公开(公告)号：US20230220414A1

公开(公告)日：2023-07-13

申请号：US17934981

申请日：2022-09-23

申请人： INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

发明人： Marisol CORRAL-DEBRINSKI ,  Jose-Alain SAHEL ,  Valerie KALTIMBACHER ,  Crystel BONNET

IPC分类号： C12N15/85 ,  C12N9/02 ,  A61K48/00 ,  C07K14/47 ,  C12N9/10

CPC分类号： C12N15/85 ,  C12N9/0089 ,  A61K48/0058 ,  A61K48/0066 ,  C07K14/47 ,  C12N9/0053 ,  C12N15/8509 ,  A61K48/005 ,  C12Y109/03001 ,  C12N9/1085 ,  C12N2810/80 ,  C12N2800/22 ,  C07K2319/07

摘要： An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.

公开(公告)号：US20180346985A1

公开(公告)日：2018-12-06

申请号：US16057052

申请日：2018-08-07

申请人： MDNA Life Sciences Inc.

发明人： Andrew Harbottle ,  Gabriel Dakubo ,  Ryan L. Parr ,  Jennifer Creed ,  Brian Reguly ,  Kerry Robinson

IPC分类号： C12Q1/6883 ,  C12N9/12 ,  C12N9/02 ,  C07K14/80 ,  C12N15/62 ,  C12N9/14

CPC分类号： C12Q1/6883 ,  C07K14/80 ,  C07K2319/00 ,  C12N9/0036 ,  C12N9/0053 ,  C12N9/12 ,  C12N9/14 ,  C12N15/62 ,  C12Q2600/148 ,  C12Q2600/156 ,  C12Y106/99003 ,  C12Y109/03001 ,  C12Y207/10001 ,  C12Y306/01003

摘要： The present invention provides novel mitochondrial fusion transcripts and related deletion molecules that are associated with UV exposure. Methods for in vivo and in vitro detection of mtDNA molecules and associated fusion transcripts is also provided, as is their use in the screening and testing of skin care products.

公开(公告)号：US09982242B2

公开(公告)日：2018-05-29

申请号：US14441657

申请日：2013-11-08

申请人： SANYO FOODS CO., LTD.

发明人： Hiroyuki Nagaoka

IPC分类号： A61K8/64 ,  A61L27/04 ,  C12N9/04 ,  C12P41/00 ,  C12P7/22 ,  C12N9/02 ,  C12N9/08

CPC分类号： C12N9/0006 ,  C12N9/0053 ,  C12N9/0065 ,  C12P7/22 ,  C12P41/002 ,  C12Y101/05 ,  C12Y109/03001 ,  C12Y111/01

摘要： Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.

公开(公告)号：US09827311B2

公开(公告)日：2017-11-28

申请号：US14802616

申请日：2015-07-17

申请人： L'OREAL

发明人： Julien Laboureau ,  Quang Lan Nguyen ,  Dang-Man Pham

IPC分类号： A61K8/66 ,  A61N5/06 ,  A61K41/00 ,  A61K8/19 ,  A61K8/49 ,  A61Q7/00 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/08 ,  A61K38/17 ,  A61K45/06

CPC分类号： A61K41/00 ,  A61K8/19 ,  A61K8/494 ,  A61K8/66 ,  A61K38/1709 ,  A61K45/06 ,  A61K2800/81 ,  A61K2800/92 ,  A61N5/0617 ,  A61Q7/00 ,  A61Q19/007 ,  A61Q19/02 ,  A61Q19/08 ,  C12Y109/03001

摘要： The present invention relates more specifically to a cosmetic treatment method intended in particular to improve the appearance of the skin and/or hair comprising the simultaneous and/or sequential administration: a) of at least one cytochrome C oxidase substrate and/or of at least one agent which increases the expression of the said substrate; and b) of at least one light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase. In particular, the said light radiation exhibits at least one predominant wavelength which activates cytochrome C oxidase ranging from 550 to 1000 nm, in particular from 550 to 800 nm, preferably from 620 to 700 nm and more preferably still from 640 to 680 nm and is preferably used at a dose ranging from 0.01 to 200 J/cm2, preferably from 0.1 to 30 J/cm2, more preferably from 1 to 30 J/cm2, indeed even from 5 to 30 J/cm2.The invention also relates to a composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and at least one compound emitting and/or filtering, in particular under exposure to light, a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase, and to a kit comprising at least one composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and one device and/or one compound emitting/filtering a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase.

