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    Method for detecting and quantifying rare mutations/polymorphisms
    21.
    发明授权
    Method for detecting and quantifying rare mutations/polymorphisms 有权
    检测和定量稀有突变/多态性的方法

    公开(公告)号：US07709262B2

    公开(公告)日：2010-05-04

    申请号：US10589709

    申请日：2005-02-18

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

    Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

    Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

    Nucleic acid supported protein complementation
    22.
    发明授权
    Nucleic acid supported protein complementation 有权
    核酸支持的蛋白质互补

    公开(公告)号：US07662554B2

    公开(公告)日：2010-02-16

    申请号：US10529122

    申请日：2003-10-09

    Applicant: Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffmann

    Inventor： Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffmann

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

    Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

    Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

    Streptavidin proteins
    24.
    发明授权
    Streptavidin proteins 失效
    链霉亲和素蛋白

    公开(公告)号：US07179618B2

    公开(公告)日：2007-02-20

    申请号：US10285876

    申请日：2002-11-01

    Applicant: Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    Inventor： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC: C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C12P21/06

    CPC classification number: C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    Screening assay for the detection of DNA-binding molecules
    26.
    发明授权
    Screening assay for the detection of DNA-binding molecules 失效
    检测DNA结合分子的筛选试验

    公开(公告)号：US5726014A

    公开(公告)日：1998-03-10

    申请号：US123936

    申请日：1993-09-17

    Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin

    Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin

    IPC: C12N15/09 ,  C07K14/035 ,  C07K14/47 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34 ,  G01N33/566

    CPC classification number: C12Q1/6811 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6883 ,  C12Q1/705

    Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

    Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

    DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof
    27.
    发明授权
    DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof 失效
    编码链霉亲和素的DNA，由此产生的链霉抗生物素蛋白，包括存在于链霉亲和素中的氨基酸序列的融合多肽及其用途

    公开(公告)号：US4839293A

    公开(公告)日：1989-06-13

    申请号：US833324

    申请日：1986-02-24

    Applicant: Charles R. Cantor ,  Richard Axel ,  Carlos Argarana

    Inventor： Charles R. Cantor ,  Richard Axel ,  Carlos Argarana

    IPC: C12N15/09 ,  C07K14/36 ,  C07K14/705 ,  C12N1/20 ,  C12N5/10 ,  C12P21/02 ,  C12R1/19 ,  C12R1/465 ,  C12R1/91

    CPC classification number: C07K14/36 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/22 ,  C07K2319/33 ,  Y10S930/30

    Abstract: DNA which encodes the polypeptide streptavidin has been isolated as a fragment 2 kb in length derived from a restriction endonuclease digestion of the chromosomal DNA of Streptomyces avidinii. The nucleic acid sequence of the gene and the amino acid sequence of the polypeptide have been determined. A fused gene has been prepared which comprises the streptavidin gene fused to a gene encoding the human LDL receptor. Expression of the gene fusion results in a fused streptavidin-human LDL receptor polypeptide. Methods are provided for using the fused gene to produce labeled, chemically modified proteins in vivo and to isolate a protein knowing only the nucleotide sequence of the gene encoding the protein.

    Abstract translation: 编码多肽链霉抗生物素蛋白的DNA已经被分离为长度为2kb的片段，其来源于阿维链霉菌的染色体DNA的限制性内切核酸酶消化。 已经确定了该基因的核酸序列和该多肽的氨基酸序列。 已经制备了融合基因，其包含与编码人LDL受体的基因融合的链霉亲和素基因。 基因融合的表达导致融合的链霉亲和素 - 人LDL受体多肽。 提供了使用融合基因在体内产生标记的，化学修饰的蛋白质并分离仅知道编码蛋白质的基因的核苷酸序列的蛋白质的方法。

    Quantification of Gene Expression
    28.
    发明申请
    Quantification of Gene Expression 有权
    基因表达的定量

    公开(公告)号：US20120028838A1

    公开(公告)日：2012-02-02

    申请号：US13223923

    申请日：2011-09-01

    Applicant: Charles R. Cantor ,  Chunming Din

    Inventor： Charles R. Cantor ,  Chunming Din

    IPC: C12Q1/68 ,  C40B30/10

    CPC classification number: C12Q1/6851 ,  C12Q2545/107 ,  C12Q2535/125 ,  C12Q2525/186

    Abstract: The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.

    Abstract translation: 本发明涉及使用与目的基因相比具有一个碱基差异的标准或“靶核酸序列”来测量样品中靶核酸量的方法。使用这种标准物 结合使用例如在突变位点上携带的碱基延伸反应来“提高”标准差和测试核酸样品的方法，允许以相同的效率扩增标准品和靶核酸并促进定量 的靶核酸。 此后，使用定量“增强”标准和靶核酸样品的手段来确定靶核酸的量。 在优选实施方案中，定量方法是质谱法。

    SUPRAMOLECULAR BIOCONJUGATES
    29.
    发明申请
    SUPRAMOLECULAR BIOCONJUGATES 审中-公开

    公开(公告)号：US20100255558A1

    公开(公告)日：2010-10-07

    申请号：US12435665

    申请日：2009-05-05

    Applicant: Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    Inventor： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    IPC: C07K17/00 ,  C07K1/00 ,  C12N9/96

    CPC classification number: B82Y5/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/557 ,  A61K47/6949 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682

    Abstract: The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurality of bioreactive complexes. The plurality of bioreactive complexes are hybridized to the plurality of first nucleic acids to form the supramolecular bioconjugate. Bioconjugates can be used to detect and isolate targets, to screen samples for targets such as antigens, to treat patients with multiple agents or to diagnose disorders in the form of a kit.

    Abstract translation: 本发明涉及超分子生物缀合物和组分和利用超分子生物缀合物的方法。 超分子生物缀合物包含多个第一核酸和多个介体，其中每个介体包含与所述多个第一核酸内的序列互补的第二核酸。 为了组装超分子生物缀合物，将一组或多组生物反应剂偶联至多个介体，形成多个生物反应性复合物。 多个生物反应性复合物与多个第一核酸杂交以形成超分子生物缀合物。 生物共轭物可用于检测和分离靶标，筛选靶标如抗原的样品，以治疗患有多种药物的患者或以试剂盒的形式诊断疾病。

    Methods for prenatal diagnosis of chromosomal abnormalities
    30.
    发明申请
    Methods for prenatal diagnosis of chromosomal abnormalities 有权
    产前诊断染色体异常的方法

    公开(公告)号：US20090325232A1

    公开(公告)日：2009-12-31

    申请号：US12553225

    申请日：2009-09-03

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: C12P19/30

    CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q2521/331

    Abstract: Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

    Abstract translation: 染色体异常负责大量的出生缺陷，包括精神发育迟滞。 本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。 本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。 本文描述的方法可用于检测染色体DNA缺失和重复。 在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

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