公开(公告)号：US09290735B2

公开(公告)日：2016-03-22

申请号：US12866588

申请日：2009-02-05

申请人： Masahiro Sokabe ,  Kishio Furuya ,  Go Tazaki ,  Hitoshi Tsuruta ,  Motohiro Fukuda

发明人： Masahiro Sokabe ,  Kishio Furuya ,  Go Tazaki ,  Hitoshi Tsuruta ,  Motohiro Fukuda

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12M1/32 ,  C12M3/06 ,  C12M1/00

CPC分类号： C12N5/0631 ,  C12M23/12 ,  C12M23/16 ,  C12M23/22 ,  C12N5/0062 ,  C12N2535/10

摘要： To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.

摘要翻译： 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞，并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时，可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器，其具有表面上的多个这种微量容器。

公开(公告)号：US20130071932A1

公开(公告)日：2013-03-21

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/095 ,  C12N5/076 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12M23/12 ,  C12N2502/28 ,  C12N2506/02 ,  C12N2535/10

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

摘要翻译： 提供了通过使用其中形成有多个微室的细胞培养室，实现胚状体大小的控制并且可以在控制胚状体大小的状态下诱导分化的方法。 用于引起多能哺乳动物细胞分化的培养方法使用在培养表面上形成有多个微室（11）的细胞培养室（10）。 细胞培养室（10）具有由空间形成的培养面，其中微室（11）具有高度为10μm〜500μm，底面积为100μm2〜1mm2的空间结构。 引起多能哺乳动物细胞分化的培养方法包括通过使用细胞培养室（10）培养多能哺乳动物细胞以获得至少部分分化为内胚层谱系细胞的细胞群。

公开(公告)号：US20100190253A1

公开(公告)日：2010-07-29

申请号：US12665474

申请日：2008-06-16

申请人： Go Tazaki ,  Tomoko Kosaka ,  Motohiro Fukuda

发明人： Go Tazaki ,  Tomoko Kosaka ,  Motohiro Fukuda

IPC分类号： C12N5/02 ,  C12M1/00

CPC分类号： C12M23/12 ,  B33Y80/00 ,  C12M23/16

摘要： To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber (10) according to the present invention is a cell culture chamber (10) including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.

摘要翻译： 提供能够有效地构建培养空间中的细胞间网络的细胞培养室和细胞培养方法。 根据本发明的细胞培养室（10）是包括形成在其表面上的多个微室（11）的细胞培养室（10），其特征在于，将微型室（11）分隔开的凸部（侧壁12） ）形成为防止细胞粘附到凸部的上表面。 因此，可以在微室（11）内专门培养细胞，并且可以有效地构建细胞间网络。

公开(公告)号：US20090149345A1

公开(公告)日：2009-06-11

申请号：US11908047

申请日：2006-03-01

申请人： Taiji Nishi ,  Motohiro Fukuda ,  Go Tazaki ,  Seiichi Kanai ,  Takenori Kitani ,  Naoto Fukuhara

发明人： Taiji Nishi ,  Motohiro Fukuda ,  Go Tazaki ,  Seiichi Kanai ,  Takenori Kitani ,  Naoto Fukuhara

IPC分类号： C40B30/10 ,  C40B40/00 ,  C40B50/00

CPC分类号： G01N33/80 ,  B01L3/502707 ,  B01L3/502746 ,  B01L2300/0681 ,  B01L2300/0816 ,  G01N33/56972

摘要： A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel.

摘要翻译： 微通道阵列，其制造方法和血液检查方法。 微通道阵列通过接合第一和第二基底而形成，每个基底在其表面上包括流体入口和出口。 内部空间结构连接流体入口和出口，并且包括与流体入口连接的上游流动通道，与流体出口连接的下游流动通道，其间具有间隙，以及连接上游和下游流动通道的微流动通道。 从微流动通道的截面的中心到微流动通道的侧壁的最小距离小于上游和下游流动通道的最小距离。 第一和第二基板的每个表面包括用于产生上游和下游流动通道的槽，并且第二基板的表面具有用于产生微流动通道的凹槽。

