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    ALCOHOL DEHYDROGENASE VARIANTS
    22.
    发明公开
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开

    公开(公告)号：US20230159902A1

    公开(公告)日：2023-05-25

    申请号：US17861036

    申请日：2022-07-08

    Applicant: Genomatica, Inc.

    Inventor： Stefan ANDRAE ,  Michael Patrick KUCHINSKAS ,  Jingyi LI ,  Harish NAGARAJAN ,  Priti PHARKYA

    IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

    CPC classification number: C12N9/0006 ,  C12P7/24 ,  C12Y101/01244 ,  C12P7/18 ,  Y02P20/10 ,  C12Y101/01001

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Microorganisms for Producing 1,3-Butanediol and Methods Related Thereto
    27.
    发明申请
    Microorganisms for Producing 1,3-Butanediol and Methods Related Thereto 审中-公开
    用于生产1,3-丁二醇的微生物及其相关方法

    公开(公告)号：US20160076060A1

    公开(公告)日：2016-03-17

    申请号：US14692630

    申请日：2015-04-21

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN ,  Priti PHARKYA

    IPC: C12P7/18

    CPC classification number: C12P7/18 ,  C12N15/52

    Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    Abstract translation: 本文提供了具有1,3-丁二醇（1,3-BDO）途径的非天然存在的微生物生物，并且包含至少一种编码1,3-BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3-BDO。 在一些实施方案中，该途径包括从CO或氢中还原当量。 在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。 本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF CAPROLACTONE
    28.
    发明申请
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF CAPROLACTONE 审中-公开
    用于生产CAPROLACTONE的微生物和方法

    公开(公告)号：US20150353975A1

    公开(公告)日：2015-12-10

    申请号：US14596072

    申请日：2015-01-13

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Mark J. BURK

    IPC: C12P17/08 ,  C07D313/04 ,  C12N15/70

    CPC classification number: C12P17/08 ,  C07D313/04 ,  C08G18/4277 ,  C08G63/08 ,  C12N5/0018 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P17/02

    Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

    Abstract translation: 本发明提供了非天然存在的含有己内酯途径的微生物，其具有至少一种编码丁二烯途径酶的外源核酸，其表达量足以产生己内酯。 本发明另外提供了使用这种微生物生物体来制备己内酯的方法，该方法是在条件和足够的时间内培养含本文所述的己内酯途径的非天然存在的微生物生物产生己内酯。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE
    30.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE 审中-公开

    公开(公告)号：US20180363012A1

    公开(公告)日：2018-12-20

    申请号：US15600479

    申请日：2017-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. BURK ,  Anthony P. BURGARD ,  Priti PHARKYA

    IPC: C12P7/46 ,  C12N9/88 ,  C12P7/40

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

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