公开(公告)号：US20160015812A1

公开(公告)日：2016-01-21

申请号：US14802616

申请日：2015-07-17

申请人： L'OREAL

发明人： Julien LABOUREAU ,  Quang Lan Nguyen ,  Dang-Man Pham

IPC分类号： A61K41/00 ,  A61K45/06 ,  A61K38/17

CPC分类号： A61K41/00 ,  A61K8/19 ,  A61K8/494 ,  A61K8/66 ,  A61K38/1709 ,  A61K45/06 ,  A61K2800/81 ,  A61K2800/92 ,  A61N5/0617 ,  A61Q7/00 ,  A61Q19/007 ,  A61Q19/02 ,  A61Q19/08 ,  C12Y109/03001

摘要： The present invention relates more specifically to a cosmetic treatment method intended in particular to improve the appearance of the skin and/or hair comprising the simultaneous and/or sequential administration:a) of at least one cytochrome C oxidase substrate and/or of at least one agent which increases the expression of the said substrate; and b) of at least one light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase. In particular, the said light radiation exhibits at least one predominant wavelength which activates cytochrome C oxidase ranging from 550 to 1000 nm, in particular from 550 to 800 nm, preferably from 620 to 700 nm and more preferably still from 640 to 680 nm and is preferably used at a dose ranging from 0.01 to 200 J/cm2, preferably from 0.1 to 30 J/cm2, more preferably from 1 to 30 J/cm2, indeed even from 5 to 30 J/cm2.The invention also relates to a composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and at least one compound emitting and/or filtering, in particular under exposure to light, a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase, and to a kit comprising at least one composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and one device and/or one compound emitting/filtering a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase.

摘要翻译： 本发明更具体地涉及旨在特别改善皮肤和/或毛发外观的美容治疗方法，其包括同时和/或顺序给药：a）至少一种细胞色素C氧化酶底物和/或至少 一种增加所述基材表达的试剂; 和b）表现出至少一种主要波长的至少一种光辐射，其激活细胞色素C氧化酶。 特别地，所述光辐射表现出至少一个主要波长，其激活550至1000nm，特别是550至800nm，优选620至700nm，更优选仍为640至680nm的细胞色素C氧化酶，并且是 优选以0.01至200J / cm 2，优选0.1至30J / cm 2，更优选1至30J / cm 2，甚至5至30J / cm 2的剂量使用。 本发明还涉及一种组合物，其包含至少一种细胞色素C氧化酶底物和/或一种增加所述底物和至少一种特别是在光照下曝光和/或过滤的化合物的表达的试剂， 至少一个激活细胞色素C氧化酶的主要波长，以及包含至少一种包含至少一种细胞色素C氧化酶底物和/或一种增加所述底物表达的试剂的组合物的试剂盒，以及一种装置和/ /过滤表现出至少一种主要波长的光辐射，其激活细胞色素C氧化酶。

公开(公告)号：US20150087054A1

公开(公告)日：2015-03-26

申请号：US14323393

申请日：2014-07-03

申请人： Institut National De La Sante Et De La Recherche Medicale (Inserm)

发明人： Marisol CORRAL-DEBRINSKI ,  Jose-Alain SAHEL ,  Valerie KALTIMBACHER ,  Crystel BONNET

IPC分类号： C12N9/02 ,  C07K14/47 ,  C12N9/10

CPC分类号： C12N15/85 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0066 ,  C07K14/47 ,  C07K2319/07 ,  C12N9/0053 ,  C12N9/0089 ,  C12N9/1085 ,  C12N15/8509 ,  C12N2800/22 ,  C12N2810/80 ,  C12Y109/03001

摘要： An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.

公开(公告)号：US20240254436A1

公开(公告)日：2024-08-01

申请号：US18426809

申请日：2024-01-30

申请人： Alma Mater Studiorum - Università di Bologna

发明人： Stefania RAPINO ,  Francesco ZERBETTO ,  Gastone CASTELLANI ,  Stefano SALVIOLI ,  Beatrice FRABONI ,  Isabella ZIRONI ,  Maria CONTE ,  Claudio FRANCESCHI ,  Daniela SALVATORE ,  Maila BECCONI

IPC分类号： C12N5/00 ,  C12N5/09 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  G01N33/50

CPC分类号： C12N5/0018 ,  C12N5/0068 ,  C12N5/0693 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0036 ,  C12N9/0053 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0093 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  C12Y101/03004 ,  C12Y106/03001 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01006 ,  C12Y113/12004 ,  C12Y117/03002 ,  G01N33/5008 ,  C12N2500/02 ,  C12N2501/71 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2533/76 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2537/10

摘要： The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.

