公开(公告)号：US11846580B2

公开(公告)日：2023-12-19

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  G01N15/10 ,  B01J19/00

CPC classification number: G01N15/1436 ,  B01J19/0046 ,  B01L3/502 ,  B01L3/502707 ,  C09D133/24 ,  C09D133/26 ,  C12Q1/6874 ,  G01N15/1484 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00418 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00722 ,  B01L2200/12 ,  B01L2300/087 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/0887 ,  B01L2300/16 ,  G01N2015/1006

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US11834704B2

公开(公告)日：2023-12-05

申请号：US17113180

申请日：2020-12-07

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/6844 ,  C40B50/18 ,  C12Q1/686 ,  B01J19/00 ,  C40B40/06

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  C12Q1/686 ,  C40B50/18 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C40B40/06 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US10280454B2

公开(公告)日：2019-05-07

申请号：US15470577

申请日：2017-03-27

Applicant: Illumina, Inc.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/68 ,  B01J19/00 ,  C40B40/06 ,  C12Q1/6844 ,  C40B50/18 ,  C12Q1/686

Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

公开(公告)号：US20180371535A1

公开(公告)日：2018-12-27

申请号：US16118268

申请日：2018-08-30

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/6844 ,  C12Q1/686 ,  B01J19/00 ,  C40B50/18

Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US09670535B2

公开(公告)日：2017-06-06

申请号：US14194143

申请日：2014-02-28

Applicant: Illumina, Inc.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C40B50/18 ,  C12Q1/68 ,  B01J19/00

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

公开(公告)号：US08778849B2

公开(公告)日：2014-07-15

申请号：US13661524

申请日：2012-10-26

Applicant: Illumina, Inc.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C40B50/18 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

Abstract translation: 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。

公开(公告)号：US20210291135A1

公开(公告)日：2021-09-23

申请号：US17338576

申请日：2021-06-03

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

公开(公告)号：US20190046943A1

公开(公告)日：2019-02-14

申请号：US16118307

申请日：2018-08-30

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.

公开(公告)号：US20170197193A1

公开(公告)日：2017-07-13

申请号：US15470577

申请日：2017-03-27

Applicant: Illumina, Inc.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: B01J19/00 ,  C12Q1/68 ,  C40B50/18

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

公开(公告)号：US09512422B2

公开(公告)日：2016-12-06

申请号：US13787396

申请日：2013-03-06

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

Abstract translation: 提供了一种阵列，其包括具有表面的固体支持物，所述表面具有多个孔，所述孔包含凝胶材料，所述孔通过表面上的间隙区彼此分离，所述间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 和凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。