公开(公告)号：US20160257966A1

公开(公告)日：2016-09-08

申请号：US15057551

申请日：2016-03-01

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Youngsoon UM ,  Jun Won CHWA ,  Gyeongtaek GONG

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12N9/88 ,  C12N9/13 ,  C12N9/93 ,  C12Y208/03009 ,  C12Y401/01004 ,  C12Y602/01001 ,  Y02P60/247

Abstract: Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

Abstract translation: 本文公开了具有改进的丙酮生产能力的转化的聚球藻细菌菌株和用于生产丙酮的方法，以及使用该方法除去二氧化碳的方法。 在一方面，本公开的转化的细小球藻菌株可以使用二氧化碳作为碳源以高选择性产生丙酮。 本公开是经济的，因为细菌聚球藻菌株可以使用存在于大气中的二氧化碳作为碳源经济地生产高附加值的丙酮，而不需要额外的催化反应。 此外，本公开是环境友好的，因为可以使用微生物除去或减少大气中的二氧化碳。

公开(公告)号：US20230242948A1

公开(公告)日：2023-08-03

申请号：US17823034

申请日：2022-08-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi LEE ,  Yeon-Ho CHOI ,  Gyeongtaek GONG ,  Ja Kyoung KO ,  Jung Ho AHN ,  Youngsoon UM

IPC: C12P7/6409 ,  C12N9/10 ,  C12N1/16

CPC classification number: C12P7/6409 ,  C12N9/1029 ,  C12Y203/0102 ,  C12N1/16

Abstract: The present specification describes a culture of a microorganism, which comprises an increased content of oleic acid, or a microbial oil comprising the same. In addition, the present specification describes a method for producing oleic acid and lipids comprising the same by culturing a microorganism. Since the present disclosure enables the production of lipids comprising oleic acid at a high concentration without genetic manipulation of a lipid-producing microorganism, it may be utilized in various industrial fields requiring oleic acid, such as foods, cosmetic materials, biofuels, etc.

公开(公告)号：US20190226036A1

公开(公告)日：2019-07-25

申请号：US16253581

申请日：2019-01-22

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Youngsoon UM ,  Joongsuk KIM ,  Sun-Mi LEE ,  Gyeongtaek GONG

IPC: C12R1/145 ,  C12P7/16 ,  C12P7/52

Abstract: The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

公开(公告)号：US20170283832A1

公开(公告)日：2017-10-05

申请号：US15357815

申请日：2016-11-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Sun-young CHOI ,  Youngsoon UM ,  Gyeong Taek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12P5/00 ,  C12N9/90 ,  C12N9/10 ,  C12N15/52 ,  C12N9/04

CPC classification number: C12P5/007 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01267 ,  C12Y202/01007 ,  C12Y205/0101 ,  C12Y205/01021 ,  C12Y503/03002

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

公开(公告)号：US20170114375A1

公开(公告)日：2017-04-27

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Hyun Jeong LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12P7/64 ,  C12N1/20

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01001 ,  C12Y401/01001

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US20170009240A1

公开(公告)日：2017-01-12

申请号：US14950425

申请日：2015-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Eun Mi KIM ,  Youngsoon UM ,  Gyeongtaek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12N15/52 ,  C12N15/71 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04 ,  C12N9/12 ,  C12N15/72 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C12Y101/01034 ,  C12Y203/01026 ,  C12Y205/01 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y401/03 ,  C12Y503/03002

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

Abstract translation: 本文公开了能够表达月桂烯的表达载体，用载体转化的大肠杆菌菌株，并且具有改进的生产月桂烯的能力和用于制备月桂烯的方法和使用该方法回收甘油的方法。 在一方面，本公开的转化的大肠杆菌菌株可以使用甘油或葡萄糖作为碳源大量生产高纯度的月桂烯。 此外，本公开的大肠杆菌菌株是经济和环境友好的，因为它可以使用废甘油作为碳源产生高附加值的月桂烯。 此外，强烈挥发性月桂烯可以同时生产和分离。

公开(公告)号：US20160298146A1

公开(公告)日：2016-10-13

申请号：US15092829

申请日：2016-04-07

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon UM ,  Yunje KIM ,  Kyung Min LEE ,  Han Min WOO ,  Gyeongtaek GONG ,  Ki Yeon KIM

IPC: C12P7/52

CPC classification number: C12P7/52 ,  C12P19/02 ,  C12P19/12 ,  C12P2203/00 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

Abstract translation: 本公开涉及木质纤维素生物质和海藻生物质的混合物的水解产物。 通过将海藻生物质与木质纤维素生物质混合，然后制备水解产物，木质纤维素生物质衍生的乙酸与海藻生物质衍生的甘露醇一起消耗。 因此，可以在减少发酵抑制剂的同时保持高的糖生产率。 因为本公开可以解决由于木素纤维素衍生的发酵抑制剂引起的发酵效率降低的木质纤维素生物质的问题和尽管发酵时间长的生产效率非常低的海藻生物质的问题，但根据本公开的水解产物可用于 经济地生产生物燃料和生物化学品。

公开(公告)号：US20160115491A1

公开(公告)日：2016-04-28

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Youngsoon UM ,  Yunje KIM ,  Gyeong Taek GONG ,  Jung Seok LEE

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。

公开(公告)号：US20160002677A1

公开(公告)日：2016-01-07

申请号：US14494930

申请日：2014-09-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jung Seok LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Byoung Koun MIN ,  Jeong-Myeong HA ,  Yunje KIM ,  Sang Jun SIM

IPC: C12P7/46 ,  C12N15/77

CPC classification number: C12P7/46 ,  C12N15/77

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract translation: 本公开涉及使用在二氧化碳作为直接碳源而不将其转化为葡萄糖而生长的微藻中积累的淀粉生产琥珀酸的菌株，以及使用该淀粉生产琥珀酸盐的方法。 本公开提供了从二氧化碳生产琥珀酸盐的菌株，其选自谷氨酸棒杆菌BL-1-pBlAmyS（KCTC 12585BP）和谷氨酸棒杆菌BL-1-pSbAmyA（KCTC 12587BP）。 本公开还提供了一种从二氧化碳生产琥珀酸的方法，包括通过在含淀粉培养基中从二氧化碳接种产生琥珀酸的菌株来发酵淀粉。

公开(公告)号：US20150275187A1

公开(公告)日：2015-10-01

申请号：US14338770

申请日：2014-07-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Hong-Sil PARK ,  Gyeong Taek Gong ,  Youngsoon UM ,  Yunje KIM ,  Dong Jin SUH

IPC: C12N9/02 ,  C12Q1/68 ,  C12P13/00

CPC classification number: C12Q1/6876 ,  C12N9/0006 ,  C12P13/04 ,  C12Q1/689 ,  C12Q2600/142 ,  C12Y101/03021 ,  C12Y103/01032

Abstract: The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene.

Abstract translation: 含有根据本公开的糠醛抗性基因的糠醛抗性菌株可以有效地在含糠醛的培养基中生长。 因此，可以解决微生物发酵困难的问题，因为衍生自不可食用的木质纤维素生物质的水解产物中含有糠醛等有毒副产物。 此外，根据本公开的菌株的制造方法，抗性基因可以选自相对少量的靶基因。 因此，可以节省开发耐药菌株的时间，成本等。 此外，用于鉴定基因的方法可广泛地应用于除了糠醛抗性基因之外鉴定各种未知功能基因的方法。