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    NUCLEIC ACID AMPLIFICATION
    11.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190119738A1

    公开(公告)日：2019-04-25

    申请号：US16168810

    申请日：2018-10-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan M. ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853

    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING
    14.
    发明申请
    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 审中-公开
    SCAFFEDED核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20160194629A1

    公开(公告)日：2016-07-07

    申请号：US14987552

    申请日：2016-01-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

    IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    18.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开

    公开(公告)号：US20160011145A1

    公开(公告)日：2016-01-14

    申请号：US14863718

    申请日：2015-09-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: G01N27/414 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    19.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开
    用于生物和化学分析的集成传感器阵列

    公开(公告)号：US20150197797A1

    公开(公告)日：2015-07-16

    申请号：US14599948

    申请日：2015-01-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

    Abstract translation: 公开了一种在电路支撑衬底中包括化学场效应晶体管阵列的装置。 晶体管阵列在其表面上设置有能够从样品流体中保留化学或生物样品的样品保持区阵列。 晶体管阵列具有10μm或更小的间距，并且样品保持区位于至少一个化学场效应晶体管上，该化学场效应晶体管被配置为产生与该样品中的化学或生物样品的特征相关的至少一个输出信号 - 区域。

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