-
公开(公告)号：US11327004B2
公开(公告)日：2022-05-10
申请号：US16082170
申请日：2017-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui Joseph Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
公开(公告)号：US11156619B2
公开(公告)日：2021-10-26
申请号：US16479373
申请日：2018-01-19
Inventor： Liqiang Zhang , Xiangxing Kong , Yanqing Tian , Deirdre Meldrum , Fengyu Su
IPC: G01N21/64 , G01N33/84 , C07D471/16 , C08F220/58 , C12Q1/02 , G01N31/22 , G01N33/50 , G01N21/77
Abstract: Fluorescent pH sensors are provided. The fluorescent pH sensor comprises a copolymer for sensing pH and a polymerized form of N-(2-hydroxypropyl)methacrylamide (HPMA) or 2-hydroxyethyl methacrylate (HEMA). The probe for sensing pH has formula (I): wherein R1 and R2 are as defined herein. The fluorescent pH sensors may be used in determining the pH of a sample and detecting extracellular pH in a sample. Methods for preparing the fluorescent pH sensors and the probe for sensing pH are also provided.
-
公开(公告)号：US10590155B2
公开(公告)日：2020-03-17
申请号：US16314753
申请日：2017-07-14
Inventor： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC: C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
Abstract: Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
-
公开(公告)号：US20200058140A1
公开(公告)日：2020-02-20
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
公开(公告)号：US10471426B2
公开(公告)日：2019-11-12
申请号：US16035464
申请日：2018-07-13
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
-
Patent Agency Ranking
16.发明申请公开(公告)号：US20190126275A1
公开(公告)日：2019-05-02
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
-
公开(公告)号：US20160114323A1
公开(公告)日：2016-04-28
申请号：US14922696
申请日：2015-10-26
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L7/52 , B01L2200/0605 , B01L2200/0642 , B01L2200/0647 , B01L2200/0684 , B01L2300/044 , B01L2300/0636 , B01L2300/0681 , B01L2300/0803 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/0883 , B01L2300/161 , B01L2400/049 , B01L2400/0683
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
Abstract translation: 本发明涉及用于“一锅”分离的化学反应的微流体装置和阵列盘。 阵列盘包括多个扇区，其中每个扇区包括一个微流体装置。 微流体装置包括流体输送通道和孔阵列，其中流体输送通道以蛇形结构输送到孔中。 在一些实施例中，流体输送通道直接在孔阵列的上方。 在其他实施例中，流体输送通道偏离孔阵列，使得从流体输送通道分支的侧通道将流体输送到孔中。 微流体装置的井包括形成地板，井或井的地板或墙壁的至少一部分的透气膜。 在优选实施例中，井是圆柱形的。
-
公开(公告)号：US11369966B2
公开(公告)日：2022-06-28
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
-
公开(公告)号：US11345954B2
公开(公告)日：2022-05-31
申请号：US16323393
申请日：2017-08-07
Inventor： Rhett Martineau , Deirdre Meldrum
IPC: C12Q1/6851 , C12Q1/6853
Abstract: The invention provides methods for detecting specific nucleic acids using a loop-mediated isothermal amplification (LAMP) reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage multiple-threshold array (MTA). During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection. Methods of the invention are especially useful in circumstances where there is lower or intermittent power supply.
-
公开(公告)号：US11315292B2
公开(公告)日：2022-04-26
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
IPC: G06T11/00 , G06T7/194 , G06T7/30 , G01N15/02 , G01N15/14 , G06T5/00 , G06T5/50 , G06T17/00 , G01N15/10
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
-
-
-
-
-
-
-
-
Search Results
28
records
(took 0.023 seconds)
