtilis competent cell.