公开(公告)号：US20160208107A1

公开(公告)日：2016-07-21

申请号：US15083802

申请日：2016-03-29

申请人： Wanda M. Weder and William F. Straeter, not Individually but solely as Trustees of The Family

发明人： Donald E. Weder

IPC分类号： C09D5/00 ,  D21H23/22 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16 ,  C12N9/14

CPC分类号： C09D5/00 ,  B32B5/18 ,  B32B29/002 ,  C08J7/047 ,  C08J2300/16 ,  C12N9/0053 ,  C12N9/0055 ,  C12N9/0065 ,  C12N9/14 ,  C12N9/16 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01013 ,  C12Y111/01014 ,  D06N3/0056 ,  D06N7/00 ,  D06N2209/1614 ,  D21H23/22 ,  Y10T428/13 ,  Y10T428/1334 ,  Y10T428/24802 ,  Y10T428/249987 ,  Y10T428/31504 ,  Y10T428/31515 ,  Y10T428/31786 ,  Y10T428/3179 ,  Y10T428/31797 ,  Y10T428/31801 ,  Y10T428/31808 ,  Y10T428/31826 ,  Y10T428/31841 ,  Y10T428/31895 ,  Y10T428/31902 ,  Y10T428/31913 ,  Y10T428/31938 ,  Y10T428/31993 ,  Y10T428/31996 ,  Y10T442/2525

摘要： Methods of causing, enhancing, and/or expediting oxo-biodegradation of articles using agents of oxo-biodegradation are provided.

摘要翻译： 提供了使用氧代 - 生物降解剂引起，增强和/或加速制品的氧代降解的方法。

公开(公告)号：US09119798B2

公开(公告)日：2015-09-01

申请号：US12866575

申请日：2009-02-06

申请人： Julien Laboureau ,  Quang Lan Nguyen ,  Dang-Man Pham

发明人： Julien Laboureau ,  Quang Lan Nguyen ,  Dang-Man Pham

IPC分类号： A61K8/66 ,  A61N5/06 ,  A61K8/19 ,  A61K8/49 ,  A61Q7/00 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/08

CPC分类号： A61K41/00 ,  A61K8/19 ,  A61K8/494 ,  A61K8/66 ,  A61K38/1709 ,  A61K45/06 ,  A61K2800/81 ,  A61K2800/92 ,  A61N5/0617 ,  A61Q7/00 ,  A61Q19/007 ,  A61Q19/02 ,  A61Q19/08 ,  C12Y109/03001

摘要： The invention relates to the combination of a light ray with a cytochrome C oxidase substrate for improving the appearance of the skin and/or the hair.

摘要翻译： 本发明涉及光线与细胞色素C氧化酶底物的组合，用于改善皮肤和/或头发的外观。

公开(公告)号：US20150037865A1

公开(公告)日：2015-02-05

申请号：US14520022

申请日：2014-10-21

申请人： Wanda Weder & William Straeter, not individually but solely as Trustees of The Family Trust U/T/A d

发明人： Donald E. Weder

IPC分类号： C12N11/00 ,  B32B29/00 ,  B32B1/00 ,  B32B27/08 ,  B32B5/18 ,  B32B25/06 ,  B32B27/10 ,  B32B23/06 ,  B32B23/08 ,  B32B27/06 ,  B32B25/08

CPC分类号： C09D5/00 ,  B32B5/18 ,  B32B29/002 ,  C08J7/047 ,  C08J2300/16 ,  C12N9/0053 ,  C12N9/0055 ,  C12N9/0065 ,  C12N9/14 ,  C12N9/16 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01013 ,  C12Y111/01014 ,  D06N3/0056 ,  D06N7/00 ,  D06N2209/1614 ,  D21H23/22 ,  Y10T428/13 ,  Y10T428/1334 ,  Y10T428/24802 ,  Y10T428/249987 ,  Y10T428/31504 ,  Y10T428/31515 ,  Y10T428/31786 ,  Y10T428/3179 ,  Y10T428/31797 ,  Y10T428/31801 ,  Y10T428/31808 ,  Y10T428/31826 ,  Y10T428/31841 ,  Y10T428/31895 ,  Y10T428/31902 ,  Y10T428/31913 ,  Y10T428/31938 ,  Y10T428/31993 ,  Y10T428/31996 ,  Y10T442/2525

摘要： Kits and assemblies for causing, enhancing, and/or expediting consumption of an article by at least one biodegradative living organism are disclosed. The kits and assemblies include at least one additional living organism that enhances and/or expedites consumption of the article by the at least one biodegradative living organism. Methods of producing and using same are also provided.

摘要翻译： 公开了用于引起，增强和/或加速至少一种生物降解活生物消耗物品的组合件和组件。 试剂盒和装配体包括至少一种额外的生物体，其增强和/或加速由至少一种生物降解活生物体消耗制品。 还提供了生产和使用方法。