公开(公告)号：US20230059461A1

公开(公告)日：2023-02-23

申请号：US18047725

申请日：2022-10-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne

IPC: B01L3/00

Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

公开(公告)号：US11358146B2

公开(公告)日：2022-06-14

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  B01L3/00 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir through which fluid flow is controlled along a fluid path; and wherein the method includes: capturing a population of particles into the array of wells in single-particle format; releasing, from the particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20210283613A1

公开(公告)日：2021-09-16

申请号：US16816817

申请日：2020-03-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne

IPC: B01L3/00

Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

公开(公告)号：US20210003496A1

公开(公告)日：2021-01-07

申请号：US17025326

申请日：2020-09-18

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G06K9/20 ,  G01N35/10 ,  G06K9/00 ,  G01N35/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20250020574A1

公开(公告)日：2025-01-16

申请号：US18887450

申请日：2024-09-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240307880A1

公开(公告)日：2024-09-19

申请号：US18674938

申请日：2024-05-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Austin Payne ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Yi Zhou ,  Saedeh Javdani Sepehri

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L3/5635 ,  B01L7/00 ,  G01N35/0098 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.

公开(公告)号：US20230159917A1

公开(公告)日：2023-05-25

申请号：US18094278

申请日：2023-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  C12N15/1093 ,  G01F23/292 ,  C12N1/06 ,  B01L3/502 ,  B01L3/52 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844 ,  B01L2400/06 ,  B01L2300/123 ,  B01L2200/0647 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/0819 ,  B01L2300/1805 ,  B01L2300/047 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/042 ,  B01L2300/0627 ,  B01L2300/0893 ,  B01L2300/1894 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L2300/16

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US11504714B2

公开(公告)日：2022-11-22

申请号：US16790583

申请日：2020-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US11275015B2

公开(公告)日：2022-03-15

申请号：US16732556

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10 ,  B01L3/02 ,  G06K9/20 ,  G06K9/00 ,  C12M1/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US10914672B2

公开(公告)日：2021-02-09

申请号：US17025326

申请日：2020-09-18

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/10 ,  G01N15/14 ,  G01N1/20 ,  B01L3/00 ,  C12M1/00 ,  G01N1/28 ,  G01N1/40 ,  G06K9/20 ,  G01N35/10 ,  G06K9/00 ,  G01N35/00 ,  B01L3/02 ,  G01N15/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.