公开(公告)号：US20240207839A1

公开(公告)日：2024-06-27

申请号：US18542470

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Brian D. Mather ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/165

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a composite that includes a thermo-responsive polymer and a photo-thermal additive.

公开(公告)号：US20230295455A1

公开(公告)日：2023-09-21

申请号：US18013188

申请日：2021-10-19

Applicant: ILLUMINA, INC.

Inventor： Raymond Campos ,  Brian D. Mather ,  Sean M. Ramirez

IPC: C09D133/24

CPC classification number: C09D133/24

Abstract: A flow cell includes a substrate and a copolymer coating. The copolymer coating includes copolymer chains, each having recurring units of formula (I): and formula (II):. In formula (I), R1 is —H, a halogen, an alkyl, an alkoxy, an alkenyl, an alkynyl, a cycloalkyl, an aryl, a heteroaryl, a heterocycle, or optionally substituted variants thereof; R2 is an azido; each (CH2)p can be optionally substituted; and p is an integer from 1 to 50. In formula (II), each of R3, R3′, R4, R4′ is —H, R5, —OR5, —C(O)OR5, —C(O)R5, —OC(O)R5, —C(O)NR6R7, or —NR6R7; R5 is —H, —OH, an alkyl, a cycloalkyl, a hydroxyalkyl, an aryl, a heteroaryl, a heterocycle, or optionally substituted variants thereof; and each of R6 and R7 is —H or an alkyl. Some copolymer chains include at least one alkoxyamine end group.

公开(公告)号：US20220154273A1

公开(公告)日：2022-05-19

申请号：US17525530

申请日：2021-11-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Mathieu Lessard-Viger ,  Brian D. Mather ,  Seth M. McDonald ,  Kaitlin M. Pugliese ,  Xavier von Hatten

IPC: C12Q1/6874 ,  B01L3/00

Abstract: An example of an incorporation mix includes a liquid carrier, a complex, and a labeled nucleotide. The complex includes a polymerase and a plasmonic nanostructure linked to the polymerase. The labeled nucleotide includes a nucleotide, a 3′ OH blocking group attached to a sugar of the nucleotide, and a dye label attached to a base of the nucleotide.

公开(公告)号：US20220145379A1

公开(公告)日：2022-05-12

申请号：US17599994

申请日：2020-11-25

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Brian D. Mather ,  Andrew A. Brown ,  Pietro Gatti Lafranconi ,  Maria Candelaria Rogert Bacigalupo ,  Antoine Francais ,  Xiaohai Liu

IPC: C12Q1/6848 ,  C12Q1/6869

Abstract: An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

公开(公告)号：US20200325467A1

公开(公告)日：2020-10-15

申请号：US16626221

申请日：2018-06-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Brian D. Mather ,  Cyril Delattre ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Pallavi Daggumati ,  Behnam Javanmardi ,  Filiz Gorpe-Yasar ,  Sebastien Georg Gabriel Ricoult ,  Xavier von Hatten ,  Daniel Leonard Fuller

IPC: C12N15/10 ,  C08F226/10

Abstract: An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.

公开(公告)号：US20180327832A1

公开(公告)日：2018-11-15

申请号：US15969562

申请日：2018-05-02

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Sean M. Ramirez ,  Brian D. Mather ,  Edwin Li ,  Sojeong Moon ,  Innsu Daniel Kim ,  Alexandre Richez ,  Ludovic Vincent ,  Xavier von Hatten ,  Hai Quang Tran ,  Maxwell Zimmerley ,  Julia Morrison ,  Gianluca Andrea Artioli ,  Krystal Sly ,  Hayden Black ,  Lewis J. Kraft ,  Hong Xie ,  Wei Wei ,  Ryan Sanford

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806

Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

公开(公告)号：US20230416435A1

公开(公告)日：2023-12-28

申请号：US18330298

申请日：2023-06-06

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Timothy J.N. Beech ,  Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Brian D. Mather ,  Weixian Xi ,  Xavier von Hatten

IPC: C08F220/56 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C08F220/56 ,  C08J3/075 ,  C12Q1/6869 ,  C08J2333/26

Abstract: A co-polymer includes a plurality of a first monomer including a terminal functional group that is to attach to at least two different primers; a plurality of a second monomer including a second functional group that is different from the terminal functional group, and that is selected from the group consisting of a phenyl group, methoxy propyl, glycosyl, vinyl pyrrolidone, and an imidazole group; and a plurality of a third monomer that is different from the first and second monomers. This co-polymer may be used in a flow cell, and may enhance the clustering efficiency and kinetics.

公开(公告)号：US20230272469A1

公开(公告)日：2023-08-31

申请号：US18195321

申请日：2023-05-09

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Sean M. Ramirez ,  Brian D. Mather ,  Edwin Li ,  Sojeong Moon ,  Innsu Daniel Kim ,  Alexandre Richez ,  Ludovic Vincent ,  Xavier von Hatten ,  Hai Quang Tran ,  Maxwell Zimmerley ,  Julia Morrison ,  Gianluca Andrea Artioli ,  Krystal Sly ,  Hayden Black ,  Lewis J. Kraft ,  Hong Xie ,  Wei Wei ,  Ryan Sanford

IPC: C12Q1/6874 ,  C12Q1/6806 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  B01L3/502707 ,  B01L2300/165 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/0877

Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

公开(公告)号：US11384392B2

公开(公告)日：2022-07-12

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20220154177A1

公开(公告)日：2022-05-19

申请号：US17525553

申请日：2021-11-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Mathieu Lessard-Viger ,  Brian D. Mather ,  Xavier von Hatten

IPC: C12N15/10 ,  C08F265/10 ,  C08J3/075

Abstract: An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.