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    Absolute quantification of nucleic acids and related methods and systems
    12.
    发明授权
    Absolute quantification of nucleic acids and related methods and systems 有权

    公开(公告)号：US12173363B2

    公开(公告)日：2024-12-24

    申请号：US17863183

    申请日：2022-07-12

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： Rustem F. Ismagilov ,  Jacob T. Barlow ,  Said R. Bogatyrev

    IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6888

    Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

    Methods and systems and related compositions for mixtures separation with a solid matrix
    14.
    发明授权
    Methods and systems and related compositions for mixtures separation with a solid matrix 有权

    公开(公告)号：US11866695B2

    公开(公告)日：2024-01-09

    申请号：US17131449

    申请日：2020-12-22

    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

    Inventor： Rustem F. Ismagilov ,  Erik Jue ,  Daan Witters

    IPC: C12N15/10 ,  C12Q1/6806

    CPC classification number: C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

    Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.

    Freeze-cast ceramic membrane for size based filtration
    17.
    发明授权
    Freeze-cast ceramic membrane for size based filtration 有权

    公开(公告)号：US12157705B2

    公开(公告)日：2024-12-03

    申请号：US18228369

    申请日：2023-07-31

    Applicant: California Institute of Technology

    Inventor： Katherine T. Faber ,  Julia A. Kornfield ,  Noriaki Arai ,  Orland Bateman ,  Rustem F. Ismagilov

    IPC: C04B38/06 ,  B01D67/00 ,  B01D69/02 ,  B01D71/02 ,  B01J35/56 ,  B01J35/64 ,  C04B38/00 ,  H01B1/14 ,  H01B1/16

    Abstract: Provided herein are methods for making a freeze-cast material having a internal structure, the methods comprising steps of: determining the internal structure of the material, the internal structure having a plurality of pores, wherein: each of the plurality of pores has directionality; and the step of determining comprises: selecting a temperature gradient and a freezing front velocity to obtain the determined internal structure based on the selected temperature gradient and the selected freezing front velocity; directionally freezing a liquid formulation to form a frozen solid, the step of directionally freezing comprising: controlling the temperature gradient and the freezing front velocity to match the selected temperature gradient and the selected freezing front velocity during directionally freezing; wherein the liquid formulation comprises at least one solvent and at least one dispersed species; and subliming the at least one solvent out of the frozen solid to form the material.

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