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21.发明申请公开(公告)号：US20130295602A1
公开(公告)日：2013-11-07
申请号：US13781307
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: C12Q1/68
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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公开(公告)号：US20220017953A1
公开(公告)日：2022-01-20
申请号：US17333792
申请日：2021-05-28
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
IPC: C12Q1/6851 , C12Q1/6806 , C12Q1/70
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
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公开(公告)号：US20210268508A1
公开(公告)日：2021-09-02
申请号：US17181966
申请日：2021-02-22
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
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公开(公告)号：US20200264205A1
公开(公告)日：2020-08-20
申请号：US16752073
申请日：2020-01-24
Applicant: Fluidigm Corporation
Inventor： Jason A. A. West , Brian Fowler
Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.
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25.发明申请公开(公告)号：US20180306683A1
公开(公告)日：2018-10-25
申请号：US15925600
申请日：2018-03-19
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: G01N1/28 , C12P19/34 , C12Q1/686 , C12Q1/6813 , C12Q1/6844 , C12Q1/6869 , G01N1/34 , G01N15/14
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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Patent Agency Ranking
公开(公告)号：US09952126B2
公开(公告)日：2018-04-24
申请号：US13781313
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C Norris , Dominique Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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公开(公告)号：US09498776B2
公开(公告)日：2016-11-22
申请号：US14720305
申请日：2015-05-22
Applicant: Fluidigm Corporation
Inventor： David Cohen , Andrew May , Martin Pieprzyk , Brian Fowler , Kim Huat Lee , Jun Yan , Ming Fang Zhou , Seng Beng Ng
CPC classification number: B01L3/5027 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/12 , B01L2300/045 , B01L2300/0887 , B01L2300/123 , B01L2400/0655 , G01N1/405
Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.
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公开(公告)号：US20160208322A1
公开(公告)日：2016-07-21
申请号：US14723872
申请日：2015-05-28
Applicant: Fluidigm Corporation
Inventor： Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
CPC classification number: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
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公开(公告)号：US09383295B2
公开(公告)日：2016-07-05
申请号：US14477642
申请日：2014-09-04
Applicant: Fluidigm Corporation
Inventor： Martin Pieprzyk , Geoff Facer , Timothy Woudenberg , Brian Fowler
CPC classification number: G01N1/28 , B01L3/502738 , B01L7/52 , B01L2200/16 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , C12Q1/686 , G01N21/05 , G01N21/6452 , G01N2021/0346 , Y10T137/7793 , Y10T436/2575
Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.
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公开(公告)号：US20140318633A1
公开(公告)日：2014-10-30
申请号：US14091342
申请日：2013-11-27
Applicant: Fluidigm Corporation
Inventor： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
IPC: F16K99/00
CPC classification number: F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
Abstract translation: 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。
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