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    Solid phase sequencing of double-stranded nucleic acids
    11.
    发明授权
    Solid phase sequencing of double-stranded nucleic acids 失效
    双链核酸的固相测序

    公开(公告)号：US06436635B1

    公开(公告)日：2002-08-20

    申请号：US08614151

    申请日：1996-03-12

    Applicant: Dong-Jing Fu ,  Charles R. Cantor ,  Hubert Köster ,  Cassandra L. Smith

    Inventor： Dong-Jing Fu ,  Charles R. Cantor ,  Hubert Köster ,  Cassandra L. Smith

    IPC: C12Q168

    CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6872 ,  C40B40/06 ,  C12Q2565/537 ,  C12Q2525/161 ,  C12Q2565/501 ,  C12Q2565/627

    Abstract: This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

    Abstract translation: 本发明涉及用于检测和测序靶双链核酸序列，用于这些方法的核酸探针和探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包含在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 可以确定其序列的核酸包括生物样品中的核酸，例如患者活组织检查和环境样品。 探针可以固定在固体支持物例如杂交芯片上以促进分子量的自动测定和靶序列的鉴定。

    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers
    13.
    发明授权
    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers 失效
    双特异性试剂的设计和合成：使用双链DNA作为化学和空间定义的交联剂

    公开(公告)号：US5849878A

    公开(公告)日：1998-12-15

    申请号：US481065

    申请日：1995-06-07

    Applicant: Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    Inventor： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    IPC: C07K16/28 ,  C07K16/46 ,  C07K17/00 ,  C07K19/00

    CPC classification number: C07K16/2812 ,  C07K16/2809 ,  C07K16/468

    Abstract: The invention relates to bis-protein-DNA conjugates. A protein having a specific ligand binding activity is covalently linked to each end of a derivatized DNA molecule. These bis-protein-DNA conjugates can be used for immunoassays, PCR assays and measuring distances between proteins at up to 3.4 A resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be conjugated to the proteins, including antibodies or Fab' fragments, using disulfide bond linkage.

    Abstract translation: 本发明涉及双蛋白-DNA缀合物。 具有特异性配体结合活性的蛋白质与衍生的DNA分子的每个末端共价连接。 这些双蛋白-DNA缀合物可用于免疫测定，PCR测定和测量蛋白质之间的距离，高达3.4A分辨率。 本发明还涉及合成这些双蛋白-DNA缀合物的方法。 共轭物的合成需要衍生DNA寡核苷酸的5'或3'末端并共价连接该DNA与蛋白质。 使用二硫键可将DNA与蛋白质缀合，包括抗体或Fab'片段。

    Nucleic acid quantification products and processes
    16.
    发明授权
    Nucleic acid quantification products and processes 有权

    公开(公告)号：US10533215B2

    公开(公告)日：2020-01-14

    申请号：US13129797

    申请日：2009-11-20

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68 ,  C12Q1/6837

    Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

    Methods and compositions for the analysis of nucleic acids
    17.
    发明授权
    Methods and compositions for the analysis of nucleic acids 有权
    用于分析核酸的方法和组合物

    公开(公告)号：US08852864B2

    公开(公告)日：2014-10-07

    申请号：US12863169

    申请日：2009-01-16

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

    Abstract: Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analysis on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

    Abstract translation: 本文提供了用于核酸分析的组合物和方法，包括用于基因分型，单倍型，测序和对核酸进行其他遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。

    Solid phase sequencing of biopolymers
    19.
    发明授权
    Solid phase sequencing of biopolymers 失效
    生物聚合物的固相测序

    公开(公告)号：US08758995B2

    公开(公告)日：2014-06-24

    申请号：US12852336

    申请日：2010-08-06

    Applicant: Charles R. Cantor ,  Hubert Koster

    Inventor： Charles R. Cantor ,  Hubert Koster

    IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC classification number: C12Q1/6872 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2531/113 ,  C12Q2521/525 ,  C12Q2565/627 ,  C12Q2531/119 ,  C12Q2523/301 ,  C12Q2531/137 ,  C12Q2521/301 ,  C12Q2531/149

    Abstract: This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

    Abstract translation: 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 探针可以固定在诸如杂交芯片的固体支持物上，以便于自动化分子量分析和目标序列的鉴定。

    Haplotype Analysis
    20.
    发明申请
    Haplotype Analysis 有权
    单倍型分析

    公开(公告)号：US20120295263A1

    公开(公告)日：2012-11-22

    申请号：US13565105

    申请日：2012-08-02

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: G01N27/62

    CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

    Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

    Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

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