公开(公告)号：US10569271B2

公开(公告)日：2020-02-25

申请号：US15785727

申请日：2017-10-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERKELEY LIGHTS, INC.

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, Jr. ,  Justin K. Valley

IPC: B01L99/00 ,  B01L3/00 ,  G01N27/447

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

公开(公告)号：US09815056B2

公开(公告)日：2017-11-14

申请号：US14960010

申请日：2015-12-04

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BERKELEY LIGHTS, INC.

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, Jr. ,  Justin K. Valley

IPC: B01L99/00 ,  B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0427 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

公开(公告)号：US11971409B2

公开(公告)日：2024-04-30

申请号：US16892642

申请日：2020-06-04

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang ,  George L. Fox ,  Peggy A. Radel

IPC: B01L3/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC classification number: G01N33/574 ,  B01L3/502761 ,  C07K16/00 ,  C07K16/2887 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54366 ,  G01N33/56972 ,  B01L2200/0647 ,  G01N2800/7028

Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US11964275B2

公开(公告)日：2024-04-23

申请号：US17005116

申请日：2020-08-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Shao Ning Pei ,  Jian Gong ,  Alexander J. Mastroianni ,  Jason M. McEwen ,  Justin K. Valley

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

CPC classification number: B01L3/50273 ,  C12M23/16 ,  C12M29/10 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/161 ,  B01L2400/0427

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.

公开(公告)号：US11801508B2

公开(公告)日：2023-10-31

申请号：US17162467

申请日：2021-01-29

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Alexander J. Mastroianni ,  Mark P. White ,  Gregory G. Lavieu ,  Kristin G. Beaumont

IPC: B01L3/00 ,  C12M3/06 ,  B81C1/00 ,  G01N33/543

CPC classification number: B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B81C1/00206 ,  C12M23/16 ,  G01N33/54393 ,  B01L2300/0636 ,  B01L2300/088 ,  B01L2300/0816 ,  B01L2300/0864 ,  B81B2201/058

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.

公开(公告)号：US11666913B2

公开(公告)日：2023-06-06

申请号：US15359115

申请日：2016-11-22

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Nan-Linda Ding ,  Volker L. S. Kurz ,  Troy A. Lionberger ,  Randall D. Lowe, Jr. ,  Daniele Malleo ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  B01J19/00 ,  G01N27/447

CPC classification number: B01L3/502753 ,  B01J19/0093 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  G01N27/44791 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/0424 ,  B01L2400/0677 ,  B01L2400/08

Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.

公开(公告)号：US10799865B2

公开(公告)日：2020-10-13

申请号：US15336768

申请日：2016-10-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Shao Ning Pei ,  Jian Gong ,  Alexander J. Mastroianni ,  Jason M. McEwen ,  Justin K. Valley

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

公开(公告)号：US20190064038A1

公开(公告)日：2019-02-28

申请号：US16121398

申请日：2018-09-04

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Randall D. Lowe, Jr.

IPC: G01N1/34 ,  G01N27/447 ,  B01L3/00 ,  C12Q1/6806 ,  B03C5/02 ,  B03C5/00

Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.

公开(公告)号：US20170276679A1

公开(公告)日：2017-09-28

申请号：US15406289

申请日：2017-01-13

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan ,  Jason M. McEwen ,  Gang Wang ,  George L. Fox ,  Peggy A. Radel

IPC: G01N33/574 ,  C12Q1/68 ,  G01N33/543 ,  B01L3/00

Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.