公开(公告)号：US20070225418A1

公开(公告)日：2007-09-27

申请号：US11587921

申请日：2005-04-25

申请人： Hiroshi Ozawa ,  Yuichi Katoh ,  Motohiro Fukuda

发明人： Hiroshi Ozawa ,  Yuichi Katoh ,  Motohiro Fukuda

IPC分类号： C08L23/08 ,  C08L33/10 ,  C08L51/00

CPC分类号： C08L51/06 ,  C08F255/02 ,  C08F255/026 ,  C08L23/0853 ,  C08L2666/24 ,  C08L2666/04 ,  C08L2666/02

摘要： To provide a thermoplastic elastomer composition which is excellent in light resistance and flexibility and has high tear strength. [MEANS FOR SOLVING PROBLEMS] A thermoplastic elastomer composition comprising 100 parts by mass of acrylic multi-layer polymer particles (A) which each comprise at least two layers, i.e., at least one inner rubber layer (I) and at least one outermost thermoplastic resin layer (II), 5 to 300 parts by mass of an ethylene/vinyl ester copolymer (B), and 0.5 to 150 parts by mass of a graft copolymer (C) composed of an ethylene/vinyl ester copolymer backbone and a graft bonded to the backbone which consists of units derived from a monomer having a polarity higher than that of the backbone.

摘要翻译： 提供耐光性和柔软性优异且撕裂强度高的热塑性弹性体组合物。 [解决问题的手段]一种热塑性弹性体组合物，其包含100质量份丙烯酸多层聚合物颗粒（A），其各自包含至少两层，即至少一层内橡胶层（I）和至少一种最外层热塑性塑料 树脂层（II），乙烯/乙烯基酯共聚物（B）5〜300质量份，乙烯/乙烯基酯共聚物主链和接枝共聚物主链组成的接枝共聚物（C）0.5〜150质量份 到由具有高于骨架的极性的单体衍生的单元组成的骨架。

公开(公告)号：US20070202589A1

公开(公告)日：2007-08-30

申请号：US11360535

申请日：2006-02-24

申请人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

发明人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

IPC分类号： C12M3/00

CPC分类号： C12M23/16 ,  B01L3/5027

摘要： A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.

摘要翻译： 细胞培养板具有多个流路，不均匀图案区域和通孔。 流动通道形成在不均匀图案区域之间，培养液从流动通道流入不均匀图案区域或从凹凸图案区域流入流动通道。 不均匀图案区域具有形成细胞培养空间的不均匀图案。

公开(公告)号：US08652833B2

公开(公告)日：2014-02-18

申请号：US12298803

申请日：2007-04-25

申请人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

发明人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

IPC分类号： C12M3/00 ,  B05D5/10 ,  C40B40/00 ,  C04B40/02

CPC分类号： C12M23/12 ,  C12M23/16

摘要： To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.

摘要翻译： 提供能够在体内再现细胞功能的细胞培养室及其制造方法。 根据本发明的细胞培养室具有形成在其上培养细胞的表面上的凹凸图案。 凹凸图案的凹部包括与细胞培养部连通的细胞培养部和微流路。 每个细胞培养部分的底表面具有每个培养细胞的当量直径的1.0至20倍的宽度。 细胞粘附诱导物质仅形成在凹部的底面和侧壁上。

公开(公告)号：US20120199816A1

公开(公告)日：2012-08-09

申请号：US13390271

申请日：2010-08-02

申请人： Go Tazaki ,  Motohiro Fukuda ,  Hiroyuki Ohgi ,  Akio Fujita

发明人： Go Tazaki ,  Motohiro Fukuda ,  Hiroyuki Ohgi ,  Akio Fujita

IPC分类号： H01L51/54 ,  H01L51/48 ,  H01L51/46 ,  H01L51/56

CPC分类号： H01L51/4253 ,  H01L51/0026 ,  H01L51/0036 ,  H01L51/0037 ,  H01L51/424 ,  H01L51/447 ,  H01L2251/105 ,  H01L2251/308 ,  Y02E10/549 ,  Y02P70/521

摘要： A photoelectric conversion device according to the present invention includes, between a pair of electrodes, an electron donor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting electrode main surfaces being formed at intervals in the stripe-like part in cross section; and an electron acceptor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting the electrode main surfaces being formed at intervals in the stripe-like part in cross section, the photoelectric conversion device further including an active layer in which the plurality of strip-like parts in cross section of the electron donor layer and the plurality of strip-like parts in cross section of the electron acceptor layer are alternately joined. A stripe width a of the stripe-like part in cross section of the electron donor layer and a stripe width b of the stripe-like part in cross section of the electron acceptor layer are both 5 to 100 nm. When a=b, a thickness c of the active layer is twice to 40 times as large as a (=b). When a≠b, the thickness c of the active layer is twice or more of one of a and b which is smaller and 40 times or less of one of a and b which is larger.

