公开(公告)号：US10281468B2

公开(公告)日：2019-05-07

申请号：US15118850

申请日：2015-02-25

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  G01N30/88 ,  C12Q1/04 ,  C12Q1/02 ,  C12Q1/68 ,  G01N30/72 ,  C12Q1/686 ,  G01N33/68

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to select a peptide labeled with a first tag of a known microbe, fragment the labeled peptide of the known microbe, and monitor for an intensity of the first tag in an MRM method using a processor. An ion source provides a beam of ions from a sample that includes peptides labeled with the first tag. The first tag binds to a peptide of a known microbe and is cleaved from the peptide of the known microbe during mass spectrometry. The mass spectrometer receives the beam of ions and is adapted to perform the MRM method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample using the processor.

公开(公告)号：US20140349885A1

公开(公告)日：2014-11-27

申请号：US14368358

申请日：2012-12-06

Applicant: DH Technologies development Pte. Ltd.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/74 ,  G01N33/58

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2030/042 ,  G01N2458/15 ,  G01N2560/00 ,  Y10T436/200833

Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供酮或醛化合物的相对定量，绝对定量或两者的方法，标记试剂，组标记试剂和标记技术，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物基质中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US20140127825A1

公开(公告)日：2014-05-08

申请号：US14104117

申请日：2013-12-12

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。

公开(公告)号：US20250136810A1

公开(公告)日：2025-05-01

申请号：US18683343

申请日：2022-08-11

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: C08L71/02 ,  H01J49/00

Abstract: The disclosure provides compositions, methods, and kits that find use in calibrating a mass spectrometer, and can include one or more predetermined concentration(s) of one or more calibrant molecule(s) that comprise a polyethylene glycol (PEG) compounds that have a single functional group that can be ionized by an ion source, and a solvent for dissolving the calibrant molecule(s). The calibrant molecule(s) and compositions including them can be used in either positive or negative ionization mode, and can be used for calibrating a variety of mass spectrometers (e.g., APCI, ESI) operating in a variety of acquisition modes (e.g., MRM, MS/MS, etc.).

公开(公告)号：US20240420939A1

公开(公告)日：2024-12-19

申请号：US18704242

申请日：2022-10-26

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Michal Weinstock ,  Yongquan Lai ,  Subhasish Purkayastha ,  Scott Daniels

IPC: H01J49/04 ,  H01J49/00

Abstract: The presently claimed and described technology provides a sample processing system comprising at least one sample introduction device, wherein the at least one sample introduction device is configured to receive a sample; a mass analyzer coupled to the sample introduction device; a control system configured to at least control the at least one sample introduction device and/or the mass analyzer, wherein the mass analyzer is configured to perform a first mass analysis on the sample, wherein the first mass analysis is mass screening for an analyte of interest in the sample, and wherein if the analyte of interest is detected in the sample, the mass analyzer is configured to perform a second mass analysis, wherein the second mass analysis is a quantitative analysis, comprising: ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte; determining intensity and/or abundance of the at least one product ion transition and/or the at least one isotopic ion transition; and quantifying the at least one analyte present in the sample using the intensity and/or abundance of the at least one product ion transition and/or isotopic ion transition.

公开(公告)号：US20240177984A1

公开(公告)日：2024-05-30

申请号：US18283378

申请日：2022-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Bradley Schneider ,  Leigh Bedford ,  Aaron Stella ,  Subhasish Purkayastha

IPC: H01J49/04 ,  G01N1/38 ,  G01N27/624 ,  G01N33/487

CPC classification number: H01J49/0454 ,  G01N1/38 ,  G01N27/624 ,  G01N33/487

Abstract: Methods of detecting at least one analyte in at least one liquid sample are described. The method includes the steps of desalting the liquid sample, acoustically ejecting the desalted sample into an open-port interface, diluting the desalted sample, and transferring the diluted sample to an ionization source, ionizing the diluted sample, and selecting ions of interest by ion mobility

公开(公告)号：US20240168040A1

公开(公告)日：2024-05-23

申请号：US18283375

申请日：2022-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Chang Liu ,  Yuguan Liu ,  Subhasish Purkayastha

IPC: G01N33/94 ,  C12Q1/37 ,  G01N30/02 ,  G01N30/72 ,  G01N33/543

CPC classification number: G01N33/94 ,  C12Q1/37 ,  G01N30/724 ,  G01N33/54326 ,  G01N33/54353 ,  G01N2030/027 ,  G01N2333/96477 ,  G01N2333/976

Abstract: Methods and kits for preparing liquid samples are presently claimed and described. The method may include treating liquid sample with a hydrolysis enzyme, hydrolyzing the liquid sample to prepare a hydrolysate, and purifying the hydrolysate with magnetic based purification. In certain aspects, the hydrolysis enzyme is bound to a magnetic bead or a magnetic particle. Kits for preparing a liquid sample can include a hydrolysis enzyme, magnetic beads or magnetic particles, one or more internal standards, a liquid chromatography column and one or more solvents to be used as mobile phases, one or more calibrant solutions and instructions for use.

公开(公告)号：US11988670B2

公开(公告)日：2024-05-21

申请号：US17814530

申请日：2022-07-25

Applicant: BECKMAN COULTER, INC. ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Hudson ,  Takayuki Mizutani ,  Subhasish Purkayastha ,  Thomas W. Roscoe

IPC: G01N33/68 ,  G01N27/624 ,  H01J49/00 ,  H01J49/02

CPC classification number: G01N33/6848 ,  G01N27/624 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/025

Abstract: An integrated sample processing system including an analyzer and a mass spectrometer is disclosed. The integrated sample processing system can perform multiple different types of detection, thereby providing improved flexibility and better accuracy in processing samples. The detection systems in the sample processing system may include an optical detection system and a mass spectrometer.

公开(公告)号：US20230236189A1

公开(公告)日：2023-07-27

申请号：US18151974

申请日：2023-01-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/88 ,  G01N33/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

公开(公告)号：US10656147B2

公开(公告)日：2020-05-19

申请号：US15121803

申请日：2015-02-27

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L. Campbell ,  Thomas R. Covey ,  Chang Liu ,  Subhasish Purkayastha

IPC: G01N33/553 ,  G01N33/543 ,  G01N1/38 ,  B01F13/00 ,  B01F13/08 ,  G01N35/00 ,  B01L3/00 ,  G01N27/74

Abstract: Methods and apparatus for processing fluids on a macro- or micro-scale are described. In various aspects, a fluid may have a plurality of elongated (i.e., substantially rod-shaped) magnetic elements disposed therein within a fluid container. An illustrative fluid container is an actuator electrode or a processing vial of a microfluidic device, such as a digital microfluidic device. A magnet component may be configured to generate a magnetic force sufficient to influence the movement of the plurality of elongated magnetic elements within the fluid to be processed. For example, the magnetic force (or magnetic force gradient) may influence the plurality of elongated magnetic elements to rotate, spin, and/or move laterally side-to-side. The shape and movements of the plurality of elongated magnetic elements facilitate the rapid and efficient processing of the fluid, such as fluid mixing and/or fluid separation.