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    Nucleic acid quantification products and processes
    13.
    发明授权
    Nucleic acid quantification products and processes 有权

    公开(公告)号：US10533215B2

    公开(公告)日：2020-01-14

    申请号：US13129797

    申请日：2009-11-20

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68 ,  C12Q1/6837

    Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

    Methods and compositions for the analysis of nucleic acids
    14.
    发明授权
    Methods and compositions for the analysis of nucleic acids 有权
    用于分析核酸的方法和组合物

    公开(公告)号：US08852864B2

    公开(公告)日：2014-10-07

    申请号：US12863169

    申请日：2009-01-16

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

    Abstract: Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analysis on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

    Abstract translation: 本文提供了用于核酸分析的组合物和方法，包括用于基因分型，单倍型，测序和对核酸进行其他遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。

    Solid phase sequencing of biopolymers
    16.
    发明授权
    Solid phase sequencing of biopolymers 失效
    生物聚合物的固相测序

    公开(公告)号：US08758995B2

    公开(公告)日：2014-06-24

    申请号：US12852336

    申请日：2010-08-06

    Applicant: Charles R. Cantor ,  Hubert Koster

    Inventor： Charles R. Cantor ,  Hubert Koster

    IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC classification number: C12Q1/6872 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2531/113 ,  C12Q2521/525 ,  C12Q2565/627 ,  C12Q2531/119 ,  C12Q2523/301 ,  C12Q2531/137 ,  C12Q2521/301 ,  C12Q2531/149

    Abstract: This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

    Abstract translation: 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 探针可以固定在诸如杂交芯片的固体支持物上，以便于自动化分子量分析和目标序列的鉴定。

    Haplotype Analysis
    17.
    发明申请
    Haplotype Analysis 有权
    单倍型分析

    公开(公告)号：US20120295263A1

    公开(公告)日：2012-11-22

    申请号：US13565105

    申请日：2012-08-02

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: G01N27/62

    CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

    Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

    Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

    METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS
    18.
    发明申请
    METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS 审中-公开
    非侵入性预后诊断方法

    公开(公告)号：US20120225798A1

    公开(公告)日：2012-09-06

    申请号：US13369000

    申请日：2012-02-08

    Applicant: Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

    Inventor： Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

    IPC: C40B30/10

    CPC classification number: C12Q1/6881 ,  C12Q1/6872 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2535/125

    Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.

    Abstract translation: 本发明涉及检测生物样品中核酸的方法。 该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。 该方法可用于例如诊断，预后和治疗目的。 该方法允许精确检测在生物样品中以非常少的量存在的核酸。 例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性核酸。 在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。

    Nucleic acid supported protein complementation
    19.
    发明授权
    Nucleic acid supported protein complementation 有权
    核酸支持的蛋白质互补

    公开(公告)号：US07999095B2

    公开(公告)日：2011-08-16

    申请号：US12638871

    申请日：2009-12-15

    Applicant: Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    Inventor： Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    IPC: C07H21/04 ,  C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

    Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

    Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

    NUCLEIC ACID SUPPORTED PROTEIN COMPLEMENTATION
    20.
    发明申请
    NUCLEIC ACID SUPPORTED PROTEIN COMPLEMENTATION 有权
    核酸支持蛋白质补充

    公开(公告)号：US20100297629A1

    公开(公告)日：2010-11-25

    申请号：US12638871

    申请日：2009-12-15

    Applicant: Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    Inventor： Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    IPC: C12Q1/68 ,  C07K9/00

    CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

    Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

    Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

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