摘要翻译： 根据本发明的光电转换装置包括在一对电极之间，具有横截面的交错形状的电子供体层，包括横截面的条状部分和基部，多个条状部分交叉 横截面中在与条状部分间隔地形成与电极主表面交叉的方向延伸的部分; 以及具有交叉形状的电子受体层，其截面包括条状部分和基部，沿着与电极主表面相交的方向延伸的多个条状部分形成在 光电转换装置还包括有源层，其中电子供体层的横截面中的多个条状部分和电子受体层的横截面中的多个条状部分 交替连接。 电子供体层的横截面中的条状部分的条纹宽度a和电子受体层横截面中的条状部分的条带宽度b均为5〜100nm。 当a = b时，有源层的厚度c为（= b）的两倍至40倍。 当a≠b时，有效层的厚度c是a和b中的一个的两倍或更多，其中a和b之一更小，并且a和b之一更大的是40倍或更少。

公开(公告)号：US20110217725A1

公开(公告)日：2011-09-08

申请号：US13125896

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/07 ,  C12Q1/02

CPC分类号： C12N5/0062 ,  C12M23/12 ,  C12N2502/00 ,  C12N2503/02 ,  C12N2513/00

摘要： To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

摘要翻译： 提供包括各种供体的培养的活细胞的细胞培养试剂盒及其制造方法。 细胞培养试剂盒包括培养板和在其上培养的活细胞。 培养板包括多个微室（33），并且来自各种供体的活细胞粘附到多个微室（33）的表面。 具体而言，将来自各种供体的活细胞D1，D2和D3粘附到多个微室（33）。 在每个微室（33）中，可以培养衍生自一个供体的活细胞或来自各种供体的活细胞。 来自各种供体的活细胞作为整体在细胞培养试剂盒中附着和培养，这使得可以提供使用来自各种供体的细胞进行测试的细胞培养试剂盒。

公开(公告)号：US07972543B2

公开(公告)日：2011-07-05

申请号：US11577474

申请日：2005-10-11

申请人： Mitsutoshi Nakajima ,  Taiji Nishi ,  Seiichi Kanai ,  Takenori Kitani ,  Motohiro Fukuda

发明人： Mitsutoshi Nakajima ,  Taiji Nishi ,  Seiichi Kanai ,  Takenori Kitani ,  Motohiro Fukuda

IPC分类号： B29B9/00

CPC分类号： B01J13/04 ,  A23P10/25 ,  A61K9/1682 ,  B01F3/0807 ,  B01F5/0475 ,  B01F13/0059 ,  B01F2215/0014 ,  B01F2215/0016 ,  B01F2215/0019 ,  B01F2215/0031 ,  B01F2215/0034 ,  B01J13/02 ,  C25D1/08 ,  Y10T428/24273

摘要： An object of the present invention is to provide a process for producing microspheres that are solid microparticles or liquid microparticles for use as an emulsion employed in the food industry, production of medicine and cosmetic etc. or an emulsion for DDS (drug delivery system). The object is attained by a process for producing a microsphere including: separating a disperse phase from a continuous phase by a substrate 1 having a through-hole 7; and extruding the disperse phase into the continuous phase through the through-hole 7, in which the substrate having the through-hole 7 with a width of 0.5 to 500 μm, a depth of 10 μm to 6000 μm and a ratio of the width to the depth of the through-hole 7 of 1 to 1/30 is a metal substrate.

摘要翻译： 本发明的目的是提供一种制备用作食品工业中使用的乳液的固体微粒或液体微粒的微球的制造方法，药物和化妆品的制造等，或用于DDS（药物输送系统）的乳液。 该目的通过一种制备微球体的方法来实现，包括：通过具有通孔7的基底1将分散相与连续相分离; 并且通过通孔7将分散相挤出到连续相中，其中具有宽度为0.5至500μm的通孔7的基底，深度为10μm至6000μm，宽度与 通孔7的深度为1〜1/30是金属基板。