公开(公告)号：US20160305969A1

公开(公告)日：2016-10-20

申请号：US15032121

申请日：2014-10-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/94 ,  C07C251/22 ,  C07B59/00 ,  C07D231/46

CPC classification number: G01N33/9406 ,  C07B59/001 ,  C07B59/002 ,  C07B2200/05 ,  C07C251/22 ,  C07C2601/16 ,  C07D231/46 ,  G01N2560/00

Abstract: A method is described for mass spectrometric analysis, detection and quantification of catecholamines. The methods can comprise reacting the catecholamines with a 4-aminoantippyrine reagent and detecting and/or quantifying the adduct produced by the reaction. The methods can also allow for multiplexing. Compounds formed by the reactions are also provided.

Abstract translation: 描述了用于质谱分析，儿茶酚胺检测和定量的方法。 所述方法可以包括使儿茶酚胺与4-氨基嘌呤试剂反应，并检测和/或定量由反应产生的加合物。 这些方法也可以允许复用。 还提供了由反应形成的化合物。

公开(公告)号：US20150140600A1

公开(公告)日：2015-05-21

申请号：US14602917

申请日：2015-01-22

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Brian L. Williamson ,  Subhasish Purkayastha

IPC: C12Q1/26 ,  G01N33/50

CPC classification number: C12Q1/26 ,  C12N9/0077 ,  G01N30/7233 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/5067 ,  G01N33/6848 ,  G01N2030/8831 ,  G01N2333/90245 ,  G01N2500/04 ,  G01N2500/10 ,  G01N2500/20 ,  G01N2560/00 ,  Y10T436/24

Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.

Abstract translation: 提供了用于筛选细胞色素P450（CYP）诱导的药物的方法，并且可以包括将药物与含有微粒体的生物样品孵育，然后定量至少一种细胞色素P450同种型。 同种型可以选自2B6,3A4,1A2和3A5同种型。 在一些实施方案中，该方法使用液相色谱串联质谱（LC-MSMS）。 可以将定量值与阈值进行比较，并且当定量值不超过阈值时，可以确定药物表现出可接受的CYP诱导电位。 还提供了分离的肽。

公开(公告)号：US20250027950A1

公开(公告)日：2025-01-23

申请号：US18630611

申请日：2024-04-09

Applicant: BECKMAN COULTER, INC. ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Hudson ,  Takayuki Mizutani ,  Subhasish Purkayastha ,  Thomas W. Roscoe

IPC: G01N33/68 ,  G01N27/624 ,  H01J49/00 ,  H01J49/02

Abstract: An integrated sample processing system including an analyzer and a mass spectrometer is disclosed. The integrated sample processing system can perform multiple different types of detection, thereby providing improved flexibility and better accuracy in processing samples. The detection systems in the sample processing system may include an optical detection system and a mass spectrometer.

公开(公告)号：US11619623B2

公开(公告)日：2023-04-04

申请号：US16956405

申请日：2018-12-19

Applicant: Beckman Coulter, Inc. ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Hudson ,  Takayuki Mizutani ,  Subhasish Purkayastha ,  Thomas W. Roscoe

IPC: G01N33/487 ,  G01N35/00 ,  G01N35/10 ,  G01N35/04 ,  H01J49/26

Abstract: One embodiment of the invention is directed to a sample processing system for analyzing a biological sample from a patient. The sample processing system comprises: a plurality of analyzers comprising at least one mass spectrometer, wherein each analyzer in the plurality of analyzers is configured to acquire at least one measurement value corresponding to at least one characteristic of the biological sample; at least one data storage component which stores (i) a list of parameters for the plurality of analyzers, and (ii) at least two condition sets, which contain data associated with completing one or more test orders. The condition sets contain data which differ by at least one variable; and a control system operatively coupled to the plurality of analyzers, and the at least one data storage component. The control system is configured to (i) determine which condition set of the at least two condition sets to use based on the determined condition set, (ii) determine which analyzer or analyzers of the plurality of analyzers to use to process each test order based on the determined condition set and one or more parameters from the list of parameters, and (iii) cause the determined analyzer or analyzers to acquire one or more measurement values for the biological sample.

公开(公告)号：US20190212337A1

公开(公告)日：2019-07-11

申请号：US16358533

申请日：2019-03-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  G01N33/68 ,  G01N30/88 ,  G01N30/72 ,  C12Q1/686 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/68 ,  C12Q1/686 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

公开(公告)号：US10078091B2

公开(公告)日：2018-09-18

申请号：US14401427

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd. ,  Oregon Health and Science University

Inventor： Andrea DeBarber ,  Subhasish Purkayastha ,  Robert D. Steiner ,  Michal Weinstock

IPC: G01N33/00 ,  G01N33/92 ,  G01N33/58 ,  H01J49/00 ,  G01N33/68

CPC classification number: G01N33/92 ,  G01N33/58 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2333/90251 ,  G01N2400/00 ,  G01N2800/04 ,  G01N2800/065 ,  G01N2800/385 ,  H01J49/0031

Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the analysis of ketosterol biomarkers such as bile acid precursors from human plasma, serum or whole blood. This method is used for new born screening for Cerebrotendinous Xanthomatosis (CTX). Methods for labeling, analyzing, and quantifying ketosterol biomarkers are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US09874575B2

公开(公告)日：2018-01-23

申请号：US14367230

申请日：2013-01-16

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/74 ,  G01N33/58 ,  G01N30/72

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2560/00 ,  Y10T436/18 ,  Y10T436/200833 ,  Y10T436/203332

Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.

公开(公告)号：US20170115258A1

公开(公告)日：2017-04-27

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N30/72 ,  G01N33/74

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US09500622B2

公开(公告)日：2016-11-22

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N27/62 ,  G01N30/72 ,  G01N33/64 ,  G01N33/74 ,  G01N30/04 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US09188596B2

公开(公告)日：2015-11-17

申请号：US14104117

申请日：2013-12-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。